The Cholinergic and Purinergic Components of Detrusor Contractility in a Whole Rabbit Bladder Model

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

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Inhibition of detrusor contractility by a flavonoid fraction of Bryophyllum pinnatum - a new option to treat the overactive bladder syndrome

Planta Medica ◽

10.1055/s-0033-1351963 ◽

2013 ◽

Vol 79 (13) ◽

Cited By ~ 4

Author(s):

K Fürer ◽

D Eberli ◽

C Betschart ◽

R Brenneisen ◽

M Hamburger ◽

...

Keyword(s):

Overactive Bladder ◽

Overactive Bladder Syndrome ◽

Detrusor Contractility ◽

Flavonoid Fraction ◽

Bryophyllum Pinnatum

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Faculty Opinions recommendation of Treatment of post-prostatectomy incontinence with male slings in patients with impaired detrusor contractility on urodynamics and/or who perform Valsalva voiding.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.13296971.14657087 ◽

2011 ◽

Author(s):

Alexis Te ◽

Doreen E Chung

Keyword(s):

Detrusor Contractility

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Predictive Factors in Sacral Neuromodulation: A Systematic Review

Urologia Internationalis ◽

10.1159/000513937 ◽

2021 ◽

pp. 1-21

Author(s):

Ranjana Jairam ◽

Jamie Drossaerts ◽

Tom Marcelissen ◽

Gommert van Koeveringe ◽

Desiree Vrijens ◽

...

Keyword(s):

Systematic Review ◽

Predictive Factors ◽

Sacral Neuromodulation ◽

Level Of Evidence ◽

Specific Patient ◽

Detrusor Contractility ◽

Affective Symptoms ◽

Lower Success Rate ◽

Patient Groups ◽

Based Medicine

Introduction: Sacral neuromodulation (SNM) is an effective treatment in patients with overactive bladder syndrome or nonobstructive urinary retention when conservative treatment fails. Several factors that could impact outcome with SNM have been studied. This systematic review investigated these predictive factors and their relevance for clinical practice. Methods: Systematic review according to the PRISMA guidelines was conducted. This review is registered in the PROSPERO register (CRD42015016256). Results: Seventy-eight studies (of which 11 abstracts) were included. Females, younger patients, and a tined lead procedure tend to be predictive in successful SNM outcome. Factors that did not influence SNM outcome were prior back surgery, surgery for stress urinary incontinence, affective symptoms, and duration of complaints. Reduced detrusor contractility is associated with a lower success rate. The level of evidence of most studies (graded according to the Centre for Evidence-Based Medicine) was 3b. Conclusion: Even though this systematic review investigated predictive factors (gender, age, type of procedure, type of lead, and detrusor contractility), no general consensus on predictive factors could be made. Most studies are small, retrospective, and involve a heterogeneous population. Therefore, prospective research in larger specific patient groups remains necessary to find predictors of SNM outcome.

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Tetrodotoxin-resistant release of ATP from superfused rabbit detrusor muscle during electrical field stimulation in the presence of luciferin–luciferase

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y84-026 ◽

1984 ◽

Vol 62 (1) ◽

pp. 153-156 ◽

Cited By ~ 15

Author(s):

Archana Chaudhry ◽

John W. Downie ◽

Thomas D. White

Keyword(s):

Action Potentials ◽

Electrical Field Stimulation ◽

Detrusor Muscle ◽

Electrical Field ◽

Stimulation Parameters ◽

Rabbit Bladder ◽

Firefly Luciferin ◽

Rabbit Urinary Bladder ◽

Stimulation Of

The present study was carried out to assess the possible role of ATP in the noncholinergic, nonadrenergic transmission in the rabbit urinary bladder. When rabbit detrusor muscle strips were superfused with medium containing firefly luciferin–luciferase and stimulated transmurally at low stimulation parameters, tetrodotoxin-sensitive contractions were obtained but no release of ATP could be detected. However, at somewhat higher stimulation parameters, release of ATP was observed. This release of ATP was not diminished by tetrodotoxin indicating that ATP was not likely released as a result of propagated action potentials in nerves. Because contractions persisted in the presence of tetrodotoxin, it is possible that the ATP might have been released as a result of direct electrical stimulation of the muscle. These results do not support the idea that ATP is released as a neurotransmitter in the rabbit bladder.

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