Veränderungen von Sameninhaltsstoffen der Senfarten Sinapis alba, Brassica juncea und Brassica nigra unter verschiedenen Klimavarianten im Phytotron

1983 ◽  
Vol 85 (2) ◽  
pp. 77-86 ◽  
Author(s):  
R. Marquard
Phytomedicine ◽  
2018 ◽  
Vol 50 ◽  
pp. 196-204 ◽  
Author(s):  
Yan-Fang Xian ◽  
Zhen Hu ◽  
Siu-Po Ip ◽  
Jian-Nan Chen ◽  
Zi-Ren Su ◽  
...  

Biomolecules ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 489 ◽  
Author(s):  
L’Hocine ◽  
Pitre ◽  
Achouri

Currently, information on the allergens profiles of different mustard varieties is rather scarce. Therefore, the objective of this study was to assess protein profiles and immunoglobulin E (IgE)-binding patterns of selected Canadian mustard varieties. Optimization of a non-denaturing protein extraction from the seeds of selected mustard varieties was first undertaken, and the various extracts were quantitatively and qualitatively analyzed by means of protein recovery determination and protein profiling. The IgE-binding patterns of selected mustard seeds extracts were assessed by immunoblotting using sera from mustard sensitized and allergic individuals. In addition to the known mustard allergens—Sin a 2 (11S globulins), Sin a 1, and Bra j 1 (2S albumins)—the presence of other new IgE-binding protein bands was revealed from both Sinapis alba and Brassica juncea varieties. Mass spectrometry (MS) analysis of the in-gel digested IgE-reactive bands identified the unknown ones as being oleosin, β-glucosidase, enolase, and glutathione-S transferase proteins. A bioinformatic comparison of the amino acid sequence of the new IgE-binding mustard proteins with those of know allergens revealed a number of strong homologies that are highly relevant for potential allergic cross-reactivity. Moreover, it was found that Sin a 1, Bra j 1, and cruciferin polypeptides exhibited a stronger IgE reactivity under non-reducing conditions in comparison to reducing conditions, demonstrating the recognition of conformational epitopes. These results further support the utilization of non-denaturing extraction and analysis conditions, as denaturing conditions may lead to failure in the detection of important immunoreactive epitopes.


1996 ◽  
Vol 115 (6) ◽  
pp. 480-483 ◽  
Author(s):  
K. Gaikwad ◽  
P. B. Kirti ◽  
A. Sharma ◽  
S. Prakash ◽  
V. L. Chopra

HortScience ◽  
2017 ◽  
Vol 52 (3) ◽  
pp. 371-376 ◽  
Author(s):  
Xi Wang ◽  
Genhua Niu ◽  
Mengmeng Gu ◽  
Paul A. Baumann ◽  
Joseph Masabni

Mustard Seed Meals (MSMs) are by-products of biodiesel and an alternative to conventional herbicides for organic farming. However, MSMs might also suppress the emergence of vegetable seedlings. The objective of this study was to determine the response of vegetable seedling emergence to different MSM types and rates applied as an alternative herbicide. Six types of vegetable seeds, onion (Allium cepa), two cultivars of lettuce (Lactuca sativa ‘Black Seeded Simpson’ and ‘Buttercrunch’), mustard (Brassica juncea), kale (Brassica oleracea), and Mizuna (Brassica rapa var. japonica), were sowed in petri dishes containing germination mix. MSMs (Sinapis alba ‘IdaGold’ and B. juncea ‘Pacific Gold’) were incorporated into the germination mix at 0, 88, 176, or 265 g·m−2. Petri dishes were sealed for 1, 3, 5, or 7 days after sowing. For onion, ‘Pacific Gold’ had a greater suppressive effect on seedling emergence than ‘IdaGold’. For kale and mustard, ‘IdaGold’ and ‘Pacific Gold’ had similar suppressive effects on seedling emergence, but ‘Pacific Gold’ delayed emergence of kale at 88 g·m−2 when sealed for 3, 5, and 7 days. For Mizuna, ‘IdaGold’ had more suppressive effects than ‘Pacific Gold’ on seedling emergence, while sealing delayed but did not decrease emergence percentage (EP) at the lower rate (88 g·m−2) compared with the control treatment. For ‘Buttercrunch’ lettuce, there were no differences in the suppressive effects between the two MSMs. For ‘Black Seeded Simpson’ lettuce, ‘Pacific Gold’ had more suppressive effects on seedling emergence than ‘IdaGold’ when sealed at the lower rate (88 g·m−2) for longer durations (7 days) or at higher rates (176 and 265 g·m−2) for shorter durations (1 and 3 days). These results suggest that MSMs might suppress vegetable seedling emergence when applied at high rates (176 and 265 g·m−2), and sealing for more than 7 days after sowing may strengthen the suppressive effect. Extending sealing duration at the medium rates could achieve similar weed control results to high rates without sealing.


2008 ◽  
Vol 88 (2) ◽  
pp. 367-372 ◽  
Author(s):  
H. J. Beckie ◽  
E. N. Johnson ◽  
R. E. Blackshaw ◽  
Y. Gan

Competitive crops or cultivars can be an important component of integrated weed management systems. A study was conducted from 2003 to 2006 at four sites across semiarid prairie ecoregions in Saskatchewan and Alberta to investigate the productivity and quality of canola (Brassica napusL.) and mustard cultivars under weed competition. Four open-pollinated canola cultivars, four hybrid canola cultivars, two canola-quality mustard and two oriental mustard cultivars [Brassica juncea (L.) Czern. & Coss.], and two yellow mustard (Sinapis alba L.) cultivars were grown under weedy and weed-free conditions. When combined across site-years, crop aboveground biomass at maturity and seed yield were reduced by weed interference, except for yellow and oriental mustard. However, seed oil and protein content of cultivars were not affected by weed competition. Among crop types, yellow and oriental mustard were best able to maintain biomass and seed yield under weed interference, followed in decreasing order of competitiveness by hybrid and open-pollinated canola, then canola-quality mustard. Key words: Brassica napus, Brassica juncea, Sinapis alba, weed competition


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