Mouse lumbar and cervical spinal cord blood flow measurements by arterial spin labeling: Sensitivity optimization and first application

2009 ◽  
Vol 62 (2) ◽  
pp. 430-439 ◽  
Author(s):  
Guillaume Duhamel ◽  
Virginie Callot ◽  
Patrick Decherchi ◽  
Yann Le Fur ◽  
Tanguy Marqueste ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Blood Flow ◽  
Cord Blood ◽  
Arterial Spin Labeling ◽  
Cervical Spinal Cord ◽  
Spin Labeling ◽  
Spinal Cord Blood Flow ◽  
Flow Measurements ◽  
Blood Flow Measurements

Spinal cord blood flow measurement by arterial spin labeling

2008 ◽  
Vol 59 (4) ◽  
pp. 846-854 ◽  
Author(s):  
Guillaume Duhamel ◽  
Virginie Callot ◽  
Patrick J. Cozzone ◽  
Frank Kober
Keyword(s):  
Spinal Cord ◽  
Blood Flow ◽  
Cord Blood ◽  
Arterial Spin Labeling ◽  
Flow Measurement ◽  
Spin Labeling ◽  
Blood Flow Measurement ◽  
Spinal Cord Blood Flow

Spinal Cord Blood Flow in Experimental Transient Traumatic Paraplegia

1980 ◽  
Vol 52 (3) ◽  
pp. 335-345 ◽  
Author(s):  
Dean C. Lohse ◽  
Howard J. Senter ◽  
John S. Kauer ◽  
Richard Wohns
Keyword(s):  
Spinal Cord ◽  
Blood Flow ◽  
White Matter ◽  
Cord Blood ◽  
Spinal Cord Blood Flow ◽  
Flow Measurements ◽  
Content Type ◽  
The Mean ◽  
Blood Flow Measurements ◽  
Fine Print

✓ Blood flow in the lateral funiculus of the thoracic spinal cord was measured in 24 anesthetized cats using the hydrogen clearance method. In a control series of eight nontraumatized animals, blood flow measurements were taken from the T-5 and T-6 segments for 6 consecutive hours. The mean spinal cord blood flow (SCBF) in the control group was 12.8 ± 3.51 (SD) ml/min/100 gm on the basis of 107 measurements over 6 hours. In the experimental groups, 16 animals were similarly prepared. The spinal cords of these animals were then traumatized by dropping a 20-gm weight 5 cm (100 gm-cm trauma) or 13 cm (260 gm-cm trauma) onto the T-5 segment. Previous experiments have shown that these trauma levels lead to a transient paraplegia of less than 10 and 30 days' duration, respectively. Two hundred blood flow measurements from T-5 and T-6 were taken over the 6 hours following trauma. In the seven animals of the 100 gm-cm group, mean SCBF after trauma from the T-5 segment was 12.6 ± 3.45 (SD) ml/min/100 gm on the basis of 50 measurements taken over 6 hours; not significantly different from the controls (p > 0.70). In the 260 gm-cm group, mean SCBF from T-5 for 6 hours after trauma was 17.3 ± 6.60 (SD) ml/min/100 gm; significantly higher than controls (p < 0.001). Mean SCBF 3 to 6 hours after trauma was significantly elevated over controls (p < 0.05). The mean hyperemia in the 260 gm-cm group was found to be due to marked hyperemia in only four animals of the series, while five animals maintained blood flows in the normal range. This experiment provides quantitative evidence that white matter ischemia does not occur in spinal cord injuries that can be expected to produce only transient paraplegia. The data support the concept that white matter ischemia in the acute phase of severe spinal cord trauma may be related to secondary injury and subsequent permanent paraplegia.

Regional spinal cord blood flow measurements (r.S.C.B.F.) in spinal cord acute compression caused by an epidural balloon

1987 ◽  
Vol 88 (3-4) ◽  
pp. 135-141 ◽  
Author(s):  
G. Karoutas ◽  
Ph. Tsitsopoulos ◽  
N. Taskos ◽  
D. Karacostas ◽  
Th. Tzotzoras ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Blood Flow ◽  
Cord Blood ◽  
Spinal Cord Blood Flow ◽  
Flow Measurements ◽  
Blood Flow Measurements

scholarly journals Fiber-optic Monitoring of Spinal Cord Hemodynamics in Experimental Aortic Occlusion

2015 ◽  
Vol 123 (6) ◽  
pp. 1362-1373 ◽  
Author(s):  
Angela S. Kogler ◽  
Thomas V. Bilfinger ◽  
Robert M. Galler ◽  
Rickson C. Mesquita ◽  
Michael Cutrone ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Blood Flow ◽  
Cord Blood ◽  
Fiber Optic ◽  
Spinal Cord Ischemia ◽  
Correlation Spectroscopy ◽  
Aortic Occlusion ◽  
Spinal Cord Blood Flow ◽  
Flow Measurements ◽  
Blood Flow Measurements

Abstract Background Spinal cord ischemia occurs frequently during thoracic aneurysm repair. Current methods based on electrophysiology techniques to detect ischemia are indirect, non-specific, and temporally slow. In this article, the authors report the testing of a spinal cord blood flow and oxygenation monitor, based on diffuse correlation and optical spectroscopies, during aortic occlusion in a sheep model. Methods Testing was carried out in 16 Dorset sheep. Sensitivity in detecting spinal cord blood flow and oxygenation changes during aortic occlusion, pharmacologically induced hypotension and hypertension, and physiologically induced hypoxia/hypercarbia was assessed. Accuracy of the diffuse correlation spectroscopy measurements was determined via comparison with microsphere blood flow measurements. Precision was assessed through repeated measurements in response to pharmacologic interventions. Results The fiber-optic probe can be placed percutaneously and is capable of continuously measuring spinal cord blood flow and oxygenation preoperatively, intraoperatively, and postoperatively. The device is sensitive to spinal cord blood flow and oxygenation changes associated with aortic occlusion, immediately detecting a decrease in blood flow (−65 ± 32%; n = 32) and blood oxygenation (−17 ± 13%, n = 11) in 100% of trials. Comparison of spinal cord blood flow measurements by the device with microsphere measurements led to a correlation of R2 = 0.49, P &lt; 0.01, and the within-sheep coefficient of variation was 9.69%. Finally, diffuse correlation spectroscopy is temporally more sensitive to ischemic interventions than motor-evoked potentials. Conclusion The first-generation spinal fiber-optic monitoring device offers a novel and potentially important step forward in the monitoring of spinal cord ischemia.

Validation of laser-Doppler flowmetry in measurement of spinal cord blood flow

1989 ◽  
Vol 257 (2) ◽  
pp. H674-H680 ◽  
Author(s):  
P. J. Lindsberg ◽  
J. T. O'Neill ◽  
I. A. Paakkari ◽  
J. M. Hallenbeck ◽  
G. Feuerstein
Keyword(s):  
Spinal Cord ◽  
Blood Flow ◽  
Cord Blood ◽  
Laser Doppler Flowmetry ◽  
Laser Doppler ◽  
Microvascular Blood Flow ◽  
Base Line ◽  
Spinal Cord Blood Flow ◽  
Flow Measurements ◽  
Doppler Flowmetry

Laser-Doppler flowmetry (LDF) is a non-invasive method for continuous on-line monitoring of microvascular blood flow. LDF has previously been validated with established methods in various tissues, yet its validity and resolution in the central nervous system (CNS) remain unclear. We compared LDF with the microsphere method (MS) using two independent laser probes placed on the dorsal lumbar spinal cord (L5 laminectomy) of anesthetized rabbits (n = 9). After base-line flow measurements, spinal cord blood flow (SCBF) was increased (up to 50%) with phenylephrine (10-80 micrograms.kg-1.min-1 iv) and decreased (up to 50%) with chlorisondamine (10 mg/kg iv) or other stimuli. The percentage changes of lumbar SCBF and vascular resistance (VR) from the base line obtained by LDF and MS excellently agreed (rBF = 0.86, rVR = 0.94, P less than 0.0001). LDF estimated also the absolute SCBF values parallel to MS (r = 0.77, P less than 0.001). In conclusion, the validity of LDF in estimating the SCBF and dynamic changes of BF and VR is confirmed. Therefore, LDF may prove useful for monitoring CNS microcirculation in normal or pathophysiological states.

scholarly journals Accuracy of Parenchymal Cerebral Blood Flow Measurements Using Pseudocontinuous Arterial Spin-Labeling in Healthy Volunteers

2015 ◽  
Vol 36 (10) ◽  
pp. 1816-1821 ◽  
Author(s):  
K. Ambarki ◽  
A. Wåhlin ◽  
L. Zarrinkoob ◽  
R. Wirestam ◽  
J. Petr ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Healthy Volunteers ◽  
Arterial Spin Labeling ◽  
Spin Labeling ◽  
Flow Measurements ◽  
Blood Flow Measurements

Cerebral blood flow measurements in infants using look-locker arterial spin labeling

2014 ◽  
Vol 41 (6) ◽  
pp. 1591-1600 ◽  
Author(s):  
Marta Varela ◽  
Esben T. Petersen ◽  
Xavier Golay ◽  
Joseph V. Hajnal
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Arterial Spin Labeling ◽  
Spin Labeling ◽  
Flow Measurements ◽  
Blood Flow Measurements
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