Correlation between lactate dehydrogenase/pyruvate dehydrogenase activities ratio and tissue pH in the perfused mouse heart: A potential noninvasive indicator of cardiac pH provided by hyperpolarized magnetic resonance

2020 ◽  
Vol 34 (2) ◽  
Author(s):  
David Shaul ◽  
Assad Azar ◽  
Gal Sapir ◽  
Sivaranjan Uppala ◽  
Atara Nardi‐Schreiber ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Magnetic Resonance ◽  
Pyruvate Dehydrogenase ◽  
Mouse Heart ◽  
Tissue Ph

scholarly journals Metabolic compartmentalization in the human cortex and hippocampus: evidence for a cell- and region-specific localization of lactate dehydrogenase 5 and pyruvate dehydrogenase

2007 ◽  
Vol 8 (1) ◽  
Author(s):  
Jocelyn D Laughton ◽  
Philippe Bittar ◽  
Yves Charnay ◽  
Luc Pellerin ◽  
Enikö Kovari ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Pyruvate Dehydrogenase ◽  
Human Cortex ◽  
Specific Localization ◽  
A Cell

Exercise training increases electron and substrate shuttling proteins in muscle of overweight men and women with the metabolic syndrome

2005 ◽  
Vol 98 (1) ◽  
pp. 168-179 ◽  
Author(s):  
Dustin S. Hittel ◽  
William E. Kraus ◽  
Chuck J. Tanner ◽  
Joseph A. Houmard ◽  
Eric P. Hoffman
Keyword(s):  
Metabolic Syndrome ◽  
Lactate Dehydrogenase ◽  
Pyruvate Dehydrogenase ◽  
Oxidative Metabolism ◽  
Aspartate Aminotransferase ◽  
Aerobic Training ◽  
Exercise Intervention ◽  
Complex Iii ◽  
Men And Women ◽  
The Metabolic Syndrome

Aerobic conditioned muscle shows increased oxidative metabolism or glucose relative to untrained muscle at a given absolute exercise intensity. The studies of a targeted risk reduction intervention through defined exercise (STRRIDE) study is an aerobic exercise intervention in men and women with features of metabolic syndrome (Kraus WE, Torgan CE, Duscha BD, Norris J, Brown SA, Cobb FR, Bales CW, Annex BH, Samsa GP, Houmard JA, and Slentz CA, Med Sci Sports Exerc 33: 1774–1784, 2001), with four muscle biopsies taken during training and detraining time points. Here, we expanded a previous study (Hittel DS, Kraus WE, and Hoffman EP, J Physiol 548: 401–410, 2003) and used mRNA profiling to investigate gene transcripts associated with energy and substrate metabolism in STRRIDE participants. We found coordinate regulation of key metabolic enzymes with aerobic training in metabolic syndrome (aspartate aminotransferase 1, lactate dehydrogenase B, and pyruvate dehydrogenase-α1). All were also quickly downregulated by detraining, although the induction was not an acute response to activity. Protein and enzymatic assays were used to validate mRNA induction with aerobic training and loss with detraining (96 h to 2 wk) in 10 male and 10 female STRRIDE subjects. We propose that training coordinately increases the levels of aspartate aminotransferase 1, lactate dehydrogenase B, and pyruvate dehydrogenase-α1 subunit, increasing glucose metabolism in muscle by liberating pyruvate for oxidative metabolism and, therefore, limiting lactate efflux. Serial measurement of fasting plasma lactate from 62 subjects from the same exercise group demonstrated a significant decrease of circulating lactate with training. We also found evidence for sex-specific molecular remodeling of muscle with ubiquinol-cytochrome c reductase core protein II, a component of mitochondrial respiratory complex III, which showed an increase after training that was specific to women. These biochemical adaptations complement existing molecular models for improved glucose tolerance with exercise intervention in prediabetic individuals.

scholarly journals Increasing Pyruvate Dehydrogenase Flux as a Treatment for Diabetic Cardiomyopathy: A Combined13C Hyperpolarized Magnetic Resonance and Echocardiography Study

Diabetes ◽  
2015 ◽  
Vol 64 (8) ◽  
pp. 2735-2743 ◽  
Author(s):  
Lydia M. Le Page ◽  
Oliver J. Rider ◽  
Andrew J. Lewis ◽  
Vicky Ball ◽  
Kieran Clarke ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Diabetic Cardiomyopathy ◽  
Pyruvate Dehydrogenase

An improved spectrophotometric assay of pyruvate dehydrogenase in lactate dehydrogenase contaminated mitochondrial preparations from human skeletal muscle

1995 ◽  
Vol 240 (2) ◽  
pp. 129-136 ◽  
Author(s):  
Dominique Chretien ◽  
Marc Pourrier ◽  
Thomas Bourgeron ◽  
Maï Séné ◽  
Agnès Rötig ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Lactate Dehydrogenase ◽  
Pyruvate Dehydrogenase ◽  
Human Skeletal Muscle ◽  
Spectrophotometric Assay

Open-chest31P magnetic resonance spectroscopy of mouse heart at 4.7 Tesla

2006 ◽  
Vol 24 (6) ◽  
pp. 1269-1276 ◽  
Author(s):  
Joseph Lee ◽  
Qingsong Hu ◽  
Yasuhiro Nakamura ◽  
Xiaohong Wang ◽  
Xiaoliang Zhang ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Resonance Spectroscopy ◽  
Mouse Heart

scholarly journals Proportion of active dephosphorylated pyruvate dehydrogenase complex in heart and isolated heart mitochondria is decreased in obese hyperinsulinaemic mice

1984 ◽  
Vol 217 (1) ◽  
pp. 117-121 ◽  
Author(s):  
A L Kerbey ◽  
I D Caterson ◽  
P F Williams ◽  
J R Turtle
Keyword(s):  
Insulin Resistance ◽  
Enzyme Activity ◽  
Pyruvate Dehydrogenase ◽  
Glucose Oxidation ◽  
Isolated Heart ◽  
Pyruvate Dehydrogenase Complex ◽  
Heart Mitochondria ◽  
Mouse Heart ◽  
Inhibitory Effects ◽  
Dehydrogenase Complex

The proportion of active, dephosphorylated, pyruvate dehydrogenase complex was decreased in the mouse heart by obesity (by 56%), and this decrease in enzyme activity persisted during preparation and extraction of heart mitochondria. Phosphorylation and inactivation of pyruvate dehydrogenase may be a major factor in mediating the inhibitory effects of obesity on glucose oxidation in muscle, and this may represent an important mechanism in the development and/or expression of cellular insulin-resistance.

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