Clinical application of direct sputum sensitivity testing in a severe infective exacerbation of cystic fibrosis

2003 ◽  
Vol 35 (6) ◽  
pp. 463-466 ◽  
Author(s):  
David J. Serisier ◽  
Graeme Jones ◽  
Andrew Tuck ◽  
Gary Connett ◽  
Mary P. Carroll
Keyword(s):  
Cystic Fibrosis ◽  
Clinical Application ◽  
Sensitivity Testing

scholarly journals Establishment and Application of Rapid Diagnosis for Reverse Transcription-Quantitative PCR of Newly Emerging Goose-Origin Nephrotic Astrovirus in China

mSphere ◽  
2018 ◽  
Vol 3 (6) ◽  
Author(s):  
Xiaoyuan Yuan ◽  
Kai Meng ◽  
Yuxia Zhang ◽  
Lihong Qi ◽  
Wu Ai ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Clinical Application ◽  
Reverse Transcription ◽  
Test Sample ◽  
Sensitivity Testing ◽  
Content Type ◽  
Reverse Transcription Quantitative Pcr ◽  
Emerging Virus ◽  
New Type ◽  
And Control

ABSTRACT In 2017, a new type of goose-origin astrovirus (GoAstV) that is completely different from previously identified avian astroviruses (which have only 30.0% to 50.5% homology with GoAstV) has been isolated from diseased geese in China. This disease can cause joint swelling in sick geese, and the anatomy shows a clear precipitation of urate in the kidney. The rate of death and culling can reach more than 30%, revealing the disease’s severe pathogenicity. To quickly and accurately diagnose the newly emerging disease, we established a highly specific reverse transcription-quantitative PCR (RT-qPCR) method of detecting GoAstV. Sensitivity testing showed that the minimum amount of test sample for this method is 52.5 copies/μl. Clinical application confirmed that this method can quickly and effectively detect GoAstV, providing a diagnostic platform for the prevention and control of goose disease. IMPORTANCE Goose-origin astrovirus (GoAstV), as a newly emerging virus in 2017, is different from previously known astroviruses in the genus Avastrovirus. So far, few studies have focused on the novel virus. Considering the infectious development of astrovirus (AstV), we established a reverse transcription-quantitative PCR (RT-qPCR) assay with a strong specificity to quickly and accurately diagnose GoAstV. Confirmed by clinical application, this method can quickly and accurately detect prevalent GoAstV. The assay is thus convenient for clinical operation and is applicable to the monitoring of GoAstV disease.

Clinical Application of Stem/Stromal Cells in Cystic Fibrosis

2019 ◽  
pp. 179-198
Author(s):  
Steven T. Leung ◽  
Timothy S. Leach ◽  
Anthony Atala ◽  
Sean V. Murphy
Keyword(s):  
Cystic Fibrosis ◽  
Clinical Application ◽  
Stromal Cells

scholarly journals Polymicrobial Interactions in the Cystic Fibrosis Airway Microbiome Impact the Antimicrobial Susceptibility of Pseudomonas aeruginosa

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 827
Author(s):  
Emma Reece ◽  
Pedro H. de Almeida Bettio ◽  
Julie Renwick
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Airway Disease ◽  
Chronic Infections ◽  
Sensitivity Testing ◽  
Antimicrobial Sensitivity ◽  
Cystic Fibrosis Airway ◽  
Airway Microbiome ◽  
Diverse Community

Pseudomonas aeruginosa is one of the most dominant pathogens in cystic fibrosis (CF) airway disease and contributes to significant inflammation, airway damage, and poorer disease outcomes. The CF airway is now known to be host to a complex community of microorganisms, and polymicrobial interactions have been shown to play an important role in shaping P. aeruginosa pathogenicity and resistance. P. aeruginosa can cause chronic infections that once established are almost impossible to eradicate with antibiotics. CF patients that develop chronic P. aeruginosa infection have poorer lung function, higher morbidity, and a reduced life expectancy. P. aeruginosa adapts to the CF airway and quickly develops resistance to several antibiotics. A perplexing phenomenon is the disparity between in vitro antimicrobial sensitivity testing and clinical response. Considering the CF airway is host to a diverse community of microorganisms or ‘microbiome’ and that these microorganisms are known to interact, the antimicrobial resistance and progression of P. aeruginosa infection is likely influenced by these microbial relationships. This review combines the literature to date on interactions between P. aeruginosa and other airway microorganisms and the influence of these interactions on P. aeruginosa tolerance to antimicrobials.

Clinical application of preimplantation genetic diagnosis for cystic fibrosis

2000 ◽  
Vol 20 (7) ◽  
pp. 571-581 ◽  
Author(s):  
V. Goossens ◽  
K. Sermon ◽  
W. Lissens ◽  
M. Vandervorst ◽  
A. Vanderfaeillie ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Clinical Application ◽  
Preimplantation Genetic Diagnosis ◽  
Genetic Diagnosis

Antibodies against Pseudomonas aeruginosa in patients with cystic fibrosis – clinical application

2018 ◽  
Author(s):  
Guergana Petrova ◽  
Penka Perenovska ◽  
Spaska Lesichkova ◽  
Snezhina Lazova ◽  
Dimitrinka Miteva ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Clinical Application

scholarly journals 77* Impact of reducing routine sensitivity testing in chronic Pseudomonas aeruginosa infections in cystic fibrosis

2007 ◽  
Vol 6 ◽  
pp. S19
Author(s):  
C. Etherington ◽  
M. Hall ◽  
S. Conway ◽  
D. Peckham ◽  
M. Denton
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Sensitivity Testing

scholarly journals Targeted Antibiotic Prophylaxis for Lung Transplantation in Cystic Fibrosis Patients Colonised withPseudomonas aeruginosaUsing Multiple Combination Bactericidal Testing

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Helmy Haja Mydin ◽  
Paul A. Corris ◽  
Audrey Nicholson ◽  
John D. Perry ◽  
Gerard Meachery ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Lung Transplantation ◽  
Antibiotic Prophylaxis ◽  
Pleural Fluid ◽  
Conventional Group ◽  
Sensitivity Testing ◽  
Resistance Method ◽  
Clinical Records

Early infection is a recognised complication after lung transplantation in patients with cystic fibrosis (CF). Our centre uses multiple combination bactericidal testing (MCBT) when determining appropriate peritransplant prophylactic regimens. To evaluate our strategy, we compared the incidence of posttransplant infection in patients whose peritransplant antimicrobial regimens were determined using MCBT versus standard sensitivity testing. Patients with CF who were infected withPseudomonas aeruginosaand underwent lung transplantations between 2000 and 2010 were included. Data was collected from clinical records and our microbiology database. Microorganisms cultured were mapped against antibiotic resistance, method of sensitivity testing, and antibiotics administered peritransplant. 129 patients were identified (mean age 28, male : female, 63 : 66). Fifty patients (38.8%) had antibiotics determined by MCBT. Two patients in the MCBT group developed septicaemia, 13 in the conventional group (P≤0.05, 2-tailed Fisher's test). Sepsis was attributable toP. aeruginosain one patient from the MCBT group and seven patients in the conventional group (P=0.15).P. aeruginosawas recovered from the posttransplant pleural fluid of one patient who received MCBT-guided prophylaxis, six patients in the conventional group (P=0.25). Patients given antibiotics based on MCBT had significantly lower rates of septicaemia and lower rates of empyema.

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