Creatinine IX: Specificity and Sensitivity of High-Performance Liquid Chromatographic and Ion-Exchange Membrane Methods for Determination of Endogenous Creatinine

1979 ◽  
Vol 68 (6) ◽  
pp. 802-803 ◽  
Author(s):  
Win L. Chiou
Keyword(s):  
Ion Exchange ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Ion Exchange Membrane ◽  
Specificity And Sensitivity ◽  
Exchange Membrane ◽  
Liquid Chromatographic ◽  
Membrane Methods

scholarly journals A VALIDATED ANALYTICAL HPLC METHOD FOR THE QUANTIFICATION OF LINCOMYCIN HYDROCHLORIDE IN BULK AND SOLID DOSAGE FORM

2017 ◽  
Vol 9 (3) ◽  
pp. 42
Author(s):  
P. Rajeev Kumar ◽  
Rekha Rajeevkumar
Keyword(s):  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Hplc Method ◽  
Solid Dosage ◽  
Rp Hplc ◽  
Specificity And Sensitivity ◽  
Ich Guidelines ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Objective: Develop a simple isocratic reverse phase high performance liquid chromatographic method (RP-HPLC) and validate for the determination of lincomycin hydrochloride (LMH) in bulk and pharmaceutical preparations.Methods: RP-HPLC quantification was carried out by using fine pack SIL RPC18 column. The mobile phase (methanol: water) was pumped at a flow rate of 1 ml/min in the ratio of 90:10 v/v and the eluents were monitored at 254 nm.Results: The retention time of the drug was 3.73 min and produced at a linear response in the concentration range of 5-25µg/ml. The percentage RSD was found to be below 2%. The LOD and LOQ were found to be 0.854µg/ml and 0.258µg/ml respectively.Conclusion: Validation of the method was performed for precision, accuracy, linearity, ruggedness, specificity and sensitivity to conform to ICH guidelines for valuation for analytical methods.

Sign in / Sign up

Export Citation Format

Share Document