Differential expression of connexin 43 in gastrointestinal stromal tumours of gastric and small intestinal origin

2005 ◽  
Vol 206 (4) ◽  
pp. 377-382 ◽  
Author(s):  
Akiko Nishitani ◽  
Seiichi Hirota ◽  
Toshirou Nishida ◽  
Koji Isozaki ◽  
Keiko Hashimoto ◽  
...  
Keyword(s):  
Differential Expression ◽  
Connexin 43 ◽  
Gastrointestinal Stromal Tumours ◽  
Intestinal Origin ◽  
Small Intestinal

scholarly journals The Differential Expression of Myometrial Connexin-43, Cyclooxygenase-1 and -2, and Gsα Proteins in the Upper and Lower Segments of the Human Uterus during Pregnancy and Labor1

1999 ◽  
Vol 84 (5) ◽  
pp. 1705-1710 ◽  
Author(s):  
Colette Sparey ◽  
Stephen C. Robson ◽  
Jarrod Bailey ◽  
Fiona Lyall ◽  
G. Nicholas Europe-Finner
Keyword(s):  
Gap Junction ◽  
Differential Expression ◽  
Connexin 43 ◽  
Cervical Ripening ◽  
Human Uterus ◽  
Down Regulation ◽  
Specific Antibodies ◽  
Cyclooxygenase 1 ◽  
Associated Proteins ◽  
Cox 1

There is evidence from many studies indicating that a number of specific quiescent and contractile associated proteins are temporally regulated in the myometrium during pregnancy. In this present investigation we provide data that strongly suggest that myometrial connexin-43, cyclooxygenase-1 and -2 (COX-1 and -2), and Gsα proteins are also spatially expressed within the human uterus during pregnancy and labor. Using paired lower and upper segment myometrial samples taken from individual women at term and during spontaneous labor, we have measured the expression of these proteins by immunoblotting with specific antibodies. We report that the myometrial gap junction connexin-43 protein is expressed at much greater levels in the upper uterine compared to the lower uterine segment and that this difference is even more pronounced during the course of labor. Conversely, myometrial COX-1 and -2 proteins appear to be expressed at much greater levels in the lower compared to the upper uterine segment. Moreover, the level of expression of both proteins is unaffected by the onset of parturition. In contrast, myometrial Gsα protein appears to be uniformly expressed in both lower and upper segments and is similarly down-regulated during parturition, as previously reported. The differential expression of COX-1 and -2 and connexin-43 in the uterus may allow cervical ripening before and dilatation during labor and facilitate effective propagation of contractions from fundus to cervix, which may be further facilitated by the down-regulation of Gsα at the onset of parturition.

Differential expression of connexin 43 in the chick tangential vestibular nucleus

2003 ◽  
Vol 71 (5) ◽  
pp. 617-628 ◽  
Author(s):  
Anastas Popratiloff ◽  
Seth M. Pollack ◽  
Christian Giaume ◽  
Kenna D. Peusner
Keyword(s):  
Differential Expression ◽  
Connexin 43 ◽  
Vestibular Nucleus

Differential Expression of Connexin 43 in Adult Pig Testes During Normal Spermatogenic Cycle and After Flutamide Treatment

2011 ◽  
Vol 46 (6) ◽  
pp. 1050-1060 ◽  
Author(s):  
I Kopera ◽  
M Durlej ◽  
A Hejmej ◽  
K Knapczyk-Stwora ◽  
M Duda ◽  
...  
Keyword(s):  
Differential Expression ◽  
Connexin 43

Differential expression of connexin 43 in mouse mammary cells

2006 ◽  
Vol 30 (5) ◽  
pp. 472-479 ◽  
Author(s):  
T LAMBE ◽  
D FINLAY ◽  
M MURPHY ◽  
F MARTIN
Keyword(s):  
Differential Expression ◽  
Connexin 43 ◽  
Mammary Cells

scholarly journals Identification of Bacterial Isolates Obtained from Intestinal Contents Associated with 12,000-Year-Old Mastodon Remains

1998 ◽  
Vol 64 (2) ◽  
pp. 651-658 ◽  
Author(s):  
A. N. Rhodes ◽  
J. W. Urbance ◽  
H. Youga ◽  
H. Corlew-Newman ◽  
C. A. Reddy ◽  
...  
Keyword(s):  
Soft Tissues ◽  
Anaerobic Bacteria ◽  
Acid Methyl Ester ◽  
Acid Methyl ◽  
Rdna Sequencing ◽  
The Family ◽  
Intestinal Contents ◽  
Intestinal Origin ◽  
Small Intestinal ◽  
Mammut Americanum

ABSTRACT Mastodon (Mammut americanum) remains unearthed during excavation of ancient sediments usually consist only of skeletal material, due to postmortem decomposition of soft tissues by microorganisms. Two recent excavations of skeletal remains in anoxic sediments in Ohio and Michigan, however, have uncovered organic masses which appear to be remnants of the small and large intestines, respectively. Macrobotanical examinations of the composition of these masses revealed assemblages of plant material radiocarbon dated to approximately 11,500 years before the present and thought to be incompletely digested food remains from this extinct mammal. We attempted to cultivate and identify bacteria from the intestinal contents, bone-associated sediments, and sediments not in proximity to the remains using a variety of general and selective media. In all, 295 isolates were cultivated, and 38 individual taxa were identified by fatty acid-methyl ester (FAME) profiles and biochemical characteristics (API-20E). The taxonomic positions of selected enteric and obligately anaerobic bacteria were confirmed by 16S ribosomal DNA (rDNA) sequencing. Results indicate that the intestinal and bone-associated samples contained the greatest diversity of bacterial taxa and that members of the family Enterobacteriaceae represented 41% of all isolates and were predominant in the intestinal masses and sediments in proximity to the skeleton but were uncommon in the background sediments. Enterobacter cloacae was the most commonly identified isolate, and partial rDNA sequencing revealed thatRahnella aquatilis was the correct identity of strains suggested by FAME profiles to be Yersinia enterocolitica. No Bacteroides spp. or expected intestinal anaerobes were recovered. The only obligate anaerobes recovered were clostridia, and these were not recovered from the small intestinal masses. Microbiological evidence from this study supports other, macrobotanical data indicating the intestinal origin of these masses. Whether these organisms are direct descendants of the original intestinal microbiota, however, cannot be established.

Unilateral Effects of Pregnancy on Differential Expression of the Gap Junction Proteins Connexin 43 and 37 in Ovine Uterine Artery Endothelium.

2010 ◽  
Vol 83 (Suppl_1) ◽  
pp. 477-477
Author(s):  
Timothy J. Morschauser ◽  
Jayanth Ramadoss ◽  
Jill M. Koch ◽  
Gladys E. Lopez ◽  
Ian M. Bird ◽  
...  
Keyword(s):  
Gap Junction ◽  
Differential Expression ◽  
Connexin 43 ◽  
Uterine Artery ◽  
Unilateral Effects ◽  
Gap Junction Proteins ◽  
Junction Proteins

EXPECT THE UNEXPECTED: JEJUNAL GASTROINTESTINAL TUMOUR MIMICKING OVARIAN MALIGNANCY- A CASE REPORT WITH REVIEW OF LITERATURE

2021 ◽  
pp. 12-13
Author(s):  
Shivangi Singh ◽  
Munesh Munesh ◽  
Sweta Sweta
Keyword(s):  
Gastrointestinal Stromal Tumors ◽  
Alimentary Tract ◽  
Ovarian Malignancy ◽  
Review Of Literature ◽  
Gastrointestinal Stromal Tumours ◽  
Stromal Tumors ◽  
Epitheloid Cells ◽  
Missed Diagnosis ◽  
Small Intestinal ◽  
Mesenchymal Tumours

Gastrointestinal stromal tumours (GISTs) are mesenchymal tumours of alimentary tract comprising 0.2% of gastrointestinal tumors and only 0.04% of small intestinal tumours. Jejunal GISTs are one of the rarest subtypes. GISTs display various morphological forms like spindle and epitheloid cells in a variety of patterns and can be submucosal, intramuscular or subserosal in location. Grossly they are solid and cyst with variable hemorrhage and necrosis. Most of the gastrointestinal stromal tumors have mutations in either KIT (CD117) or PDGFRα gene. DOG 1 is a sensitive and specic marker of GIST independent of CD117 or PGDFRα expression. Here we present a case of malignant jejunal GIST with missed diagnosis on CECT abdomen.

Expression of gap junction connexin 43 and connexin 43 mRNA in different regional tissues of intestine in dog

1993 ◽  
Vol 265 (5) ◽  
pp. G911-G916 ◽  
Author(s):  
Z. Li ◽  
Z. Zhou ◽  
E. E. Daniel
Keyword(s):  
Gap Junction ◽  
Northern Blot ◽  
Connexin 43 ◽  
Longitudinal Muscle ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Circular Muscle ◽  
Northern Blot Hybridization ◽  
Junction Protein ◽  
Small Intestinal

To determine the distribution of gap junction protein connexin 43 (Cx43) and the expression of Cx43 mRNA in different regions of canine small intestine and colon, modified Western blot and Northern blot techniques were used with a site-directed antibody raised against a synthetic peptide of Cx43 Anti-Cx43 (252-271) and a probe of 1.3-kb Cx43 cDNA. Equal amounts of plasma membrane enriched fraction (10 micrograms protein) from small intestinal and colonic circular muscle, longitudinal muscle, and colonic submucous plexus border of circular muscle (interstitial cells of Cajal rich layer, ICC) of the dog were resolved by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred onto nitrocellulose, and blotted by chemiluminescent assay. Different yet characteristic ratios of the immunoreactive gap junction proteins located at 43 and 40 kDa were found in membranes from small intestinal and colonic circular smooth muscle, and ICC. These results suggest that gap junction Cx43 in dog heart, dog small intestinal and colonic circular muscle, and ICC is homologous to rat heart. Furthermore, with the use of Northern blot hybridization with a 1.3-kb Cx43 cDNA, a 3.0-kb message was observed in small intestinal and colonic circular muscles, longitudinal muscles, and ICC. However, the mRNA signal of small intestinal circular muscle was the strongest and that of longitudinal muscle was weakest, especially from colon longitudinal muscle.(ABSTRACT TRUNCATED AT 250 WORDS)

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