Microvasculature and structure of hemal lymph nodes in the wall of the rabbit bladder: a vascular corrosion casting and EM study

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

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A study of the microvasculature of the normal rabbit bladder with vascular corrosion casting, SEM and TEM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010014703x ◽

1993 ◽

Vol 51 ◽

pp. 238-239

Author(s):

Fred E. Hossler ◽

Frederick C. Monson

Keyword(s):

Bladder Wall ◽

Vascular Anatomy ◽

Bladder Function ◽

Corrosion Casting ◽

Mucosal Regeneration ◽

Thin Sections ◽

Cacodylate Buffer ◽

Rabbit Bladder ◽

Urine Storage ◽

Surgically Induced

The mammalian urinary bladder performs two functions, urine storage and expulsion. Bladder function is dependent upon the delivery of oxygen and nutrients via a rich blood supply, yet studies of the bladder vasculature during distension have concluded that blood flow is restricted during filling. Following surgically induced ischemia, regeneration of the wall and its vasculature have been reported but not described in detail. Beyond the gross level, the functional vascular anatomy of the bladder wall is poorly understood. Preliminary to studies of angiogenesis and mucosal regeneration following ischemia, and vascular accommodation during distension, the present study utilizes routine transmission (TEM) and scanning (SEM) electron microscopy, and vascular corrosion casting (VCC) to describe the normal microvasculature of the rabbit bladder.Bladders were perfuse fixed with 2% glutaraldehyde in 0.1 M cacodylate buffer, pH 7.3, for routine TEM and SEM. Thin sections were cut from Araldyte and stained with lead and uranium for TEM, and samples were critical point dried from ethanol and CO2 for SEM.

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Structure and blood supply of intrinsic lymph nodes in the wall of the rabbit urinary bladder—studies with light microscopy, electron microscopy, and vascular corrosion casting

The Anatomical Record ◽

10.1002/(sici)1097-0185(199811)252:3<477::aid-ar16>3.0.co;2-h ◽

1998 ◽

Vol 252 (3) ◽

pp. 477-484 ◽

Cited By ~ 7

Author(s):

Fred E. Hossler ◽

Frederick C. Monson

Keyword(s):

Electron Microscopy ◽

Urinary Bladder ◽

Lymph Nodes ◽

Light Microscopy ◽

Blood Supply ◽

Corrosion Casting ◽

Rabbit Urinary Bladder

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Hexachlorobenzene toxicity in the monkey ovary: III. Ultrastructure induced by high (10 mg/kg) dose exposure

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100084958 ◽

1991 ◽

Vol 49 ◽

pp. 130-131

Author(s):

A. Singh ◽

A. Dykeman ◽

J. Jarrelf ◽

D. C. Villeneuve

Keyword(s):

Present Report ◽

Reproductive Toxicity ◽

Control Group ◽

Primate Model ◽

Human Follicular Fluid ◽

Thin Sections ◽

Cynomolgus Monkeys ◽

Cacodylate Buffer ◽

Induction Cycle ◽

Comprehensive Study

Hexachlorobenzene (HCB), a persistent and mobile organochlorine pesticide, occurs in environment. HCB has been shown to be present in human follicular fluid. An objective of the present report, which is part of a comprehensive study on reproductive toxicity of HCB, was to determine the cytologic effects of the compound on ovarian follicles in a primate model.Materials and Methods. Eight Cynomolgus monkeys were housed under controlled conditions at Animal facility of Health and Welfare, Ottawa. Animals were orally administered gelatin capsules containing HCB mixed with glucose in daily dosages of 0.0 or 10 mg/kg b.w. for 90 days; the former was the control group. On the menstrual period following completion of dosing, the monkeys underwent an induction cycle of superovulation. At necropsy, one-half of an ovary from each animal was diced into ca. 2- to 3-mm cubed specimens that were fixed by immersion in 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.3). Subsequent procedures followed to obtain thin sections that were examined in a Hitachi H-7000 electron microscope have been described earlier.

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A Sarcocystis-like Protozoon in a Sheep with Lymphadenopathy and Myocarditis

Veterinary Pathology ◽

10.1177/030098587801500205 ◽

1978 ◽

Vol 15 (2) ◽

pp. 186-195 ◽

Cited By ~ 2

Author(s):

T. Landsverk ◽

H. Gamlem ◽

R. Svenkerud

Keyword(s):

Electron Microscopy ◽

Endothelial Cells ◽

Lymph Node ◽

Lymph Nodes ◽

Light Microscopy ◽

Vascular Endothelial Cells ◽

Protozoan Parasite ◽

Capillary Endothelium ◽

Vascular Endothelial ◽

Generalized Lymphadenopathy

A generalized enlargement of the lymph nodes was found in an emaciated adult ewe. Additional autopsy findings included tiny grey-white necrotic foci in the heart muscle, aspiration pneumonia and diffuse pleuritis. Light microscopy showed a generalized lymphadenopathy with Perilymphadenitis, depletion of lymphocytes and histiocytosis of the lymph node. In histiocytes and vascular endothelial cells of lymph nodes, septal capillary endothelium of lungs and capillary endothelium of myocardium, early stages of a protozoan parasite were found. In the myocardium, there were many foci of necrosis, some of which contained young cysts in the periphery. These cysts were morphologically similar to those of Sarcocystis. Electron microscopy of the early protozoan stages yielded evidence of schizogony and formation of merozoites.

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Microvascular Architecture of the Mouse Urinary Bladder Described with Vascular Corrosion Casting, Light Microscopy, SEM, and TEM

Microscopy and Microanalysis ◽

10.1017/s143192760909206x ◽

2009 ◽

Vol 15 (S2) ◽

pp. 984-985 ◽

Cited By ~ 1

Author(s):

F Hossler ◽

A Lametschwandtner ◽

R Kao ◽

C Bills ◽

F Finsterbusch

Keyword(s):

Urinary Bladder ◽

Light Microscopy ◽

Corrosion Casting ◽

Sem And Tem ◽

Microvascular Architecture

Extended abstract of a paper presented at Microscopy and Microanalysis 2009 in Richmond, Virginia, USA, July 26 – July 30, 2009

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Seed morphology of perennial taxa of Euphorbia section Pithyusa (Euphorbiaceae) in Turkey

Phytotaxa ◽

10.11646/phytotaxa.336.3.4 ◽

2018 ◽

Vol 336 (3) ◽

pp. 263

Author(s):

İLKER GENÇ ◽

ŞÜKRAN KÜLTÜR

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Light Microscopy ◽

Seed Morphology ◽

Seed Shape ◽

Seed Surface ◽

Surface Ornamentation ◽

Seed Characteristics ◽

Scanning Electron ◽

Comprehensive Study

A comprehensive study based on seed morphology of perennial Euphorbia (Euphorbiaceae) sect. Pithyusa species occurring in Turkey is presented. A total of 14 species were studied. Seed characteristics were examined using scanning electron microscopy (SEM) as well as dissecting light microscopy. Significant features are: seed size, seed shape, shape of caruncle and seed surface ornamentation. Three different seed surface types (smooth, pitted, and wrinkled) were observed. Four main seed shapes (ovoid, oblong, quadrangular, and globose), as well as seven types of seed coat ornamentation (reticulate-areolate, areolate, alveolate, falsifoveate, pusticulate, colliculate and smooth) were found. The number of testa cells per 100 µm2 and also its range, from 8–12 to 57–63, are given.

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STUDIES ON INFLAMMATION

Journal of Experimental Medicine ◽

10.1084/jem.53.5.647 ◽

1931 ◽

Vol 53 (5) ◽

pp. 647-660 ◽

Cited By ~ 21

Author(s):

Valy Menkin

Keyword(s):

Lymph Nodes ◽

Peritoneal Cavity ◽

Normal Tissue ◽

Capillary Permeability ◽

Blood Stream ◽

Regional Lymph Nodes ◽

India Ink ◽

Fibrin Network ◽

Free Particles

India ink or graphite partides injected into an area of inflammation fail to disseminate to the tributary lymph nodes. When injected into a normal peritoneal cavity they rapidly appear in the retrosternal lymph nodes. When injected into an inflamed peritoneal cavity they are fixed in situ and fail to reach the regional lymph nodes. Graphite particles injected in the circulating blood stream enter an inflamed area both as free particles owing to increased capillary permeability and also as phagocyted material within leucocytes. Bacteria (B. prodigiosus) injected into inflamed tissue are fixed at the site of inflammation and fail to disseminate to the regional lymph nodes as readily as when injected into normal tissue. Bacteria (B. prodigiosus) injected at the periphery of an inflamed area do not readily penetrate into the site of inflammation. The experiments furnish evidence, in addition to that already provided, that fixation of foreign substances by the inflammatory reaction is primarily due to mechanical obstruction caused by a fibrin network and by thrombosed lymphatics at the site of inflammation. Bacteria (B. prodigiosus and B. pyocyaneus) injected intravenously rapidly enter an inflamed area. It is suggested that localization of bacteria in a locus minoris resistentiae may be explained as the result of increased capillary permeability with subsequent accumulation and fixation of bacteria from the blood stream at the point of injury.

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Langerin (CD207) Staining in Normal Pediatric Tissues, Reactive LymphNodes, and Childhood Histiocytic Disorders

Pediatric and Developmental Pathology ◽

10.1007/s10024-004-3027-z ◽

2004 ◽

Vol 7 (6) ◽

pp. 607-614 ◽

Cited By ~ 88

Author(s):

Kudakwashe Chikwava ◽

Ronald Jaffe

Keyword(s):

Lymph Nodes ◽

Staining Pattern ◽

Granular Cell ◽

Fixed Tissue ◽

Cytoplasmic Staining ◽

Immunohistochemical Markers ◽

Rare Cells ◽

Childhood Histiocytic Disorders ◽

Cytoplasmic Staining Pattern ◽

Skin Samples

Langerin is a recently identified lectin for which antibodies can be used as immunohistochemical markers of Langerhans cells (LCs). We describe the distribution of staining in autopsy pediatric tissues, dermatopathic and other reactive lymph nodes, and childhood histiocytic lesions using the 12D6 antibody (Novocastra). We also correlate CD1a (antibody O1O) staining to these factors. Langerin on epidermal LCs has a coarsely granular cell membrane and a cytoplasmic staining pattern that is always associated with CD1a expression. All 6 skin samples had Langerin+/CD1a+ LCs within the epidermis. Six of 8 thymuses showed single scattered dendritic-shaped cells in the medulla and rare cells within Hassall corpuscles that coexpressed Langerin and CD1a. Cortical thymocytes were CD1a+/Langerin-. Four of 8 livers examined showed a sinusoidal lining pattern of Langerin+/CD1a-. All 15 autopsy lymph nodes showed a similarly strong Langerin+/CD1a- sinus pattern of staining on fixed tissue elements, mostly in medullary sinuses. All 12 dermatopathic lymph nodes showed accumulation of Langerin+/CD1a+ cells in the pale paracortical nodules. All 24 instances of LC histiocytosis (LCH) were Langerin+/CD1a+. All 12 non-LCH histiocytic disorders are negative for Langerin in the histiocytes of interest. We conclude that Langerin is coexpressed with CD1a on LCs and LCH. Lymph node sinuses and hepatic sinusoids show Langerin+/CD1a-cells, indicating that, when used alone to confirm LCH infiltration, the 12D6 antibody should be used with caution. At other sites, its diagnostic accuracy is similar to that of CD1a.

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