Effect of electric stimulus on human adipose‐derived mesenchymal stem cells cultured in 3D ‐printed scaffolds

2020 ◽  
Author(s):  
Tuba Bedir ◽  
Songul Ulag ◽  
Kivanc Aydogan ◽  
Ali Sahin ◽  
Betul Karademir Yilmaz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electric Stimulus ◽  
3D Printed ◽  
Cells Cultured

Morphology and Differentiation of Human Mesenchymal Stem Cells Cultured on a Nano-Scale Structured Substrate

2020 ◽  
Vol 140 (4) ◽  
pp. 432-436
Author(s):  
Masashi Yamazaki ◽  
Hiroki Kojima ◽  
Hiromi Miyoshi
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Nano Scale ◽  
Cells Cultured

Menaquinone‐4 enhances osteogenic potential of human amniotic fluid mesenchymal stem cells cultured in 2D and 3D dynamic culture systems

2017 ◽  
Vol 12 (2) ◽  
pp. 447-459 ◽  
Author(s):  
Domitilla Mandatori ◽  
Letizia Penolazzi ◽  
Caterina Pipino ◽  
Pamela Di Tomo ◽  
Sara Di Silvestre ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Amniotic Fluid ◽  
Osteogenic Potential ◽  
Human Amniotic Fluid ◽  
Culture Systems ◽  
Dynamic Culture ◽  
2D And 3D ◽  
Cells Cultured

Effect of electrical stimulation on chondrogenic differentiation of mesenchymal stem cells cultured in hyaluronic acid – Gelatin injectable hydrogels

2020 ◽  
Vol 134 ◽  
pp. 107536 ◽  
Author(s):  
Juan Jairo Vaca-González ◽  
Sandra Clara-Trujillo ◽  
María Guillot-Ferriols ◽  
Joaquín Ródenas-Rochina ◽  
María J. Sanchis ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electrical Stimulation ◽  
Hyaluronic Acid ◽  
Chondrogenic Differentiation ◽  
Injectable Hydrogels ◽  
Cells Cultured

Mesenchymal stem cells cultivated on scaffolds formed by 3D printed PCL matrices, coated with PLGA electrospun nanofibers for use in tissue engineering

2017 ◽  
Vol 3 (4) ◽  
pp. 045005 ◽  
Author(s):  
Natasha Maurmann ◽  
Daniela P Pereira ◽  
Daniela Burguez ◽  
Frederico D A de S Pereira ◽  
Paulo Inforçatti Neto ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electrospun Nanofibers ◽  
3D Printed

In vitro Mineralization by Mesenchymal Stem Cells Cultured on Titanium Scaffolds

2007 ◽  
Vol 141 (5) ◽  
pp. 729-736 ◽  
Author(s):  
Masahiko Maeda ◽  
Motohiro Hirose ◽  
Hajime Ohgushi ◽  
Tadaaki Kirita
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Mineralization ◽  
Cells Cultured

scholarly journals Evaluation of synovium-derived mesenchymal stem cells and 3D printed nanocomposite scaffolds for tissue engineering

2015 ◽  
Vol 16 (4) ◽  
pp. 045001 ◽  
Author(s):  
Jian-Feng Pan ◽  
Shuo Li ◽  
Chang-An Guo ◽  
Du-Liang Xu ◽  
Feng Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
3D Printed ◽  
Nanocomposite Scaffolds

scholarly journals Effects of Hypoxia and Chitosan on Equine Umbilical Cord-Derived Mesenchymal Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
D. J. Griffon ◽  
J. Cho ◽  
J. R. Wagner ◽  
C. Charavaryamath ◽  
J. Wei ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Negative Impact ◽  
Clinical Applications ◽  
Regenerative Therapy ◽  
Defect Model ◽  
Chitosan Films ◽  
Cells Cultured

Chitosan opens new perspectives in regenerative medicine as it enhances the properties of mesenchymal stem cells (MSCs) through formation of spheroids. Hypoxia has also been proposed to enhance stemness and survival of MSCs afterin vivoimplantation. These characteristics are relevant to the development of an off-the-shelf source of allogenic cells for regenerative therapy of tendinopathies. Umbilical cord-derived MSCs (UCM-MSCs) offer an abundant source of immature and immunoprivileged stem cells. In this study, equine UCM-MSCs (eqUCM-MSCs) conditioned for 3 and 7 days on chitosan films at 5% oxygen were compared to eqUCM-MSCs under standard conditions. Equine UCM-MSCs formed spheroids on chitosan but yielded 72% less DNA than standard eqUCM-MSCs. Expression ofSox2,Oct4, andNanogwas 4 to 10 times greater in conditioned cells at day 7. Fluorescence-labeled cells cultured for 7 days under standard conditions or on chitosan films under hypoxia were compared in a bilateral patellar tendon defect model in rats. Fluorescence was present in all treated tendons, but the modulus of elasticity under tension was greater in tendons treated with conditioned cells. Chitosan and hypoxia affected cell yield but improved the stemness of eqUCM-MSCs and their contribution to the healing of tissues. Given the abundance of allogenic cells, these properties are highly relevant to clinical applications and outweigh the negative impact on cell proliferation.

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