Rapid injection cystometry and hydroxyproline content in the acutely overdistended rabbit bladder

1992 ◽  
Vol 11 (1) ◽  
pp. 33-39
Author(s):  
Hayoung Kim ◽  
William D. Belville ◽  
Gary Wedemeyer ◽  
Edward J. McGuire
Keyword(s):  
Hydroxyproline Content ◽  
Rapid Injection ◽  
Rabbit Bladder

Microvasculature and structure of hemal lymph nodes in the wall of the rabbit bladder: a vascular corrosion casting and EM study

1995 ◽  
Vol 53 ◽  
pp. 1074-1075
Author(s):  
F.E. Hossler ◽  
M.I. McKamey ◽  
F.C. Monson
Keyword(s):  
Lymph Nodes ◽  
Light Microscopy ◽  
Corrosion Casting ◽  
Fixed Tissue ◽  
Infusion Pressure ◽  
India Ink ◽  
Cacodylate Buffer ◽  
Rabbit Bladder ◽  
Lateral Walls ◽  
Comprehensive Study

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

Sensory Quality, Textural Characteristics and Hydroxyproline Content of Irradiated Beefsteaks

1998 ◽  
Author(s):  
Gary W. Shults ◽  
Joseph S. Cohen ◽  
Eugen Wierbicki ◽  
Vera C. Mason
Keyword(s):  
Sensory Quality ◽  
Hydroxyproline Content ◽  
Textural Characteristics

Tetrodotoxin-resistant release of ATP from superfused rabbit detrusor muscle during electrical field stimulation in the presence of luciferin–luciferase

1984 ◽  
Vol 62 (1) ◽  
pp. 153-156 ◽  
Author(s):  
Archana Chaudhry ◽  
John W. Downie ◽  
Thomas D. White
Keyword(s):  
Action Potentials ◽  
Electrical Field Stimulation ◽  
Detrusor Muscle ◽  
Electrical Field ◽  
Stimulation Parameters ◽  
Rabbit Bladder ◽  
Firefly Luciferin ◽  
Rabbit Urinary Bladder ◽  
Stimulation Of

The present study was carried out to assess the possible role of ATP in the noncholinergic, nonadrenergic transmission in the rabbit urinary bladder. When rabbit detrusor muscle strips were superfused with medium containing firefly luciferin–luciferase and stimulated transmurally at low stimulation parameters, tetrodotoxin-sensitive contractions were obtained but no release of ATP could be detected. However, at somewhat higher stimulation parameters, release of ATP was observed. This release of ATP was not diminished by tetrodotoxin indicating that ATP was not likely released as a result of propagated action potentials in nerves. Because contractions persisted in the presence of tetrodotoxin, it is possible that the ATP might have been released as a result of direct electrical stimulation of the muscle. These results do not support the idea that ATP is released as a neurotransmitter in the rabbit bladder.

Effects of Shielded or Unshielded Laser and Electrohydraulic Lithotripsy on Rabbit Bladder

1990 ◽  
Vol 143 (4) ◽  
pp. 857-860 ◽  
Author(s):  
Krishna M. Bhatta ◽  
David I. Rosen ◽  
Thomas J. Flotte ◽  
Stephen P. Dretler ◽  
Norman S. Nishioka
Keyword(s):  
Electrohydraulic Lithotripsy ◽  
Rabbit Bladder

scholarly journals Nitrotyrosine Density of Rabbit Urinary Bladder Muscle and Mucosa Measured via Western Blotting and 96-Well Plate Analysis

ISRN Urology ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Brittany Fitzpatrick ◽  
Catherine Schuler ◽  
Robert E. Leggett ◽  
Robert M. Levin
Keyword(s):  
Quantitative Analysis ◽  
Western Blotting ◽  
Optical Density ◽  
Tissue Concentration ◽  
Detrusor Muscle ◽  
Pvdf Membrane ◽  
Sds Page ◽  
Wash Buffer ◽  
Rabbit Bladder ◽  
Plate Analysis

Purpose. Nitrotyrosine was quantitated in rabbit bladder muscle and mucosa using two analytical systems: Western blotting analyses and a 96-well plate quantitative analysis kit. Materials and Methods. Rabbit bladder muscle and mucosa were obtained from control rabbits. For the Western analysis, the samples were loaded into a SDS page gel and then transferred to a PVDF membrane. The optical density was measured using a Kodak Scanner. Using the 96-well plate, the samples and standards were loaded, incubated with primary and secondary antibody, washed and vacuumed with 10x wash buffer three times between each incubation period. Stop buffer was added to the plate and the results were quantified via the plate reader. Results. For both muscle and mucosa tissue, the optical density readings were linear with tissue concentration; the concentration of nitrotyrosine in the mucosa was significantly higher than in the muscle. However, whereas the Western blot analysis is based on relative optical densities, the 96-well plate kit provides a truly quantitative analysis. Discussion. Mucosa tissue displayed a higher density of nitrotyrosine than did detrusor muscle tissue. This may well be due to the significantly higher metabolic activity of the mucosa compared to the muscle.

Effect of Magnesium on the Contraction of Whole Rabbit Bladder in vitro

1996 ◽  
Vol 57 (3) ◽  
pp. 141-144 ◽  
Author(s):  
Masahiko Saito ◽  
Soichiro Hasegawa ◽  
Kumiko Kato ◽  
Atsuo Kondo
Keyword(s):  
Rabbit Bladder

The Cholinergic and Purinergic Components of Detrusor Contractility in a Whole Rabbit Bladder Model

1992 ◽  
Vol 148 (3 Part 1) ◽  
pp. 906-909 ◽  
Author(s):  
Michael B. Chancellor ◽  
Steven A. Kaplan ◽  
Jerry G. Blaivas
Keyword(s):  
Detrusor Contractility ◽  
Rabbit Bladder ◽  
Bladder Model

Systemic administration of naked plasmid encoding HGF attenuates puromycin aminonucleoside-induced damage of murine glomerular podocytes

2011 ◽  
Vol 301 (4) ◽  
pp. F784-F792 ◽  
Author(s):  
Xuan Bu ◽  
Yang Zhou ◽  
Hua Zhang ◽  
Wenjing Qiu ◽  
Lu Chen ◽  
...  
Keyword(s):  
Wilms Tumor ◽  
Gene Transfection ◽  
Systemic Administration ◽  
Renal Diseases ◽  
Puromycin Aminonucleoside ◽  
Podocyte Injury ◽  
Rapid Injection ◽  
Naked Plasmid

Podocyte injury is considered to play important roles in the pathogenesis of human glomerular disease. There is accumulating evidence suggesting that hepatocyte growth factor (HGF) elicits preventive activity for glomerular cells in animal models of chronic renal diseases. In this study, we demonstrated that delivery of a naked plasmid vector encoding the human HGF gene into mice by a hydrodynamic-based in vivo gene transfection approach markedly reduced proteinuria and attenuated podocyte injury in a mouse model induced by puromycin aminonucleoside (PAN) injection. Systemic administration by rapid injection via the tail vein of a naked plasmid containing HGF cDNA driven under a cytomegalovirus promoter (pCMV-HGF) produced a remarkable level of human HGF protein in the circulation. Tissue distribution studies suggested that the kidney expressed a high level of the HGF transgene. Meanwhile, compared with tubules and interstitium, a higher level of exogenous HGF protein was detected in the glomeruli. Administration of pCMV-HGF dramatically abated the urine albumin excretion and podocyte injury in PAN nephropathy in mice. Exogenous expression of HGF produced evidently beneficial effects, leading to restoration of Wilms' tumor-1 (WT1) and α-actinin-4 expression and attenuation of ultrastructural damage of the podocytes. In vitro, HGF not only restored WT1 and α-actinin-4 expression but also inhibited albumin leakage of podocytes incubated with PAN in a Transwell culture chamber. These results suggest that HGF might provide a novel strategy for amelioration of podocyte injury.

Attenuation of bleomycin-induced pneumopathy in mice by a caspase inhibitor

2001 ◽  
Vol 280 (2) ◽  
pp. L316-L325 ◽  
Author(s):  
Kazuyoshi Kuwano ◽  
Ritsuko Kunitake ◽  
Takashige Maeyama ◽  
Naoki Hagimoto ◽  
Masayuki Kawasaki ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Lung Inflammation ◽  
Caspase 3 ◽  
Fas Ligand ◽  
Inflammatory Cells ◽  
Lung Epithelial Cells ◽  
Caspase Inhibitor ◽  
Hydroxyproline Content ◽  
Caspase 1 ◽  
Lung Epithelial

Caspases have been implicated in the effector process of apoptosis in several systems including the Fas-Fas ligand pathway. We previously demonstrated that excessive apoptosis of lung epithelial cells and the Fas-Fas ligand pathway were essential in the pathogenesis of bleomycin-induced pneumopathy in mice. Therefore, the purpose of this study was to investigate whether a caspase inhibitor could prevent the development of this model. The expression of caspase-1 and caspase-3 was upregulated on lung epithelial cells, alveolar macrophages, and infiltrating inflammatory cells in this model. We demonstrated that a broad-spectrum caspase inhibitor, N-benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone, decreased the caspase-1- and caspase-3-like activity, the number of apoptotic cells, the pathological grade of lung inflammation and fibrosis, and the hydroxyproline content in lung tissues in this model. We conclude that caspase inhibitors could be a new therapeutic approach against lung injury and pulmonary fibrosis.

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