CAGW and TAT‐NLS peptides functionalized multitargeting gene delivery system with high transfection efficiency

The key strategy for the advancement of gene therapy is the development of an efficient targeted gene delivery system into cells. The targeted gene delivery system is especially important in non-viral gene transfer which shows the relatively low transfection efficiency. It also opens the possibility of selective delivery of therapeutic plasmids to specific tissues. Chitosan has been considered to be a good candidate for gene delivery system, since it is already known as a biocompatible, biodegradable, and low toxic material with high cationic potential. However, low specificity and low transfection efficiency of chitosan need to be overcome prior to clinical trial. In this study, we focused on the chemical modification of chitosan for enhancement of cell specificity and transfection efficiency. Also, the potential of clinical application was investigated.

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A versatile endosome acidity-induced sheddable gene delivery system: increased tumor targeting and enhanced transfection efficiency

International Journal of Nanomedicine ◽

10.2147/ijn.s215250 ◽

2019 ◽

Vol Volume 14 ◽

pp. 6519-6538 ◽

Cited By ~ 3

Author(s):

Ming Zhao ◽

Ji Li ◽

Hongrui Ji ◽

Dawei Chen ◽

Haiyang Hu

Keyword(s):

Gene Delivery ◽

Delivery System ◽

Tumor Targeting ◽

Transfection Efficiency ◽

Gene Delivery System

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Zinc ion coordination significantly improved the transfection efficiency of low molecular weight polyethylenimine

Biomaterials Science ◽

10.1039/c9bm00039a ◽

2019 ◽

Vol 7 (4) ◽

pp. 1716-1728 ◽

Cited By ~ 4

Author(s):

Huapan Fang ◽

Lin Lin ◽

Jie Chen ◽

Jiayan Wu ◽

Huayu Tian ◽

...

Keyword(s):

Molecular Weight ◽

Gene Delivery ◽

Delivery System ◽

Transfection Efficiency ◽

Zinc Ion ◽

Low Molecular Weight ◽

Gene Delivery System ◽

System P

A zinc ion coordination-contained polycationic gene delivery system.

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A Poly(β-Amino Ester) of Spermine and Poly(ethylene Glycol) Diacrylate as a Gene Carrier

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.342-343.425 ◽

2007 ◽

Vol 342-343 ◽

pp. 425-428 ◽

Cited By ~ 8

Author(s):

Dhananjay Jere ◽

Tae Hee Kim ◽

Rohidas B. Arote ◽

Hu Lin Jiang ◽

Myung Haing Cho ◽

...

Keyword(s):

Gene Delivery ◽

Ethylene Glycol ◽

Delivery System ◽

Transfection Efficiency ◽

Water Soluble ◽

Gene Carrier ◽

Gene Delivery System ◽

Poly Ethylene Glycol ◽

Wide Range ◽

Poly Ethylene

Vectors are vital aspect of gene delivery system which decides the success of gene therapy. Efficient transfection with minimum or no toxicity, are two principal aims of any gene delivery system. In this our study, we rationally developed biodegradable water soluble poly(ßamino ester) (PAE) based on spermine (SPR) and poly (ethylene glycol) (PEG), by Michael-type addition reaction and further studied for its potential as a gene carrier. Confirmation of synthesized PAE was done by proton NMR spectroscopy. In gel retardation assay, the PAEs have shown good DNA binding ability over wide range of polyplexes. The addition of PEG over SPR resulted in a novel PAE with higher degree of safety and transfection efficiency as compared with polyethylenimine 25K (PEI) when studied in 293T human kidney carcinoma cells.

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PEI-g-chitosan, a Novel Gene Delivery System with Transfection Efficiency Comparable to Polyethylenimine in Vitro and after Liver Administration in Vivo

Bioconjugate Chemistry ◽

10.1021/bc0501597 ◽

2006 ◽

Vol 17 (1) ◽

pp. 152-158 ◽

Cited By ~ 203

Author(s):

Kokhou Wong ◽

Guobin Sun ◽

Zhang ◽

Hui Dai ◽

Ye Liu ◽

...

Keyword(s):

Gene Delivery ◽

Delivery System ◽

Transfection Efficiency ◽

Gene Delivery System ◽

Novel Gene

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Receptor-Mediated Gene Delivery Using Chitosan Derivatives In Vitro and In Vivo

Materials Science Forum ◽

10.4028/www.scientific.net/msf.539-543.641 ◽

2007 ◽

Vol 539-543 ◽

pp. 641-646 ◽

Cited By ~ 1

Author(s):

Tae Hee Kim ◽

Jin Hua ◽

Hyun Woo Kim ◽

Myung Haing Cho ◽

Jae Woon Nah ◽

...

Keyword(s):

Gene Delivery ◽

Delivery System ◽

Transfection Efficiency ◽

Viral Gene ◽

Gene Delivery System ◽

Targeted Gene Delivery ◽

Cell Specificity ◽

Selective Delivery

The development of an efficient targeted gene delivery system into cells is an important strategy for the advancement of gene therapy. The targeted gene delivery system is especially important in non-viral gene transfer which shows the relative low transfection efficiency. And it also opens the possibility of selective delivery of therapeutic plasmids to specific tissues. Chitosan has been considered to be a good candidate for gene delivery system, since it is already known as a biocompatible, biodegradable, and low toxic material with high cationic potential. However, low specificity and low transfection efficiency of chitosan need to be overcome prior to clinical trial. In this study, we focused on the chemical modification of chitosan for enhancement of cell specificity and transfection efficiency.

Download Full-text