scholarly journals Quantitative confocal imaging method for analyzing cellulose dynamics during cell wall regeneration in Arabidopsis mesophyll protoplasts

Plant Direct ◽  
2017 ◽  
Vol 1 (6) ◽  
pp. e00021 ◽  
Author(s):  
Hiroaki Kuki ◽  
Takumi Higaki ◽  
Ryusuke Yokoyama ◽  
Takeshi Kuroha ◽  
Naoki Shinohara ◽  
...  
Keyword(s):  
Cell Wall ◽  
Confocal Imaging ◽  
Imaging Method ◽  
Cell Wall Regeneration ◽  
Mesophyll Protoplasts ◽  
Wall Regeneration

Factors that Influence the Yield, Stability in Culture and Cell Wall Regeneration of Spinach Mesophyll Protoplasts

1980 ◽  
Vol 7 (6) ◽  
pp. 713 ◽  
Author(s):  
R.J Rose
Keyword(s):  
Cell Wall ◽  
Cell Walls ◽  
Yield Stability ◽  
Developmental Studies ◽  
Cell Wall Regeneration ◽  
Mesophyll Cells ◽  
Isolation Medium ◽  
Mesophyll Protoplasts ◽  
Wall Regeneration ◽  
Ability To Regenerate

A study has been made of factors that influence the yield, stability in culture, and ability to regenerate cell walls of isolated spinach (Spinacia olevacea L.) mesophyll protoplasts. The presence of 7 mM CaZ + or 7 mM Mg2+ in the isolation medium, which also included 1.5 % (w/v) Driselase, 0.25 % (w/v) pectinase and 0.8 M sorbitol, increased the yield and stability in culture of the protoplasts in a liquid nutrient medium. This latter medium has been used previously in the culture of spinach leaf discs, and does not support the division of spinach mesophyll cells. Protoplasts isolated in the presence of CaZ+ showed a greater capacity for cell wall regeneration compared with protoplasts isolated in the absence of ions or in the presence of Mg2+. Though darkness during culture improved the initial protoplast stability, it inhibited wall regeneration. The initial stability of protoplasts appeared to be dependent on plasma membrane stability, but after a few days in culture the most effective treatments were those which stimulated cell wall regeneration. In many cases, associated with cell wall regeneration, there was a budding off of small vesicles which sometimes contained chloroplasts. Protoplasts isolated in the presence of Ca2+ and incubated in low light had a 50% survival rate after 8 days culture and most had regenerated cell walls. Such culture of spinach protoplasts has not been shown previously and should be useful in developmental studies of spinach chloroplasts.

Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts

Planta ◽  
1970 ◽  
Vol 92 (4) ◽  
pp. 301-308 ◽  
Author(s):  
Toshiyuki Nagata ◽  
Itaru Takebe
Keyword(s):  
Cell Wall ◽  
Cell Division ◽  
Cell Wall Regeneration ◽  
Mesophyll Protoplasts ◽  
Wall Regeneration

Modification of protoplast cell wall regeneration by membrane perturbation

PROTOPLASMA ◽  
1988 ◽  
Vol 143 (1) ◽  
pp. 38-42 ◽  
Author(s):  
R. L. Legge ◽  
R. M. Brown
Keyword(s):  
Cell Wall ◽  
Cell Wall Regeneration ◽  
Wall Regeneration ◽  
Membrane Perturbation

Cell Wall Regeneration and Protoplast Reversion

2020 ◽  
pp. 115-133
Author(s):  
Oldřich Nečas ◽  
Augustin Svoboda
Keyword(s):  
Cell Wall ◽  
Cell Wall Regeneration ◽  
Wall Regeneration

Ultrastructural Observations of Cell Wall Regeneration Around Isolated Tobacco Protoplasts

1974 ◽  
Vol 14 (2) ◽  
pp. 439-449
Author(s):  
J. BURGESS ◽  
E. N. FLEMING
Keyword(s):  
Electron Microscopy ◽  
Heavy Metal ◽  
Endoplasmic Reticulum ◽  
Cell Wall ◽  
Cell Wall Regeneration ◽  
Membrane Systems ◽  
Tobacco Protoplasts ◽  
Wall Formation ◽  
Wall Regeneration ◽  
Wall Growth

The process of cell wall regeneration around cultured protoplasts isolated from tobacco mesophyll has been examined by electron microscopy. The initially formed wall contains 2 components which stain with conventional heavy metal stains. The first consists of un-branched fibres, at first oriented at right angles to the plasmalemma surface. As wall growth proceeds the fibres lengthen and assume an orientation parallel to the plasmalemma. It seems probable that this component is cellulose. The second component of the wall is more amorphous and more densely stained. It is most frequently seen in situations where leaching of materials into the medium would be expected to be minimal. The endoplasmic reticulum and the plasmalemma are the only membrane systems which appear to contribute towards wall formation. No pattern of structure has been detected to explain the orientation or method of synthesis of the microfibrillar part of the wall.

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