Effects of Shielded or Unshielded Laser and Electrohydraulic Lithotripsy on Rabbit Bladder

1990 ◽  
Vol 143 (4) ◽  
pp. 857-860 ◽  
Author(s):  
Krishna M. Bhatta ◽  
David I. Rosen ◽  
Thomas J. Flotte ◽  
Stephen P. Dretler ◽  
Norman S. Nishioka
Keyword(s):  
Electrohydraulic Lithotripsy ◽  
Rabbit Bladder

Microvasculature and structure of hemal lymph nodes in the wall of the rabbit bladder: a vascular corrosion casting and EM study

1995 ◽  
Vol 53 ◽  
pp. 1074-1075
Author(s):  
F.E. Hossler ◽  
M.I. McKamey ◽  
F.C. Monson
Keyword(s):  
Lymph Nodes ◽  
Light Microscopy ◽  
Corrosion Casting ◽  
Fixed Tissue ◽  
Infusion Pressure ◽  
India Ink ◽  
Cacodylate Buffer ◽  
Rabbit Bladder ◽  
Lateral Walls ◽  
Comprehensive Study

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

Bouveret’s Syndrome – Case Series with Therapeutic Implications

2020 ◽  
Vol 58 (04) ◽  
pp. 352-356
Author(s):  
Tobias Kukiolka ◽  
Jan Borovicka ◽  
Stephan Baumeler ◽  
Marc Schiesser ◽  
Christoph Gubler
Keyword(s):  
Balloon Dilatation ◽  
Rare Complication ◽  
Gallstone Disease ◽  
Treatment Algorithm ◽  
Case Series ◽  
Endoscopic Technique ◽  
Bouveret’S Syndrome ◽  
Therapeutic Implications ◽  
Electrohydraulic Lithotripsy ◽  
Bouveret's Syndrome

AbstractBouveret’s syndrome is a rare complication resulting from gallstone disease. Both surgical and endoscopical procedures are performed, with the disease to be seen as strictly interdisciplinary. There are no well-established recommendations for this condition. In this paper, we want to describe our experience from 6 cases in 3 Swiss hospitals from 2015 to 2017 with emphasis on the endoscopic technique of electrohydraulic lithotripsy followed by balloon dilatation and propose a treatment algorithm.

Tetrodotoxin-resistant release of ATP from superfused rabbit detrusor muscle during electrical field stimulation in the presence of luciferin–luciferase

1984 ◽  
Vol 62 (1) ◽  
pp. 153-156 ◽  
Author(s):  
Archana Chaudhry ◽  
John W. Downie ◽  
Thomas D. White
Keyword(s):  
Action Potentials ◽  
Electrical Field Stimulation ◽  
Detrusor Muscle ◽  
Electrical Field ◽  
Stimulation Parameters ◽  
Rabbit Bladder ◽  
Firefly Luciferin ◽  
Rabbit Urinary Bladder ◽  
Stimulation Of

The present study was carried out to assess the possible role of ATP in the noncholinergic, nonadrenergic transmission in the rabbit urinary bladder. When rabbit detrusor muscle strips were superfused with medium containing firefly luciferin–luciferase and stimulated transmurally at low stimulation parameters, tetrodotoxin-sensitive contractions were obtained but no release of ATP could be detected. However, at somewhat higher stimulation parameters, release of ATP was observed. This release of ATP was not diminished by tetrodotoxin indicating that ATP was not likely released as a result of propagated action potentials in nerves. Because contractions persisted in the presence of tetrodotoxin, it is possible that the ATP might have been released as a result of direct electrical stimulation of the muscle. These results do not support the idea that ATP is released as a neurotransmitter in the rabbit bladder.

Rapid injection cystometry and hydroxyproline content in the acutely overdistended rabbit bladder

1992 ◽  
Vol 11 (1) ◽  
pp. 33-39
Author(s):  
Hayoung Kim ◽  
William D. Belville ◽  
Gary Wedemeyer ◽  
Edward J. McGuire
Keyword(s):  
Hydroxyproline Content ◽  
Rapid Injection ◽  
Rabbit Bladder

scholarly journals Efficacy of spyglass-guided electrohydraulic lithotripsy in difficult bile duct stones

2014 ◽  
Vol 20 (6) ◽  
pp. 366 ◽  
Author(s):  
AbdulrahmanM Aljebreen ◽  
OthmanR Alharbi ◽  
Nahla Azzam ◽  
MajidA Almadi
Keyword(s):  
Bile Duct ◽  
Bile Duct Stones ◽  
Electrohydraulic Lithotripsy

scholarly journals Nitrotyrosine Density of Rabbit Urinary Bladder Muscle and Mucosa Measured via Western Blotting and 96-Well Plate Analysis

ISRN Urology ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Brittany Fitzpatrick ◽  
Catherine Schuler ◽  
Robert E. Leggett ◽  
Robert M. Levin
Keyword(s):  
Quantitative Analysis ◽  
Western Blotting ◽  
Optical Density ◽  
Tissue Concentration ◽  
Detrusor Muscle ◽  
Pvdf Membrane ◽  
Sds Page ◽  
Wash Buffer ◽  
Rabbit Bladder ◽  
Plate Analysis

Purpose. Nitrotyrosine was quantitated in rabbit bladder muscle and mucosa using two analytical systems: Western blotting analyses and a 96-well plate quantitative analysis kit. Materials and Methods. Rabbit bladder muscle and mucosa were obtained from control rabbits. For the Western analysis, the samples were loaded into a SDS page gel and then transferred to a PVDF membrane. The optical density was measured using a Kodak Scanner. Using the 96-well plate, the samples and standards were loaded, incubated with primary and secondary antibody, washed and vacuumed with 10x wash buffer three times between each incubation period. Stop buffer was added to the plate and the results were quantified via the plate reader. Results. For both muscle and mucosa tissue, the optical density readings were linear with tissue concentration; the concentration of nitrotyrosine in the mucosa was significantly higher than in the muscle. However, whereas the Western blot analysis is based on relative optical densities, the 96-well plate kit provides a truly quantitative analysis. Discussion. Mucosa tissue displayed a higher density of nitrotyrosine than did detrusor muscle tissue. This may well be due to the significantly higher metabolic activity of the mucosa compared to the muscle.

Effect of Magnesium on the Contraction of Whole Rabbit Bladder in vitro

1996 ◽  
Vol 57 (3) ◽  
pp. 141-144 ◽  
Author(s):  
Masahiko Saito ◽  
Soichiro Hasegawa ◽  
Kumiko Kato ◽  
Atsuo Kondo
Keyword(s):  
Rabbit Bladder
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