In vitro screening of American plant extracts on Trypanosoma cruzi and Trichomonas vaginalis

2000 ◽  
Vol 71 (1-2) ◽  
pp. 101-107 ◽  
Author(s):  
S Muelas-Serrano ◽  
J.J Nogal ◽  
R.A Martı́nez-Dı́az ◽  
J.A Escario ◽  
A.R Martı́nez-Fernández ◽  
...  
Phytomedicine ◽  
2005 ◽  
Vol 12 (6-7) ◽  
pp. 424-432 ◽  
Author(s):  
C.P. Serra ◽  
S.F. Côrtes ◽  
J.A. Lombardi ◽  
A. Braga de Oliveira ◽  
F.C. Braga

2013 ◽  
Vol 94 (6) ◽  
pp. 1191-1196 ◽  
Author(s):  
Zoey Durmic ◽  
Peter J Moate ◽  
Richard Eckard ◽  
Dean K Revell ◽  
Richard Williams ◽  
...  

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1020
Author(s):  
Rajaraman Bharanidharan ◽  
Selvaraj Arokiyaraj ◽  
Myunggi Baik ◽  
Ridha Ibidhi ◽  
Shin Ja Lee ◽  
...  

Indiscriminate use of antibiotics can result in antibiotic residues in animal products; thus, plant compounds may be better alternative sources for mitigating methane (CH4) production. An in vitro screening experiment was conducted to evaluate the potential application of 152 dry methanolic or ethanolic extracts from 137 plant species distributed in East Asian countries as anti-methanogenic additives in ruminant feed. The experimental material consisted of 200 mg total mixed ration, 20 mg plant extract, and 30 mL diluted ruminal fluid-buffer mixture in 60 mL serum bottles that were sealed with rubber stoppers and incubated at 39 °C for 24 h. Among the tested extracts, eight extracts decreased CH4 production by >20%, compared to the corresponding controls: stems of Vitex negundo var. incisa, stems of Amelanchier asiatica, fruit of Reynoutria sachalinensis, seeds of Tribulus terrestris, seeds of Pharbitis nil, leaves of Alnus japonica, stem and bark of Carpinus tschonoskii, and stems of Acer truncatum. A confirmation assay of the eight plant extracts at a dosage of 10 mg with four replications repeated on 3 different days revealed that the extracts decreased CH4 concentration in the total gas (7–15%) and total CH4 production (17–37%), compared to the control. This is the first report to identify the anti-methanogenic activities of eight potential plant extracts. All extracts decreased ammonia (NH3-N) concentrations. Negative effects on total gas and volatile fatty acid (VFA) production were also noted for all extracts that were rich in hydrolysable tannins and total saponins or fatty acids. The underlying modes of action differed among plants: extracts from P. nil, V. negundo var. incisa, A. asiatica, and R. sachalinensis resulted in a decrease in total methanogen or the protozoan population (p < 0.05) but extracts from other plants did not. Furthermore, extracts from P. nil decreased the population of total protozoa and increased the proportion of propionate among VFAs (p < 0.05). Identifying bioactive compounds in seeds of P. nil by gas chromatography-mass spectrometry analysis revealed enrichment of linoleic acid (18:2). Overall, seeds of P. nil could be a possible alternative to ionophores or oil seeds to mitigate ruminal CH4 production.


2006 ◽  
Vol 51 (1) ◽  
Author(s):  
Alicia Gómez-Barrio ◽  
David Montero-Pereira ◽  
Juan Nogal-Ruiz ◽  
José Escario ◽  
Susana Muelas-Serrano ◽  
...  

AbstractA study of some antiparasitic properties of several homoallylamines and related tetrahydroquinolines and quinolines, previously described, was carried out using in vitro activity assays against the epimastigote form of Trypanosoma cruzi and against Trichomonas vaginalis. Unspecific cytotoxicity against murine macrophages was also studied. Although the antichagasic and trichomonacidal activities are not comparable to those of the standard drugs, nifurtimox and metronidazole, some of the compounds exhibit an interesting specific antiparasitic activity.


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