High-accuracy identification and bioinformatic analysis of in vivo protein phosphorylation sites in yeast

PROTEOMICS ◽  
2009 ◽  
Vol 9 (20) ◽  
pp. 4642-4652 ◽  
Author(s):  
Florian Gnad ◽  
Lyris M. F. de Godoy ◽  
Jürgen Cox ◽  
Nadin Neuhauser ◽  
Shubin Ren ◽  
...  
Keyword(s):  
Protein Phosphorylation ◽  
Bioinformatic Analysis ◽  
High Accuracy ◽  
Phosphorylation Sites

scholarly journals Analysis of Flagellar Phosphoproteins from Chlamydomonas reinhardtii

2009 ◽  
Vol 8 (7) ◽  
pp. 922-932 ◽  
Author(s):  
Jens Boesger ◽  
Volker Wagner ◽  
Wolfram Weisheit ◽  
Maria Mittag
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Protein Phosphorylation ◽  
Proteome Analysis ◽  
Physiological Status ◽  
Phosphorylation Sites ◽  
Cell Organelles ◽  
Flagellar Proteins ◽  
Reversible Protein Phosphorylation ◽  
Cilia And Flagella

ABSTRACT Cilia and flagella are cell organelles that are highly conserved throughout evolution. For many years, the green biflagellate alga Chlamydomonas reinhardtii has served as a model for examination of the structure and function of its flagella, which are similar to certain mammalian cilia. Proteome analysis revealed the presence of several kinases and protein phosphatases in these organelles. Reversible protein phosphorylation can control ciliary beating, motility, signaling, length, and assembly. Despite the importance of this posttranslational modification, the identities of many ciliary phosphoproteins and knowledge about their in vivo phosphorylation sites are still missing. Here we used immobilized metal affinity chromatography to enrich phosphopeptides from purified flagella and analyzed them by mass spectrometry. One hundred forty-one phosphorylated peptides were identified, belonging to 32 flagellar proteins. Thereby, 126 in vivo phosphorylation sites were determined. The flagellar phosphoproteome includes different structural and motor proteins, kinases, proteins with protein interaction domains, and many proteins whose functions are still unknown. In several cases, a dynamic phosphorylation pattern and clustering of phosphorylation sites were found, indicating a complex physiological status and specific control by reversible protein phosphorylation in the flagellum.

Identification of in vivo protein phosphorylation sites in human pathogen Schistosoma japonicum by a phosphoproteomic approach

2012 ◽  
Vol 75 (3) ◽  
pp. 868-877 ◽  
Author(s):  
Rong Luo ◽  
Chunjing Zhou ◽  
Jiaojiao Lin ◽  
Dehao Yang ◽  
Yaojun Shi ◽  
...  
Keyword(s):  
Protein Phosphorylation ◽  
Schistosoma Japonicum ◽  
Phosphorylation Sites ◽  
Human Pathogen

scholarly journals PhosphoGRID: a database of experimentally verified in vivo protein phosphorylation sites from the budding yeast Saccharomyces cerevisiae

Database ◽  
2010 ◽  
Vol 2010 (0) ◽  
pp. bap026-bap026 ◽  
Author(s):  
C. Stark ◽  
T.-C. Su ◽  
A. Breitkreutz ◽  
P. Lourenco ◽  
M. Dahabieh ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Phosphorylation ◽  
Budding Yeast ◽  
Phosphorylation Sites ◽  
Yeast Saccharomyces Cerevisiae

scholarly journals In vivo phosphorylation sites in fetal and adult rat tau.

1993 ◽  
Vol 268 (34) ◽  
pp. 25712-25717
Author(s):  
A Watanabe ◽  
M Hasegawa ◽  
M Suzuki ◽  
K Takio ◽  
M Morishima-Kawashima ◽  
...  
Keyword(s):  
Phosphorylation Sites ◽  
Adult Rat

scholarly journals Genome wide effects of oleic acid on cultured bovine granulosa cells: evidence for the activation of pathways favoring folliculo-luteal transition

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Vengala Rao Yenuganti ◽  
Dirk Koczan ◽  
Jens Vanselow
Keyword(s):  
Oleic Acid ◽  
Granulosa Cells ◽  
System Development ◽  
Circulatory System ◽  
Bioinformatic Analysis ◽  
Negative Energy ◽  
Tissue Remodelling ◽  
Gonadotropin Secretion ◽  
Genome Wide

Abstract Background Metabolic stress, as negative energy balance on one hand or obesity on the other hand can lead to increased levels of free fatty acids in the plasma and follicular fluid of animals and humans. In an earlier study, we showed that increased oleic acid (OA) concentrations affected the function of cultured bovine granulosa cells (GCs). Here, we focus on genome wide effects of increased OA concentrations. Results Our data showed that 413 genes were affected, of which 197 were down- and 216 up-regulated. Specifically, the expression of FSH-regulated functional key genes, CCND2, LHCGR, INHA and CYP19A1 and 17-β-estradiol (E2) production were reduced by OA treatment, whereas the expression of the fatty acid transporter CD36 was increased and the morphology of the cells was changed due to lipid droplet accumulation. Bioinformatic analysis revealed that associated pathways of the putative upstream regulators “FSH” and “Cg (choriogonadotropin)” were inhibited and activated, respectively. Down-regulated genes are over-represented in GO terms “reproductive structure/system development”, “ovulation cycle process”, and “(positive) regulation of gonadotropin secretion”, whereas up-regulated genes are involved in “circulatory system development”, “vasculature development”, “angiogenesis” or “extracellular matrix/structure organization”. Conclusions From these data we conclude that besides inhibiting GC functionality, increased OA levels seemingly promote angiogenesis and tissue remodelling, thus suggestively initiating a premature fulliculo-luteal transition. In vivo this may lead to impeded folliculogenesis and ovulation, and cause sub-fertility.

scholarly journals Identification of multiple in vivo phosphorylation sites in rabbit myelin basic protein.

1983 ◽  
Vol 258 (2) ◽  
pp. 930-937 ◽  
Author(s):  
R E Martenson ◽  
M J Law ◽  
G E Deibler
Keyword(s):  
Myelin Basic Protein ◽  
Basic Protein ◽  
Phosphorylation Sites

scholarly journals Frequency- and Afterload-Dependent Cardiac Modulation In Vivo by Troponin I With Constitutively Active Protein Kinase A Phosphorylation Sites

2004 ◽  
Vol 94 (4) ◽  
pp. 496-504 ◽  
Author(s):  
Eiki Takimoto ◽  
David G. Soergel ◽  
Paul M.L. Janssen ◽  
Linda B. Stull ◽  
David A. Kass ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Troponin I ◽  
Phosphorylation Sites ◽  
Active Protein ◽  
Cardiac Modulation ◽  
Kinase A ◽  
Constitutively Active
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