Laser-induced thermal injury to dermal blood vessels: Analysis of wavelength (585 nm vs. 595 nm), cryogen spray cooling, and wound healing effects

The formation of new scaffolds to enhance healing magnitude is necessarily required in biomedical applications. Granulation tissue formation is a crucial stage of wound healing in which granulation tissue grows on the surface of a wound by the formation of connective tissue and blood vessels. In the present study, porous hydrogels were synthesized using chitosan incorporating latex of the Calotropis procera plant by using a freeze–thaw cycle to stimulate the formation of granulation tissue and angiogenesis in wound healing applications. Structural analysis through Fourier transform infrared (FTIR) spectroscopy confirmed the interaction between chitosan and Calotropis procera. Latex extract containing hydrogel showed slightly higher absorption than the control during water absorption analysis. Thermogravimetric analysis showed high thermal stability of the 60:40 combination of chitosan (CS) and Calotropis procera as compared to all other treatments and controls. A fabricated scaffold application on a chick chorioallantoic membrane (CAM) showed that all hydrogels containing latex extract resulted in a significant formation of blood vessels and regeneration of cells. Overall, the formation of connective tissues and blood capillaries and healing magnitude decreased in ascending order of concentration of extract.

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Radial and temporal variations in surface heat transfer during cryogen spray cooling

Physics in Medicine and Biology ◽

10.1088/0031-9155/50/2/015 ◽

2005 ◽

Vol 50 (2) ◽

pp. 387-397 ◽

Cited By ~ 28

Author(s):

Walfre Franco ◽

Jie Liu ◽

Guo-Xiang Wang ◽

J Stuart Nelson ◽

Guillermo Aguilar

Keyword(s):

Heat Transfer ◽

Spray Cooling ◽

Temporal Variations ◽

Surface Heat ◽

Surface Heat Transfer ◽

Cryogen Spray Cooling

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Dynamics of cryogen deposition relative to heat extraction rate during cryogen spray cooling

10.1117/12.386235 ◽

2000 ◽

Cited By ~ 27

Author(s):

Wim Verkruysse ◽

Boris Majaron ◽

Guillermo Aguilar ◽

Lars O. Svaasand ◽

J. Stuart Nelson

Keyword(s):

Spray Cooling ◽

Extraction Rate ◽

Heat Extraction ◽

Cryogen Spray Cooling ◽

Heat Extraction Rate

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Role of Leukocytes and Endothelial Cells in the Development of Angiogenesis in Inflammation and Wound Healing

Archives of Pathology & Laboratory Medicine ◽

10.5858/2001-125-0067-rolaec ◽

2001 ◽

Vol 125 (1) ◽

pp. 67-71 ◽

Cited By ~ 2

Author(s):

Mark W. Lingen

Keyword(s):

Wound Healing ◽

Endothelial Cells ◽

Blood Vessels ◽

Peripheral Blood ◽

Inflammatory Process ◽

Signs And Symptoms ◽

Critical Component ◽

Complex Environment ◽

Seminal Article

Abstract The basic signs and symptoms of inflammation and wound healing have been appreciated for thousands of years. However, the specific cells involved and their roles in this complex environment are still being elucidated today. In 1926, the origin of the phagocytic mononuclear ameboid wandering cell (macrophage) had not been determined. One popular theory was that the cells were differentiated from the endothelial cells of the nearby blood vessels, whereas others believed that the cells came from the peripheral blood or resting wandering cells. The purpose of this article is to review the seminal article published by Lang regarding this topic nearly 75 years ago. In addition, this article will review what is now known with regard to the role of the macrophage and endothelial cells in the development of angiogenesis, which is arguably the most critical component of successful inflammatory process or wound healing.

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