A transgenic mouse expressing miR‐210 in proximal tubule cells shows mitochondrial alteration: possible association of miR‐210 with a shift in energy metabolism

2020 ◽  
Vol 251 (1) ◽  
pp. 12-25 ◽  
Author(s):  
Chisato Nakada ◽  
Naoki Hijiya ◽  
Yoshiyuki Tsukamoto ◽  
Shinji Yano ◽  
Tomoki Kai ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Proximal Tubule ◽  
Transgenic Mouse ◽  
Proximal Tubule Cells ◽  
Mitochondrial Alteration ◽  
Tubule Cells

scholarly journals MO331LINEAGE TRACING OF REGENERATING PROXIMAL TUBULE CELLS (STC) BY SINGLE CELL PROFILING IN ACUTE KIDNEY INJURY

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Eleni Stamellou ◽  
Mingbo Cheng ◽  
Viktor Sterzer ◽  
Katja Leuchtle ◽  
Thiago Strieder ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Proximal Tubule ◽  
Single Cell ◽  
Transgenic Mouse ◽  
Ischemia Reperfusion ◽  
Expression Patterns ◽  
Kidney Injury ◽  
Multiple Time ◽  
Proximal Tubule Cells ◽  
Tubule Cells

Abstract Background and Aims Acute tubular injury accounts for the most common intrinsic cause for acute kidney injury (AKI). The scattered tubular cell (STC) phenotype was discovered as a uniform reaction of tubule cells triggered by injury. Our group was the first to identify an inducible transgenic mouse (PEC-rtTA-mouse) specifically labeling STCs with eGFP. Analysis of the transcriptional factors and associated signaling pathways might reveal the function and role of STCs in AKI. Method Here, we performed single-cell RNA sequencing of unilateral ischemia-reperfusion murine model of AKI 8, 24, 48 hours and 6 and 12 days after AKI induction. Results Genes expressing proximal tubular proteins and transporters were markedly downregulated during transition into the STC phenotype upon injury; but expression recovered over time and upon resolution and tubular cells re-differentiated into proximal tubule cells. This provides evidence for the first time that the STC phenotype is a transient and reversible phenotype triggered by injury. Among cells in the STC phenotype, we could identify 2 sub-clusters; a highly proliferating sub-cluster that in the cell cycle analysis showed the highest proportion of cycling cells. The second eGFP-positive cluster appeared very early after AKI and expressed a distinct set of genes (defined by 7 anchor genes). Some of the highly up-regulated genes are known markers of STCs hence confirming the specificity of our transgenic mouse line. Conclusion Our study provides gene expression patterns specifically in STCs upon injury and repair at multiple time points and suggests that the STC phenotype is a transient and reversible phenotype triggered by injury.

Nephrotoxicity Testing In Vitro: The Current Situation

1997 ◽  
Vol 25 (5) ◽  
pp. 497-503
Author(s):  
Jean-Paul Morin ◽  
Marc E. De Broe ◽  
Walter Pfaller ◽  
Gabriele Schmuck
Keyword(s):  
Proximal Tubule ◽  
Culture Media ◽  
Task Force ◽  
Primary Cultures ◽  
Phenotypic Expression ◽  
Transepithelial Transport ◽  
Specific Cell ◽  
Proximal Tubule Cells ◽  
Tubule Cells

An ECVAM task force on nephrotoxicity has been established to advise, in particular, on the follow-up to recommendations made in the ECVAM workshop report on nephrotoxicity testing in vitro. Since this workshop was held, in 1994, there have been several improvements in the techniques used. For example, the duration of renal slice viability, and the maintenance of functional activities in slices, have been improved by using dynamic incubation systems with higher oxygen tensions and more-appropriate cell culture media. Highly differentiated primary cultures of pig, human and rabbit proximal tubule cells have been established by using specific cell isolation procedures and/or selective culture media. To date, the most comparable phenotypic expression and transepithelial transport capacities to proximal tubules in vivo have been obtained with primary cultures of rabbit proximal tubule cells which are grown on bicompartmental supports; in this system, transepithelial substrate gradients are generated and the transepithelial transport of both organic anions and cations is highly active. This in vitro system has been selected by ECVAM for further evaluation and prevalidation. Industrial needs in the area of nephrotoxicity testing have been identified, and recommendations are made at the end of this report concerning possible future initiatives.

Influence of vitamin D treatment on functional expression of drug disposition pathways in human kidney proximal tubule cells during simulated uremia

Xenobiotica ◽  
2021 ◽  
pp. 1-11
Author(s):  
Stacey M. Tuey ◽  
Amandla Atilano-Roque ◽  
Georgia Charkoftaki ◽  
Joshua M. Thurman ◽  
Thomas D. Nolin ◽  
...  
Keyword(s):  
Vitamin D ◽  
Proximal Tubule ◽  
Drug Disposition ◽  
Human Kidney ◽  
Functional Expression ◽  
Proximal Tubule Cells ◽  
Kidney Proximal Tubule ◽  
Tubule Cells

Effects of TCDD and estradiol-17β on the proliferation and Na+/glucose cotransporter in renal proximal tubule cells

2005 ◽  
Vol 19 (1) ◽  
pp. 21-30 ◽  
Author(s):  
Ho Jae Han ◽  
Min Jin Lim ◽  
Yun Jung Lee ◽  
Eun Jung Kim ◽  
Young Jin Jeon ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Renal Proximal Tubule Cells ◽  
Estradiol 17Β

scholarly journals Electrophysiology and ultrastructure of cultured human proximal tubule cells

1988 ◽  
Vol 33 (2) ◽  
pp. 508-516 ◽  
Author(s):  
John G. Blackburn ◽  
Debra J. Hazen-Martin ◽  
Carol J. Detrisac ◽  
Donald A. Sens
Keyword(s):  
Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Human Proximal Tubule

scholarly journals Aldosterone regulates Na+, K+ ATPase activity in human renal proximal tubule cells through mineralocorticoid receptor

2013 ◽  
Vol 1833 (10) ◽  
pp. 2143-2152 ◽  
Author(s):  
Sarah A. Salyer ◽  
Jason Parks ◽  
Michelle T. Barati ◽  
Eleanor D. Lederer ◽  
Barbara J. Clark ◽  
...  
Keyword(s):  
Atpase Activity ◽  
Proximal Tubule ◽  
Mineralocorticoid Receptor ◽  
Renal Proximal Tubule ◽  
Proximal Tubule Cells ◽  
Tubule Cells ◽  
Renal Proximal Tubule Cells

Distinct interleukin-1β-converting enzyme family proteases mediate cisplatin- and staurosporine-induced apoptosis of mouse proximal tubule cells

Life Sciences ◽  
1998 ◽  
Vol 62 (12) ◽  
pp. 1125-1138 ◽  
Author(s):  
Kazuhito Fukuoka ◽  
Michio Takeda ◽  
Mami Kobayashi ◽  
Takako Osaki ◽  
Isao Shiratc ◽  
...  
Keyword(s):  
Proximal Tubule ◽  
Interleukin 1Β ◽  
Converting Enzyme ◽  
Proximal Tubule Cells ◽  
Enzyme Family ◽  
Tubule Cells ◽  
Induced Apoptosis
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