Tissue clearing techniques for three‐dimensional optical imaging of intact human prostate and correlations with multi‐parametric MRI

The Prostate ◽  
2021 ◽  
Author(s):  
Stefano Cipollari ◽  
Neema Jamshidi ◽  
Liutao Du ◽  
Kyunghyun Sung ◽  
Danshan Huang ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Three Dimensional ◽  
Human Prostate ◽  
Tissue Clearing

scholarly journals Tissue clearing techniques for three‐dimensional optical imaging of intact human prostate and correlations with multi‐parametric MRI

The Prostate ◽  
2021 ◽  
Author(s):  
Stefano Cipollari ◽  
Neema Jamshidi ◽  
Liutao Du ◽  
Kyunghyun Sung ◽  
Danshan Huang ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Three Dimensional ◽  
Human Prostate ◽  
Tissue Clearing

scholarly journals EZ Clear for simple, rapid, and robust mouse whole organ clearing

2022 ◽  
Author(s):  
Chih-Wei Hsu ◽  
Juan Cerda ◽  
Jason M Kirk ◽  
Williamson D. Turner ◽  
Tara L. Rasmussen ◽  
...  
Keyword(s):  
Biomedical Research ◽  
Room Temperature ◽  
Adult Mouse ◽  
Dimensional Space ◽  
Three Dimensional ◽  
Fluorescence Signal ◽  
Tissue Architecture ◽  
Tissue Clearing ◽  
Clearing Method ◽  
Three Dimensional Space

Tissue clearing for whole organ cell profiling has revolutionized biology and imaging for exploration of organs in three-dimensional space without compromising tissue architecture. But complicated, laborious procedures, or expensive equipment, as well as the use of hazardous, organic solvents prevents the widespread adoption of these methods. Here we report a simple and rapid tissue clearing method, EZ Clear, that can clear whole adult mouse organs in 48 hours in just three simple steps. Samples stay at room temperature and remain hydrated throughout the clearing process, preserving endogenous and synthetic fluorescence, without altering sample size. After wholemount clearing and imaging, EZ Cleared samples can be further processed for downstream embedding and cryosectioning followed by standard histology or immunostaining, without loss of endogenous or synthetic fluorescence signal. Overall, the simplicity, speed, and flexibility of EZ Clear make it easy to adopt and apply to diverse approaches in biomedical research.

Three-dimensional nanoscale subsurface optical imaging of silicon circuits

2007 ◽  
Vol 90 (13) ◽  
pp. 131101 ◽  
Author(s):  
E. Ramsay ◽  
K. A. Serrels ◽  
M. J. Thomson ◽  
A. J. Waddie ◽  
M. R. Taghizadeh ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Three Dimensional

scholarly journals Three-Dimensional Optical Imaging for Pectus Excavatum Assessment

2019 ◽  
Vol 108 (4) ◽  
pp. 1065-1071 ◽  
Author(s):  
Jordan S. Taylor ◽  
Sriraman Madhavan ◽  
Dalia Szafer ◽  
Allison Pei ◽  
Rajashree Koppolu ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Pectus Excavatum ◽  
Three Dimensional

scholarly journals Three-dimensional fuse deposition modeling of tissue-simulating phantom for biomedical optical imaging

2015 ◽  
Vol 20 (12) ◽  
pp. 121311 ◽  
Author(s):  
Erbao Dong ◽  
Zuhua Zhao ◽  
Minjie Wang ◽  
Yanjun Xie ◽  
Shidi Li ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Three Dimensional ◽  
Fuse Deposition Modeling ◽  
Biomedical Optical Imaging ◽  
Deposition Modeling

scholarly journals Three-dimensional imaging of KNDy neurons in the mammalian brain using optical tissue clearing and multiple-label immunocytochemistry

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Aleisha M. Moore ◽  
Kathryn A. Lucas ◽  
Robert L. Goodman ◽  
Lique M. Coolen ◽  
Michael N. Lehman
Keyword(s):  
Three Dimensional ◽  
Mammalian Brain ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Kndy Neurons

Tissue clearing‐based method for unobstructed three‐dimensional imaging of mouse penis with subcellular resolution

2020 ◽  
Vol 13 (7) ◽  
Author(s):  
Paweł Matryba ◽  
Artur Wolny ◽  
Monika Pawłowska ◽  
Anna Sosnowska ◽  
Zuzanna Rydzyńska ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Three Dimensional Imaging ◽  
Tissue Clearing ◽  
Subcellular Resolution
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