scholarly journals Crystal structure of the human dual specificity phosphatase 1 catalytic domain

2017 ◽  
Vol 27 (2) ◽  
pp. 561-567 ◽  
Author(s):  
Rajesh Gumpena ◽  
George T. Lountos ◽  
Sreejith Raran-Kurussi ◽  
Joseph E. Tropea ◽  
Scott Cherry ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Catalytic Domain ◽  
Dual Specificity Phosphatase ◽  
Dual Specificity

High-resolution crystal structure of the catalytic domain of human dual-specificity phosphatase 26

2013 ◽  
Vol 69 (6) ◽  
pp. 1160-1170 ◽  
Author(s):  
Eun-Young Won ◽  
Yong Xie ◽  
Chie Takemoto ◽  
Lirong Chen ◽  
Zhi-Jie Liu ◽  
...  
Keyword(s):  
Crystal Structure ◽  
High Resolution ◽  
Catalytic Domain ◽  
Dual Specificity Phosphatase ◽  
Dual Specificity

scholarly journals Structural analysis of human dual-specificity phosphatase 22 complexed with a phosphotyrosine-like substrate

2015 ◽  
Vol 71 (2) ◽  
pp. 199-205 ◽  
Author(s):  
George T. Lountos ◽  
Scott Cherry ◽  
Joseph E. Tropea ◽  
David S. Waugh
Keyword(s):  
Crystal Structure ◽  
Structural Analysis ◽  
Phosphatase Activity ◽  
Small Molecule ◽  
Molecular Basis ◽  
Protein Tyrosine Phosphatases ◽  
Simple Method ◽  
Dual Specificity Phosphatase ◽  
Dual Specificity ◽  
Nitrophenyl Phosphate

4-Nitrophenyl phosphate (p-nitrophenyl phosphate, pNPP) is widely used as a small molecule phosphotyrosine-like substrate in activity assays for protein tyrosine phosphatases. It is a colorless substrate that upon hydrolysis is converted to a yellow 4-nitrophenolate ion that can be monitored by absorbance at 405 nm. Therefore, the pNPP assay has been widely adopted as a quick and simple method to assess phosphatase activity and is also commonly used in assays to screen for inhibitors. Here, the first crystal structure is presented of a dual-specificity phosphatase, human dual-specificity phosphatase 22 (DUSP22), in complex with pNPP. The structure illuminates the molecular basis for substrate binding and may also facilitate the structure-assisted development of DUSP22 inhibitors.

scholarly journals Structure of human dual-specificity phosphatase 7, a potential cancer drug target

2015 ◽  
Vol 71 (6) ◽  
pp. 650-656 ◽  
Author(s):  
George T. Lountos ◽  
Brian P. Austin ◽  
Joseph E. Tropea ◽  
David S. Waugh
Keyword(s):  
Catalytic Domain ◽  
Three Dimensional ◽  
Mitogen Activated Protein Kinase ◽  
Cancer Drug ◽  
Dual Specificity Phosphatase ◽  
Dual Specificity ◽  
Starting Point ◽  
Mitogen Activated Protein ◽  
Dual Specificity Phosphatases

Human dual-specificity phosphatase 7 (DUSP7/Pyst2) is a 320-residue protein that belongs to the mitogen-activated protein kinase phosphatase (MKP) subfamily of dual-specificity phosphatases. Although its precise biological function is still not fully understood, previous reports have demonstrated that DUSP7 is overexpressed in myeloid leukemia and other malignancies. Therefore, there is interest in developing DUSP7 inhibitors as potential therapeutic agents, especially for cancer. Here, the purification, crystallization and structure determination of the catalytic domain of DUSP7 (Ser141–Ser289/C232S) at 1.67 Å resolution are reported. The structure described here provides a starting point for structure-assisted inhibitor-design efforts and adds to the growing knowledge base of three-dimensional structures of the dual-specificity phosphatase family.

Crystal structure of human dual specificity phosphatase, JNK stimulatory phosphatase-1, at 1.5 Å resolution

2006 ◽  
Vol 66 (2) ◽  
pp. 272-278 ◽  
Author(s):  
Takehiro Yokota ◽  
Yukinori Nara ◽  
Akiko Kashima ◽  
Keiko Matsubara ◽  
Satoru Misawa ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Dual Specificity Phosphatase ◽  
Dual Specificity

Crystal structure of the catalytic domain of human VHY, a dual-specificity protein phosphatase

2005 ◽  
Vol 61 (3) ◽  
pp. 694-697 ◽  
Author(s):  
Tae-Sung Yoon ◽  
Dae Gwin Jeong ◽  
Jae Hoon Kim ◽  
Yoon Hea Cho ◽  
Jeong Hee Son ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Protein Phosphatase ◽  
Catalytic Domain ◽  
Dual Specificity ◽  
Dual Specificity Protein Phosphatase ◽  
Specificity Protein

Crystal structure of the catalytic domain of human DUSP5, a dual specificity MAP kinase protein phosphatase

2006 ◽  
Vol 66 (1) ◽  
pp. 253-258 ◽  
Author(s):  
Dae Gwin Jeong ◽  
Yoon Hea Cho ◽  
Tae-Sung Yoon ◽  
Jae Hoon Kim ◽  
Seong Eon Ryu ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Map Kinase ◽  
Protein Phosphatase ◽  
Catalytic Domain ◽  
Dual Specificity
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