The Effects of Extracellular Calmodulin on Cell Wall Regeneration of Protoplasts and Cell Division

1995 ◽  
Keyword(s):  
Cell Wall ◽  
Cell Division ◽  
Cell Wall Regeneration ◽  
Wall Regeneration ◽  
Extracellular Calmodulin

Protoplast isolation and fusion in three Ulmus species

1981 ◽  
Vol 59 (8) ◽  
pp. 1436-1443 ◽  
Author(s):  
Keith Redenbaugh ◽  
David F. Karnosky ◽  
Robert D. Westfall
Keyword(s):  
Cell Wall ◽  
Cell Division ◽  
Protoplast Isolation ◽  
Suspension Cultures ◽  
Cell Wall Regeneration ◽  
Intact Cells ◽  
Ulmus Americana ◽  
Wall Regeneration

Protoplasts were isolated from cotyledons, callus, and suspension cultures of Ulmus americana L. and U. pumula L. and from cotyledons and callus of U. parvifolia Jacq. using various enzyme solutions. Isolation frequencies (percent protoplasts in a solution containing both protoplasts and broken or damaged protoplasts, but very few intact cells) were usually about 10%; however, for U. pumila cotyledons, protoplast frequencies reached 100%. In a limited number of cases, cell wall regeneration occurred after 4–21 days and cell division after 9–21 days. Finally, U. pumila protoplasts were fused using a PEG–calcium solution.

scholarly journals Quantitative confocal imaging method for analyzing cellulose dynamics during cell wall regeneration in Arabidopsis mesophyll protoplasts

Plant Direct ◽  
2017 ◽  
Vol 1 (6) ◽  
pp. e00021 ◽  
Author(s):  
Hiroaki Kuki ◽  
Takumi Higaki ◽  
Ryusuke Yokoyama ◽  
Takeshi Kuroha ◽  
Naoki Shinohara ◽  
...  
Keyword(s):  
Cell Wall ◽  
Confocal Imaging ◽  
Imaging Method ◽  
Cell Wall Regeneration ◽  
Mesophyll Protoplasts ◽  
Wall Regeneration

Cell Wall Regeneration and Protoplast Reversion

2020 ◽  
pp. 115-133
Author(s):  
Oldřich Nečas ◽  
Augustin Svoboda
Keyword(s):  
Cell Wall ◽  
Cell Wall Regeneration ◽  
Wall Regeneration

Ultrastructural Observations of Cell Wall Regeneration Around Isolated Tobacco Protoplasts

1974 ◽  
Vol 14 (2) ◽  
pp. 439-449
Author(s):  
J. BURGESS ◽  
E. N. FLEMING
Keyword(s):  
Electron Microscopy ◽  
Heavy Metal ◽  
Endoplasmic Reticulum ◽  
Cell Wall ◽  
Cell Wall Regeneration ◽  
Membrane Systems ◽  
Tobacco Protoplasts ◽  
Wall Formation ◽  
Wall Regeneration ◽  
Wall Growth

The process of cell wall regeneration around cultured protoplasts isolated from tobacco mesophyll has been examined by electron microscopy. The initially formed wall contains 2 components which stain with conventional heavy metal stains. The first consists of un-branched fibres, at first oriented at right angles to the plasmalemma surface. As wall growth proceeds the fibres lengthen and assume an orientation parallel to the plasmalemma. It seems probable that this component is cellulose. The second component of the wall is more amorphous and more densely stained. It is most frequently seen in situations where leaching of materials into the medium would be expected to be minimal. The endoplasmic reticulum and the plasmalemma are the only membrane systems which appear to contribute towards wall formation. No pattern of structure has been detected to explain the orientation or method of synthesis of the microfibrillar part of the wall.

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