Design and Evaluation of an Automated System for In Vitro Dissolution Testing Utilizing a High-Pressure Liquid Chromatographic Multiport Switching Valve

1977 ◽  
Vol 66 (12) ◽  
pp. 1665-1670 ◽  
Author(s):  
John S. Kent ◽  
Paul P. Wong ◽  
G.P. Hegde
Keyword(s):  
High Pressure ◽  
Automated System ◽  
In Vitro Dissolution ◽  
Dissolution Testing ◽  
High Pressure Liquid Chromatographic ◽  
Switching Valve ◽  
Liquid Chromatographic

An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c.

1984 ◽  
Vol 30 (11) ◽  
pp. 1746-1752 ◽  
Author(s):  
G Ellis ◽  
E P Diamandis ◽  
E E Giesbrecht ◽  
D Daneman ◽  
L C Allen
Keyword(s):  
High Pressure ◽  
Ion Exchange ◽  
Hemoglobin A1c ◽  
Cation Exchanger ◽  
Automated System ◽  
Polyaspartic Acid ◽  
High Pressure Liquid Chromatographic ◽  
Switching Valve ◽  
Nacl Concentration ◽  
Liquid Chromatographic

Abstract An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c is described. We use a 45-min incubation in acetate buffer (pH 5.5) to eliminate labile glycated hemoglobins. In this automated system conventional modules are used but it incorporates a solvent-switching valve to select either of two buffers, which differ in pH and NaCl concentration. The chromatographic column contains "polyCAT" (a weak cation-exchanger, polyaspartic acid linked to silica). Run time is 14 min per sample. The method is precise and results correlate well with those by other ion-exchange procedures.

Switching Valve Designed For High Pressure Liquid Chromatographic Fraction Collection

1979 ◽  
Vol 62 (6) ◽  
pp. 1355-1357
Author(s):  
William O Landen
Keyword(s):  
High Pressure ◽  
Oil Removal ◽  
High Pressure Liquid Chromatographic ◽  
Liquid Chromatograph ◽  
Switching Valve ◽  
Gel Permeation ◽  
In Series ◽  
High Pressure Liquid Chromatograph ◽  
Pass Through ◽  
Liquid Chromatographic

Abstract The use of a collection valve specifically designed for high pressure liquid chromatography is described. Application of the valve to high pressure gel permeation chromatographic (GPC) separation of oil from the vitamin A active fraction of margarine resulted in efficient oil removal after one pass through 2 μStyragel (100A) columns connected in series. Using the normal collection mode of the high pressure liquid chromatograph, 2 passes through the GPC columns were required to adequately resolve the fractions.

scholarly journals In vitro dissolution testing models of ocular implants for posterior segment drug delivery

2021 ◽  
Author(s):  
Muhammad Faris Adrianto ◽  
Febri Annuryanti ◽  
Clive G. Wilson ◽  
Ravi Sheshala ◽  
Raghu Raj Singh Thakur
Keyword(s):  
Drug Release ◽  
Posterior Segment ◽  
In Vitro Dissolution ◽  
Prolonged Treatment ◽  
Term Therapy ◽  
In Vitro Test ◽  
Dissolution Testing ◽  
Vitro Test ◽  
Ocular Implants

AbstractThe delivery of drugs to the posterior segment of the eye remains a tremendously difficult task. Prolonged treatment in conventional intravitreal therapy requires injections that are administered frequently due to the rapid clearance of the drug molecules. As an alternative, intraocular implants can offer drug release for long-term therapy. However, one of the several challenges in developing intraocular implants is selecting an appropriate in vitro dissolution testing model. In order to determine the efficacy of ocular implants in drug release, multiple in vitro test models were emerging. While these in vitro models may be used to analyse drug release profiles, the findings may not predict in vivo retinal drug exposure as this is influenced by metabolic and physiological factors. This review considers various types of in vitro test methods used to test drug release of ocular implants. Importantly, it discusses the challenges and factors that must be considered in the development and testing of the implants in an in vitro setup. Graphical abstract

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