scholarly journals Coupling 3D and 1D Skeletal Muscle Models

PAMM ◽  
2012 ◽  
Vol 12 (1) ◽  
pp. 111-112
Author(s):  
Michael Sprenger ◽  
Syn Schmitt ◽  
Oliver Röhrle
Keyword(s):  
Skeletal Muscle ◽  
Muscle Models

Angiogenesis in the Heart and Skeletal Muscle — Models for Capillary Growth

Angiogenesis ◽  
1998 ◽  
pp. 19-33 ◽  
Author(s):  
O. Hudlicka ◽  
S. Egginton ◽  
M. D. Brown
Keyword(s):  
Skeletal Muscle ◽  
Capillary Growth ◽  
Muscle Models

scholarly journals Bioprinting of 3D in vitro skeletal muscle models: A review

2020 ◽  
Vol 193 ◽  
pp. 108794 ◽  
Author(s):  
Pei Zhuang ◽  
Jia An ◽  
Chee Kai Chua ◽  
Lay Poh Tan
Keyword(s):  
Skeletal Muscle ◽  
Muscle Models

Differences Between Principal Strain Directions and Muscle Fiber Directions in the Contracting Biceps Bracii Measured With CPC-MRI

2007 ◽  
Author(s):  
Hehe Zhou ◽  
John E. Novotny
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fiber ◽  
Biceps Brachii ◽  
Principal Strain ◽  
Strain Fields ◽  
Ultrasound Study ◽  
Lagrangian Strain ◽  
Novel Method ◽  
Muscle Models

Skeletal muscle exhibit non-uniform internal architectures and our research goals are to relate these to their internal mechanics during contraction to provide a better understanding of muscle function, verify muscle models and permit more sophisticated interpretation of the functional effects of injury. Our previous studies have developed a novel method to calculate 2D Lagrangian strain field of skeletal muscle from the CPC-MRI [1]. The objective of this study is to apply CPC-MRI to derive Lagrangian strain fields for the normal biceps brachii and to determine its minimum principal strain directions in vivo during contraction. We will then compare them to the muscle fiber directions or pennation angles measured by a parallel ultrasound study. If we assume the muscle force is mainly transmitted along fiber directions, we would expect that the minimum principal strain directions are the same as fiber directions. Comparisons between different regions of the muscle and flexion angles will also be made.

scholarly journals Model order reduction of dynamic skeletal muscle models

PAMM ◽  
2016 ◽  
Vol 16 (1) ◽  
pp. 851-852
Author(s):  
Mylena Mordhorst ◽  
Daniel Wirtz ◽  
Oliver Röhrle
Keyword(s):  
Skeletal Muscle ◽  
Model Order Reduction ◽  
Order Reduction ◽  
Model Order ◽  
Muscle Models

Preconditioning improves function and recovery of single muscle fibers during severe hypoxia and reoxygenation

2001 ◽  
Vol 281 (1) ◽  
pp. C142-C146 ◽  
Author(s):  
Suzanne Kohin ◽  
Creed M. Stary ◽  
Richard A. Howlett ◽  
Michael C. Hogan
Keyword(s):  
Skeletal Muscle ◽  
Ischemic Preconditioning ◽  
Muscle Fibers ◽  
Hypoxic Preconditioning ◽  
Cellular Damage ◽  
Severe Hypoxia ◽  
Single Muscle ◽  
Cell Recovery ◽  
Skeletal Muscle Fibers ◽  
Muscle Models

Reperfusion following prolonged ischemia induces cellular damage in whole skeletal muscle models. Ischemic preconditioning attenuates the deleterious effects. We tested whether individual skeletal muscle fibers would be similarly affected by severe hypoxia and reoxygenation (H/R) in the absence of extracellular factors and whether cellular damage could be alleviated by hypoxic preconditioning. Force and free cytosolic Ca2+([Ca2+]c) were monitored in Xenopus single muscle fibers ( n = 24) contracting tetanically at 0.2 Hz during 5 min of severe hypoxia and 5 min of reoxygenation. Twelve cells were preconditioned by a shorter bout of H/R 1 h before the experimental trial. In preconditioned cells, force relative to initial maximal values (P/Po) and relative peak [Ca2+]c fell ( P< 0.05) during 5 min of hypoxia and recovered during reoxygenation. In contrast, P/Po and relative peak [Ca2+]c fell more during hypoxia ( P < 0.05) and recovered less during reoxygenation ( P < 0.05) in control cells. The ratio of force to [Ca2+]c was significantly higher in the preconditioned cells during severe hypoxia, suggesting that changes in [Ca2+]c were not solely responsible for the loss in force. We conclude that 1) isolated skeletal muscle fibers contracting in the absence of extracellular factors are susceptible to H/R injury associated with changes in Ca2+handling; and 2) hypoxic preconditioning improves contractility, Ca2+ handling, and cell recovery during subsequent hypoxic insult.

Engineering Murine Adipocytes and Skeletal Muscle Cells in Meat-like Constructs Using Self-Assembled Layer-by-Layer Biofabrication: A Platform for Development of Cultivated Meat

2021 ◽  
pp. 1-9
Author(s):  
Alireza Shahin-Shamsabadi ◽  
P. Ravi Selvaganapathy
Keyword(s):  
Skeletal Muscle ◽  
Self Assembly ◽  
Arable Land ◽  
Animal Husbandry ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Layer By Layer ◽  
Cell Sheets ◽  
Self Assembled Layer ◽  
Muscle Models

Global meat consumption has been growing on a per capita basis over the past 20 years resulting in ever-increasing devotion of resources in the form of arable land and potable water to animal husbandry which is unsustainable and inefficient. One approach to meet this insatiable demand is to use biofabrication methods used in tissue engineering in order to make skeletal muscle tissue-like constructs known as cultivated meat to be used as a food source. Here, we demonstrate the use of a scaffold-free biofabrication method that forms cell sheets composed of murine adipocytes and skeletal muscle cells and assembles these sheets in parallel to create a 3D meat-like construct without the use of any exogenous materials. This layer-by-layer self-assembly and stacking process is fast (4 days of culture to form sheets and few hours for assembly) and scalable (stable sheets with diameters &#x3e;3 cm are formed). Tissues formed with only muscle cells were equivalent to lean meat with comparable protein and fat contents (lean beef had 1.5 and 0.9 times protein and fat, respectively, as our constructs) and incorporating adipocyte cells in different ratios to myoblasts and/or treatment with different media cocktails resulted in a 5% (low fat meat) to 35% (high fat meat) increase in the fat content. Not only such constructs can be used as cultivated meat, they can also be used as skeletal muscle models.

scholarly journals Tissue-Engineered Skeletal Muscle Models to Study Muscle Function, Plasticity, and Disease

2021 ◽  
Vol 12 ◽  
Author(s):  
Alastair Khodabukus
Keyword(s):  
Skeletal Muscle ◽  
Animal Models ◽  
Muscle Fiber ◽  
Muscle Function ◽  
Fiber Type ◽  
Small Animal ◽  
Muscle Fiber Type ◽  
Small Animal Models ◽  
Muscle Models

Skeletal muscle possesses remarkable plasticity that permits functional adaptations to a wide range of signals such as motor input, exercise, and disease. Small animal models have been pivotal in elucidating the molecular mechanisms regulating skeletal muscle adaptation and plasticity. However, these small animal models fail to accurately model human muscle disease resulting in poor clinical success of therapies. Here, we review the potential of in vitro three-dimensional tissue-engineered skeletal muscle models to study muscle function, plasticity, and disease. First, we discuss the generation and function of in vitro skeletal muscle models. We then discuss the genetic, neural, and hormonal factors regulating skeletal muscle fiber-type in vivo and the ability of current in vitro models to study muscle fiber-type regulation. We also evaluate the potential of these systems to be utilized in a patient-specific manner to accurately model and gain novel insights into diseases such as Duchenne muscular dystrophy (DMD) and volumetric muscle loss. We conclude with a discussion on future developments required for tissue-engineered skeletal muscle models to become more mature, biomimetic, and widely utilized for studying muscle physiology, disease, and clinical use.

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