The carboxyl terminal domain ofEscherichia coli DNA topoisomerase I confers higher affinity to DNA

1989 ◽  
Vol 6 (3) ◽  
pp. 249-258 ◽  
Author(s):  
Rita K. Beran-Steed ◽  
Yuk-Ching Tse-Dinh
Keyword(s):  
Topoisomerase I ◽  
Ofescherichia Coli ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase ◽  
Terminal Domain ◽  
Carboxyl Terminal Domain ◽  
Carboxyl Terminal

scholarly journals Identification of an N-terminal domain of eukaryotic DNA topoisomerase I dispensable for catalytic activity but essential for in vivo function.

1992 ◽  
Vol 267 (18) ◽  
pp. 12408-12411
Author(s):  
J Alsner ◽  
J.Q. Svejstrup ◽  
E Kjeldsen ◽  
B.S. Sørensen ◽  
O Westergaard
Keyword(s):  
Catalytic Activity ◽  
Topoisomerase I ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase ◽  
Terminal Domain ◽  
Eukaryotic Dna

scholarly journals Carboxyl terminal domain basic amino acids of mycobacterial topoisomerase I bind DNA to promote strand passage

2013 ◽  
Vol 41 (15) ◽  
pp. 7462-7471 ◽  
Author(s):  
Wareed Ahmed ◽  
Anuradha Gopal Bhat ◽  
Majety Naga Leelaram ◽  
Shruti Menon ◽  
Valakunja Nagaraja
Keyword(s):  
Amino Acids ◽  
Topoisomerase I ◽  
Basic Amino Acids ◽  
Terminal Domain ◽  
Carboxyl Terminal Domain ◽  
Carboxyl Terminal ◽  
Strand Passage

scholarly journals Targeting to Transcriptionally Active Loci by the Hydrophilic N-Terminal Domain of Drosophila DNA Topoisomerase I

1998 ◽  
Vol 18 (7) ◽  
pp. 4358-4367 ◽  
Author(s):  
Wen-Ling Shaiu ◽  
Tao-shih Hsieh
Keyword(s):  
Heat Shock ◽  
Fusion Protein ◽  
Fusion Proteins ◽  
Topoisomerase I ◽  
Polytene Chromosomes ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase ◽  
Rna Pol Ii ◽  
Pol Ii ◽  
Terminal Domain

ABSTRACT DNA topoisomerase I (topo I) from Drosophila melanogaster contains a nonconserved, hydrophilic N-terminal domain of about 430 residues upstream of the conserved core domains. Deletion of this N terminus did not affect the catalytic activity of topo I, while further removal of sequences into the conserved regions inactivated its enzymatic activity. We have investigated the cellular function of the Drosophila topo I N-terminal domain withtop1-lacZ transgenes. There was at least one putative nuclear localization signal within the first 315 residues of the N-terminal domain that allows efficient import of the large chimeric proteins into Drosophila nuclei. The top1-lacZfusion proteins colocalized with RNA polymerase II (pol II) at developmental puffs on the polytene chromosomes. Either topo I or thetop1-lacZ fusion protein was colocalized with RNA pol II in some but not all of the nonpuff, interband loci. However, the fusion proteins as well as RNA pol II were recruited to heat shock puffs during heat treatment, and they returned to the developmental puffs after recovery from heat shock. By immunoprecipitation, we showed that two of the largest subunits of RNA pol II coprecipitated with the N-terminal 315-residue fusion protein by using antibodies against β-galactosidase. These data suggest that the topo I fusion protein can be localized to the transcriptional complex on chromatin and that the N-terminal 315 residues were sufficient to respond to cellular processes, especially during the reprogramming of gene expression.

scholarly journals Interaction between the N-terminal domain of human DNA topoisomerase I and the arginine-serine domain of its substrate determines phosphorylation of SF2/ASF splicing factor

1998 ◽  
Vol 26 (12) ◽  
pp. 2955-2962 ◽  
Author(s):  
E. Labourier ◽  
F. Rossi ◽  
I.-e. Gallouzi ◽  
E. Allemand ◽  
G. Divita ◽  
...  
Keyword(s):  
Topoisomerase I ◽  
Splicing Factor ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase ◽  
Terminal Domain ◽  
Human Dna ◽  
Human Dna Topoisomerase I

scholarly journals The C-terminal domain but not the tyrosine 723 of human DNA topoisomerase I active site contributes to kinase activity

1998 ◽  
Vol 26 (12) ◽  
pp. 2963-2970 ◽  
Author(s):  
F. Rossi ◽  
E. Labourier ◽  
I.-e. Gallouzi ◽  
J. Derancourt ◽  
E. Allemand ◽  
...  
Keyword(s):  
Active Site ◽  
Topoisomerase I ◽  
Kinase Activity ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase ◽  
Terminal Domain ◽  
Human Dna ◽  
Human Dna Topoisomerase I

scholarly journals cDNA cloning of human DNA topoisomerase I: catalytic activity of a 67.7-kDa carboxyl-terminal fragment.

1988 ◽  
Vol 85 (8) ◽  
pp. 2543-2547 ◽  
Author(s):  
P. D'Arpa ◽  
P. S. Machlin ◽  
H. Ratrie ◽  
N. F. Rothfield ◽  
D. W. Cleveland ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Cdna Cloning ◽  
Topoisomerase I ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase ◽  
Human Dna ◽  
Terminal Fragment ◽  
Carboxyl Terminal ◽  
Human Dna Topoisomerase I

scholarly journals Viability ofEscherichia coli topAMutants Lacking DNA Topoisomerase I

2004 ◽  
Vol 280 (1) ◽  
pp. 355-360 ◽  
Author(s):  
Vera A. Stupina ◽  
James C. Wang
Keyword(s):  
Topoisomerase I ◽  
Ofescherichia Coli ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase
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