scholarly journals Focus on the glycerophosphocholine pathway in choline phospholipid metabolism of cancer

2019 ◽  
Vol 32 (10) ◽  
Author(s):  
Kanchan Sonkar ◽  
Vinay Ayyappan ◽  
Caitlin M. Tressler ◽  
Oluwatobi Adelaja ◽  
Ruoqing Cai ◽  
...  
Keyword(s):  
Phospholipid Metabolism ◽  
Choline Phospholipid

Therapeutic targets and biomarkers identified in cancer choline phospholipid metabolism

2006 ◽  
Vol 7 (7) ◽  
pp. 1109-1123 ◽  
Author(s):  
Kristine Glunde ◽  
Natalie J Serkova
Keyword(s):  
Therapeutic Targets ◽  
Phospholipid Metabolism ◽  
Choline Phospholipid

Abstract 5162: COX-2 downregulation protects SUM-149 cells following targeting of choline phospholipid metabolism

2012 ◽  
Author(s):  
Noriko Mori ◽  
Ioannis Stasinopoulos ◽  
Flonne Wildes ◽  
Zaver M. Bhujwalla
Keyword(s):  
Phospholipid Metabolism ◽  
Choline Phospholipid ◽  
Cox 2

Alterations of Choline Phospholipid Metabolism in Ovarian Tumor Progression

2005 ◽  
Vol 65 (20) ◽  
pp. 9369-9376 ◽  
Author(s):  
Egidio Iorio ◽  
Delia Mezzanzanica ◽  
Paola Alberti ◽  
Francesca Spadaro ◽  
Carlo Ramoni ◽  
...  
Keyword(s):  
Tumor Progression ◽  
Ovarian Tumor ◽  
Phospholipid Metabolism ◽  
Choline Phospholipid

scholarly journals The Impact of Freezing Temperature and Pretreatment Protocol on Solid Phase HR-MAS-MRS Metabolomes of Colorectal Cancer Tissue

2021 ◽  
Author(s):  
Hui Liu ◽  
Yu Wang ◽  
Yue-qiang Han ◽  
Guang-yu Yang ◽  
Lu Wang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Liquid Nitrogen ◽  
Solid Phase ◽  
Phospholipid Metabolism ◽  
The Other ◽  
Cancer Tissue ◽  
Nitrogen Storage ◽  
Tissue Samples ◽  
Colorectal Cancer Tissue ◽  
Choline Phospholipid

Abstract Background: To explore the best pretreatment method of colorectal cancer tissue samples for metabolomics research based on solid-phase nuclear magnetic resonance. Method: Taking mucosal tissues of colorectal cancer and divide it into 5 groups of 0.2cm*0.2cm*0.2cm. Pretreatment was performed as follows: I. Liquid nitrogen storage; II. Transfer to the -80℃ refrigerator after storing in liquid nitrogen for 10 minutes; III. Transfer to the -80℃ refrigerator after storing in liquid nitrogen for 20 minutes; IV. Transfer to the -80℃ refrigerator after storing in liquid nitrogen for 30 minutes; V. -80℃ refrigerator storage. The interval between tumor sample separation to pretreatment is less than 30 minutes. The tissue sample testing process is carried out on Bruker AVII-600 Spectrometer equipped with a high-resolution probe having a 1H/13C magical angle rotation. The tissue samples were put into the NMR which run at a speed of 5000Hz for 10 minutes. NMR signals were collected and analyzed by Fourier transform, partial least squares discrimination analysis (PLS-DA). Corresponding metabolites and metabolic pathways were found in Human Metabolome Database (HMDB) according to the ppms with variable importance of projection (VIP) ＞1. Results: The content of pelargonic acid, stearic acid, D-Ribose, heptadecanoic acid, pyruvic acid, succinate, sarcosine, glycine, creatine, and L-lactate in liquid nitrogen storage group were significantly lower than the other groups (P<0.05), the content of glycerophosphocholine in liquid nitrogen storage group was lower than the other groups (P=0.055). Pyruvic, succinate and L-lactate are participating in glucose metabolism. Glycerophosphocholine, sarcosine, glycine and creatine are participating in choline phospholipid metabolism. This indicated that the glucose and choline phospholipid metabolism levels of the liquid nitrogen group were significantly lower than those of the other 4 groups. Conclusion: Liquid nitrogen storage can slow down the glucose and choline phospholipid metabolism process of colorectal cancer tissue samples in vitro; liquid nitrogen can preserve tissue sample’s metabolic state in the body. It is therefore the better way to store tissue sample than the other methods. clinical trial registry website: http://www.chictr.org.cn/index.aspx. Trial number: ChiCTR1900024640

Abstract 1902: Characterization of choline phospholipid metabolism in pancreatic cancer cells.

2013 ◽  
Author(s):  
Tariq Shah ◽  
Flonne Wildes ◽  
Yelena Mironchik ◽  
Marie-France Penet ◽  
Anirban Maitra ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cancer Cells ◽  
Phospholipid Metabolism ◽  
Pancreatic Cancer Cells ◽  
Choline Phospholipid

scholarly journals Choline Phospholipid Metabolites of Human Vascular Endothelial Cells Altered by Cyclooxygenase Inhibition, Growth Factor Depletion, and Paracrine Factors Secreted by Cancer Cells

2003 ◽  
Vol 2 (2) ◽  
pp. 153535002003031
Author(s):  
Noriko Mori ◽  
Kshama Natarajan ◽  
V. P. Chacko ◽  
Dmitri Artemov ◽  
Zaver M. Bhujwalla
Keyword(s):  
Endothelial Cells ◽  
Growth Factors ◽  
Growth Factor ◽  
Cancer Cells ◽  
Umbilical Vein ◽  
Phospholipid Metabolism ◽  
Paracrine Factors ◽  
Inflammatory Agent ◽  
Choline Phospholipid ◽  
Anti Inflammatory

Magnetic resonance studies have previously shown that solid tumors and cancer cells in culture typically exhibit high phosphocholine and total choline. Treatment of cancer cells with the anti-inflammatory agent, indomethacin (INDO), reverted the phenotype of choline phospholipid metabolites in cancer cells towards a less malignant phenotype. Since endothelial cells form a key component of tumor vasculature, in this study, we used MR spectroscopy to characterize the phenotype of choline phospholipid metabolites in human umbilical vein endothelial cells (HUVECs). We determined the effect of growth factors, the anti-inflammatory agent INDO, and conditioned media obtained from a malignant cell line, on choline phospholipid metabolites. Growth factor depletion or treatment with INDO induced similar changes in the choline phospholipid metabolites of HUVECs. Treatment with conditioned medium obtained from MDA-MB-231 cancer cells induced changes similar to the presence of growth factor supplements. These results suggest that cancer cells secrete growth factors and/or other molecules that influence the choline phospholipid metabolism of HUVECs. The ability of INDO to alter choline phospholipid metabolism in the presence of growth factor supplements suggests that the inflammatory response pathways of HUVECs may play a role in cancer cell-HUVEC interaction and in the response of HUVECs to growth factors.

Choline Phospholipid Metabolism in Cancer:  Consequences for Molecular Pharmaceutical Interventions

2006 ◽  
Vol 3 (5) ◽  
pp. 496-506 ◽  
Author(s):  
Kristine Glunde ◽  
Ellen Ackerstaff ◽  
Noriko Mori ◽  
Michael A. Jacobs ◽  
Zaver M. Bhujwalla
Keyword(s):  
Phospholipid Metabolism ◽  
Choline Phospholipid ◽  
Pharmaceutical Interventions

scholarly journals Mechanisms of Indomethacin-Induced Alterations in the Choline Phospholipid Metabolism of Breast Cancer Cells

Neoplasia ◽  
2006 ◽  
Vol 8 (9) ◽  
pp. 758-771 ◽  
Author(s):  
Kristine Glunde ◽  
Chunfa Jie ◽  
Zaver M. Bhujwalla
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Phospholipid Metabolism ◽  
Choline Phospholipid

Pathophysiological analysis of primary biliary cirrhosis focusing on choline/phospholipid metabolism

2014 ◽  
Vol 35 (3) ◽  
pp. 1095-1102 ◽  
Author(s):  
Motoyuki Kohjima ◽  
Munechika Enjoji ◽  
Ryoko Yada ◽  
Tsuyoshi Yoshimoto ◽  
Tsukasa Nakamura ◽  
...  
Keyword(s):  
Primary Biliary Cirrhosis ◽  
Phospholipid Metabolism ◽  
Biliary Cirrhosis ◽  
Choline Phospholipid
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