Data‐Independent Acquisition‐Based Quantitative Proteomics Analysis Reveals Dynamic Network Profiles during the Macrophage Inflammatory Response

PROTEOMICS ◽  
2020 ◽  
Vol 20 (2) ◽  
pp. 1900203 ◽  
Author(s):  
Lei Li ◽  
Li Chen ◽  
Xinya Lu ◽  
Chenyang Huang ◽  
Haihua Luo ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Quantitative Proteomics ◽  
Dynamic Network ◽  
Proteomics Analysis ◽  
Data Independent Acquisition

scholarly journals A proteomic perspective and involvement of cytokines in SARS-CoV-2 infection

2021 ◽  
Author(s):  
Sarena Banu ◽  
Mohammed M Idris ◽  
Ramakrishnan Nagaraj
Keyword(s):  
Inflammatory Response ◽  
Quantitative Proteomics ◽  
Immune Cell ◽  
Cell Trafficking ◽  
Cytokine Storm ◽  
Proteomics Analysis ◽  
Initial Infection ◽  
Clinical Observations ◽  
Normal Individuals ◽  
Oropharyngeal Swab

Infection with the SARS-CoV-2 virus results in manifestation of several clinical observations from asymptomatic to multi-organ failure. Biochemically, the serious effects are due to what is described as cytokine storm. The initial infection region for COVID-19 is the nasopharyngeal/oropharyngeal region which is the site where samples are taken to examine the presence of virus. We have earlier shown that several defensin genes are down regulated in cells from this region in patients who tested positive in the RTPCR test. We have now carried out detailed proteomic analysis of the nasopharyngeal/oropharyngeal swab samples collected from normal individuals and those tested positive for SARS-CoV-2 by RTPCR, involving high throughput quantitative proteomics analysis. Several proteins like annexins, cytokines and histones were found differentially regulated in the host human cells following SARS-CoV-2 infection. Genes for these proteins were also observed to be differentially regulated when their expression was analyzed. Majority of the cytokine proteins were found to be up regulated in the infected individuals. Cell to Cell signaling interaction, Immune cell trafficking and inflammatory response pathways were found associated with the differentially regulated proteins based on network pathway analysis.

Quantitative Proteomics Analysis Reveals Unique But Overlapping Protein Signatures in HIV Infection

2019 ◽  
Author(s):  
Maha Al-Mozaini ◽  
Ibtihag S. Alsharif ◽  
Al-Hussain J. Alzahrani ◽  
Zakia Shinwari ◽  
Magid Halim ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Quantitative Proteomics ◽  
Proteomics Analysis ◽  
Protein Signatures

scholarly journals Quantitative Proteomics Analysis Reveals Unique but Overlapping Protein Signatures in HIV Infections

2021 ◽  
Author(s):  
Maha Al-Mozaini ◽  
Ibtihag Alsharif ◽  
Alhusain Alzahrani ◽  
Zakia Shinwari ◽  
Magid Halim ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Hiv Infections ◽  
Proteomics Analysis ◽  
Protein Signatures

A Gel-Free Quantitative Proteomics Analysis of Factors Released From Hypoxic-Conditioned Placentae

2009 ◽  
Vol 17 (3) ◽  
pp. 247-257 ◽  
Author(s):  
Richard T. Blankley ◽  
Nicola J. Robinson ◽  
John D. Aplin ◽  
Ian P. Crocker ◽  
Simon J. Gaskell ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Proteomics Analysis

scholarly journals Quantitative proteomics analysis reveals similar release profiles following specific PAR-1 or PAR-4 stimulation of platelets

2014 ◽  
Vol 103 (1) ◽  
pp. 140-146 ◽  
Author(s):  
Thijs C. van Holten ◽  
Onno B. Bleijerveld ◽  
Patrick Wijten ◽  
Philip G. de Groot ◽  
Albert J.R. Heck ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Proteomics Analysis ◽  
Stimulation Of ◽  
Release Profiles

Quantitative proteomics analysis reveals novel insights into mechanisms of action of disulfiram (DSF)

2021 ◽  
pp. 2100031
Author(s):  
Peng Zheng ◽  
Chenglinzi Liu ◽  
Yaoqin Wu ◽  
Ruifeng Xu ◽  
Ying Chen ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Mechanisms Of Action ◽  
Proteomics Analysis

scholarly journals Comparative Proteomics Analysis of Mouse Habu Nephritis Models with and without Unilateral Nephrectomy

2016 ◽  
Vol 39 (5) ◽  
pp. 1761-1776 ◽  
Author(s):  
Lei Chen ◽  
Yang Lu ◽  
Jun Wen ◽  
Xu Wang ◽  
Lingling Wu ◽  
...  
Keyword(s):  
Renal Injury ◽  
Quantitative Proteomics ◽  
Mesangial Cells ◽  
Unilateral Nephrectomy ◽  
Label Free ◽  
Proteomics Analysis ◽  
Single Kidney ◽  
Regulation Of Actin Cytoskeleton ◽  
Insight Into

Background/Aims: Individuals possessing a single kidney are at greater risk of renal injury upon exposure to harmful stimuli. This study aimed to explore the pathogenesis of renal injury in glomerulonephritis with versus without unilateral nephrectomy (UNX). Methods: Histological analysis and label-free quantitative proteomics were performed on two models—the Habu snake venom-induced glomerulonephritis model with versus without UNX (HabuU and Habu models, respectively). The role of villin 1, a differentially expressed protein (DEP) in mouse mesangial cells, was investigated. Results: Persistent mesangiolysis and focal hypercellularity together with reduced activation of cell proliferation in the HabuU model induced more serious renal injury compared with that in the Habu model. The DEPs between the two models were identified by label-free liquid chromatography-mass spectrometry. The KEGG pathway results indicated that regulation of actin cytoskeleton and focal adhesion were specifically enriched in the HabuU model. The cytoskeleton regulation protein villin 1 was downregulated in the HabuU model, but unchanged in the Habu model. Knockdown of villin 1 promoted apoptosis and inhibited the proliferation of mouse mesangial cells, suggesting villin 1 to be involved in qlomerular lesion self-repair insufficiency. Conclusion: By assessing the proteomic profiles of the two models, this study identified several important differences, particularly villin 1 expression, in regulatory mechanisms between the two models. Our findings provide novel insight into the mechanism of serious renal injury in glomerulonephritis with UNX.

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