scholarly journals Paralogous Cyp51s mediate the differential sensitivity ofFusarium oxysporumto sterol demethylation inhibitors

2018 ◽  
Vol 75 (2) ◽  
pp. 396-404 ◽  
Author(s):  
Bangxian Zheng ◽  
Leiyan Yan ◽  
Wenxing Liang ◽  
Qianqian Yang
Keyword(s):  
Differential Sensitivity ◽  
Sterol Demethylation Inhibitors

Paralogous cyp51 genes in Fusarium graminearum mediate differential sensitivity to sterol demethylation inhibitors

2011 ◽  
Vol 48 (2) ◽  
pp. 113-123 ◽  
Author(s):  
Xin Liu ◽  
Fangwei Yu ◽  
Guido Schnabel ◽  
Jianbing Wu ◽  
Zhengyi Wang ◽  
...  
Keyword(s):  
Fusarium Graminearum ◽  
Differential Sensitivity ◽  
Sterol Demethylation Inhibitors

scholarly journals Paralogous CYP51 Genes of Colletotrichum spp. Mediate Differential Sensitivity to Sterol Demethylation Inhibitors

2020 ◽  
Vol 110 (3) ◽  
pp. 615-625 ◽  
Author(s):  
Shuning Chen ◽  
Mengjun Hu ◽  
Guido Schnabel ◽  
Daibin Yang ◽  
Xiaojing Yan ◽  
...  
Keyword(s):  
Mycelial Growth ◽  
Synergistic Effects ◽  
Differential Sensitivity ◽  
Deletion Mutants ◽  
Yeast Saccharomyces Cerevisiae ◽  
Dmi Fungicides ◽  
Sterol Demethylation Inhibitors ◽  
Increased Sensitivity ◽  
Colletotrichum Spp ◽  
Species Specific

Colletotrichum spp. isolates contain two paralogous CYP51 genes that encode sterol 14-demethylase enzymes; however, their role in sensitivity to demethylation inhibitor (DMI) fungicides is yet to be determined. In this study, each of the two genes from Colletotrichum fioriniae and C. nymphaeae was able to rescue the function of CYP51 in the yeast Saccharomyces cerevisiae, demonstrating their independent function. Deletion of CYP51A led to increased sensitivity to propiconazole, diniconazole, prothioconazole, cyproconazole, epoxiconazole, flutriafol, prochloraz, and difenoconazole in C. fioriniae, and to the same fungicides and tebuconazole in C. nymphaeae, with the exception of prochloraz. Deletion of CYP51B in C. fioriniae and CYP51B in C. nymphaeae made mutants increasingly sensitive to five of nine DMI fungicides tested. The results suggest species-specific, differential binding of DMI fungicides onto the two CYP51 enzymes. Pairing DMIs with different effects on CYP51A and -B deletion mutants resulted in synergistic effects, as determined in mycelial growth inhibition experiments. Deletion mutants showed no fitness penalty in terms of mycelial growth, sporulation, and virulence. Our study elucidates the effect of CYP51A and CYP51B of Colletotrichum spp. on DMI sensitivity, suggesting that using a mixture of DMIs may improve the efficacy for anthracnose management.

Ultrastructural Changes in Three Different Mammalian Cells Following Ultraviolet Laser Irradiation

1972 ◽  
Vol 30 ◽  
pp. 350-351
Author(s):  
K. Shankar Narayan ◽  
Kailash C. Gupta ◽  
Tohru Okigaki
Keyword(s):  
Ultraviolet Light ◽  
Mammalian Cells ◽  
Biological Effects ◽  
Survival Rates ◽  
Chinese Hamster ◽  
Cell Types ◽  
Differential Sensitivity ◽  
Ultrastructural Changes ◽  
Ultraviolet Laser

The biological effects of short-wave ultraviolet light has generally been described in terms of changes in cell growth or survival rates and production of chromosomal aberrations. Ultrastructural changes following exposure of cells to ultraviolet light, particularly at 265 nm, have not been reported.We have developed a means of irradiating populations of cells grown in vitro to a monochromatic ultraviolet laser beam at a wavelength of 265 nm based on the method of Johnson. The cell types studies were: i) WI-38, a human diploid fibroblast; ii) CMP, a human adenocarcinoma cell line; and iii) Don C-II, a Chinese hamster fibroblast cell strain. The cells were exposed either in situ or in suspension to the ultraviolet laser (UVL) beam. Irradiated cell populations were studied either "immediately" or following growth for 1-8 days after irradiation.Differential sensitivity, as measured by survival rates were observed in the three cell types studied. Pattern of ultrastructural changes were also different in the three cell types.

Differential sensitivity for temporal intervals with auditory stimulation

1960 ◽  
Author(s):  
A. M. Small ◽  
R. A. Campbell
Keyword(s):  
Auditory Stimulation ◽  
Differential Sensitivity ◽  
Temporal Intervals

Differential sensitivity to beta-cell secretagogues in "early," type I diabetes mellitus

Diabetes ◽  
1984 ◽  
Vol 33 (6) ◽  
pp. 516-521 ◽  
Author(s):  
O. P. Ganda ◽  
S. Srikanta ◽  
S. J. Brink ◽  
M. A. Morris ◽  
R. E. Gleason ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Beta Cell ◽  
Type I Diabetes ◽  
Type I Diabetes Mellitus ◽  
Differential Sensitivity ◽  
Type I ◽  
Early Type
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