scholarly journals Preparation and Characterization of Temperature-responsive Affinity Chromatographic Stationary Phase Modified with Heparin

2012 ◽  
Vol 70 (05) ◽  
pp. 561
Author(s):  
Xiao Liang ◽  
Zongjian Liu ◽  
Rongji Dai ◽  
Wei Wang ◽  
Fangfang Geng ◽  
...  
Author(s):  
B. L. Soloff ◽  
T. A. Rado

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.


2009 ◽  
Vol 27 (11) ◽  
pp. 2229-2236 ◽  
Author(s):  
Rongyue Zhang ◽  
Li Qi ◽  
Qin Xiao ◽  
Peiyong Xin ◽  
Gengliang Yang

2012 ◽  
Vol 23 (11) ◽  
pp. 1259-1262 ◽  
Author(s):  
Guo Wen Chen ◽  
Chen Zhang ◽  
Wen Jie Li ◽  
Chuan Jian Zhou ◽  
Sheng Yu Feng

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