Characterization of Ultra-Thin Temperature-Responsive Polymer Layer and Its Polymer Thickness Dependency on Cell Attachment/Detachment Properties

2010 ◽  
Vol 10 (10) ◽  
pp. 1117-1129 ◽  
Author(s):  
Kazuhiro Fukumori ◽  
Yoshikatsu Akiyama ◽  
Yoshikazu Kumashiro ◽  
Jun Kobayashi ◽  
Masayuki Yamato ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Polymer Layer ◽  
Temperature Responsive ◽  
Responsive Polymer ◽  
Polymer Thickness

scholarly journals Characterization of Lassa Virus Cell Entry and Neutralization with Lassa Virus Pseudoparticles

2009 ◽  
Vol 83 (7) ◽  
pp. 3228-3237 ◽  
Author(s):  
François-Loic Cosset ◽  
Philippe Marianneau ◽  
Geraldine Verney ◽  
Fabrice Gallais ◽  
Noel Tordo ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Neutralizing Antibodies ◽  
Cell Attachment ◽  
Low Ph ◽  
Cell Entry ◽  
Lassa Virus ◽  
Ph Optimum ◽  
Humoral Immune

ABSTRACT The cell entry and humoral immune response of the human pathogen Lassa virus (LV), a biosafety level 4 (BSL4) Old World arenavirus, are not well characterized. LV pseudoparticles (LVpp) are a surrogate model system that has been used to decipher factors and routes involved in LV cell entry under BSL2 conditions. Here, we describe LVpp, which are highly infectious, with titers approaching those obtained with pseudoparticles displaying G protein of vesicular stomatitis virus and their the use for the characterization of LV cell entry and neutralization. Upon cell attachment, LVpp utilize endocytic vesicles for cell entry as described for many pH-dependent viruses. However, the fusion of the LV glycoproteins is activated at unusually low pH values, with optimal fusion occurring between pH 4.5 and 3, a pH range at which fusion characteristics of viral glycoproteins have so far remained largely unexplored. Consistent with a shifted pH optimum for fusion activation, we found wild-type LV and LVpp to display a remarkable resistance to exposure to low pH. Finally, LVpp allow the fast and quantifiable detection of neutralizing antibodies in human and animal sera and will thus facilitate the study of the humoral immune response in LV infections.

Cell Attachment–Detachment Control on Temperature-Responsive Thin Surfaces for Novel Tissue Engineering

2010 ◽  
Vol 38 (6) ◽  
pp. 1977-1988 ◽  
Author(s):  
Yoshikazu Kumashiro ◽  
Masayuki Yamato ◽  
Teruo Okano
Keyword(s):  
Tissue Engineering ◽  
Cell Attachment ◽  
Temperature Responsive

Photo-Cross-Linkable PNIPAAm Copolymers. 1. Synthesis and Characterization of Constrained Temperature-Responsive Hydrogel Layers

2002 ◽  
Vol 35 (16) ◽  
pp. 6377-6383 ◽  
Author(s):  
Dirk Kuckling ◽  
Marianne E. Harmon ◽  
Curtis W. Frank
Keyword(s):  
Synthesis And Characterization ◽  
Temperature Responsive

Temperature-responsive polymer–gold nanocomposites as intelligent therapeutic systems

2007 ◽  
Vol 83A (3) ◽  
pp. 692-695 ◽  
Author(s):  
Donald E. Owens ◽  
Jackson K. Eby ◽  
Yicun Jian ◽  
Nicholas A. Peppas
Keyword(s):  
Temperature Responsive ◽  
Responsive Polymer ◽  
Gold Nanocomposites

scholarly journals Biological characterization of the modified poly(dimethylsiloxane) surfaces based on cell attachment and toxicity assays

2018 ◽  
Vol 12 (4) ◽  
pp. 044105 ◽  
Author(s):  
Elzbieta Jastrzebska ◽  
Agnieszka Zuchowska ◽  
Sylwia Flis ◽  
Patrycja Sokolowska ◽  
Magdalena Bulka ◽  
...  
Keyword(s):  
Cell Attachment ◽  
Biological Characterization

scholarly journals Preparation and Characterization of Temperature-responsive Affinity Chromatographic Stationary Phase Modified with Heparin

2012 ◽  
Vol 70 (05) ◽  
pp. 561
Author(s):  
Xiao Liang ◽  
Zongjian Liu ◽  
Rongji Dai ◽  
Wei Wang ◽  
Fangfang Geng ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Temperature Responsive
Sign in / Sign up

Export Citation Format

Share Document