The objective of this experiment was to test the effects of combining plant extracts rich in tannins and saponins at varying proportions on in vitro ruminal methane and ammonia formation. Tannins were extracted from Swietenia mahogani leaves and saponins from Sapindus rarak fruits with various solvents. The extracts obtained with the most efficient solvents (tannins: 75% water and 25% methanol; saponins: pure methanol) were then used in vitro. The treatments consisted of two substrate types (high-forage (HF) or high-concentrate (HC) diets) and five extract combinations (tannins: saponins, 1:0, 3:1, 1:1, 1:3, and 0:1) added at 2 mg/mL in incubation liquid. In vitro incubation was performed in four runs, with each treatment being represented with two replicates per run. The addition of plant extracts rich in tannins and saponins, either individually or in combination, decreased the methane proportion of total gas in both the HF (p < 0.05) and HC (p < 0.05) diets. The effects of the plant extracts rich in tannins and saponins were generally additive in mitigating methane emissions. Favorable associative effects between the extracts were observed in the ammonia concentration, both in the HF (p < 0.001) and HC (p < 0.01) diets and in the methane proportion of total gas, with a 1:3 mixture of tannins and saponins added to the HC diet (p < 0.05).
In vitro
screening of natural feed additives from crustaceans, diatoms, seaweeds and plant extracts to manipulate rumen fermentation