Cuticle thickening associated with fenitrothion and imidacloprid resistance and influence of voltage‐gated sodium channel mutations on pyrethroid resistance in the tropical bed bug, Cimex hemipterus

2021 ◽  
Author(s):  
Li‐Shen Soh ◽  
G. Veera Singham
Keyword(s):  
Sodium Channel ◽  
Pyrethroid Resistance ◽  
Voltage Gated Sodium Channel ◽  
Bed Bug ◽  
Voltage Gated ◽  
Cimex Hemipterus

scholarly journals A mutation in the voltage-gated sodium channel gene associated with pyrethroid resistance in Latin American Aedes aegypti

2007 ◽  
Vol 16 (6) ◽  
pp. 785-798 ◽  
Author(s):  
K. Saavedra-Rodriguez ◽  
L. Urdaneta-Marquez ◽  
S. Rajatileka ◽  
M. Moulton ◽  
A. E. Flores ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Latin American ◽  
Pyrethroid Resistance ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Channel Gene ◽  
Sodium Channel Gene

Pyrethroid resistance in Sitophilus zeamais is associated with a mutation (T929I) in the voltage-gated sodium channel

2011 ◽  
Vol 20 (4) ◽  
pp. 437-445 ◽  
Author(s):  
Rúbia A. Araújo ◽  
Martin S. Williamson ◽  
Christopher Bass ◽  
Linda M. Field ◽  
Ian R. Duce
Keyword(s):  
Sodium Channel ◽  
Pyrethroid Resistance ◽  
Sitophilus Zeamais ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated

scholarly journals Detection of Polymorphism on Voltage-gated Sodium Channel Gene of Indonesian Aedes aegypti Associated with Resistance to Pyrethroids

2018 ◽  
Vol 10 (3) ◽  
pp. 250-5 ◽  
Author(s):  
Budi Mulyaningsih ◽  
Sitti Rahmah Umniyati ◽  
Tri Baskoro Tunggul Satoto ◽  
Ernaningsih Ernaningsih ◽  
Dwi Aris Agung Nugrahaningsih
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Sodium Channel ◽  
Pyrethroid Resistance ◽  
Single Amino Acid ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Channel Gene ◽  
Sodium Channel Gene ◽  
Yogyakarta City

BACKGROUND: Aedes aegypti is a vector of several pathogens including dengue virus. Vector control is an effective way to break the transmission but unfortunately constant use of insecticides ultimately causes vector resistance. Pyrethroids have been used for about 15 years to combat Ae. aegypti in Yogyakarta Province, Indonesia. Single amino acid substitutions in the voltagegated sodium channel associated with pyrethroid resistance constitute one of the main causative factors of knockdown resistance (kdr). The aim of this study is to detect resistant status to cypermetrine and polymorphism on the voltagegated sodium channel gene of Ae. aegypti from 2 dengue endemic areas in Yogyakarta Province (Yogyakarta city and Sleman district).METHODS: Pyrethroid resistance in Ae. aegypti mosquitoes was detected by using CDC Bottle Bioassay. To detect the polymorphism on the voltage-gated sodium channel gene of Ae. aegypti analyses were conducted by using PCR and direct DNA squencing with primers AaSCF1 and AaSCR4 for S989P, I1011M (or V), L1014F sites, and AaSCF7 and AaSCR7 for the F1534C site.RESULTS: According to bioassay, the results for Ae. aegypti from Yogyakarta city (93% mortality) and Sleman district (88% mortality) suggest the possibility of resistance to cypermethrin. We observed polymorphism on voltagegated sodium channel gene on site F1534C (heterozygous).CONCLUSION: The findings provide early evidence that the use of cypermethrin (pyrethroids) in Yogyakarta city and Sleman district, Yogyakarta Province, Indonesia is reducing its effectiveness to control Ae. aegypti. Recommendations include additional tests for confirmation.KEYWORDS: Aedes aegypti, dengue virus, cypermethrin, Bioassay, Yogyakarta

scholarly journals Identification of mutations associated with pyrethroid resistance in the voltage-gated sodium channel of the tomato leaf miner (Tuta absoluta)

2012 ◽  
Vol 42 (7) ◽  
pp. 506-513 ◽  
Author(s):  
Khalid Haddi ◽  
Madeleine Berger ◽  
Pablo Bielza ◽  
Dina Cifuentes ◽  
Linda M. Field ◽  
...  
Keyword(s):  
Sodium Channel ◽  
Pyrethroid Resistance ◽  
Tomato Leaf ◽  
Tuta Absoluta ◽  
Leaf Miner ◽  
Voltage Gated Sodium Channel ◽  
Voltage Gated ◽  
Tomato Leaf Miner

Novel CONCOMITANT mutations L932F and I936V in the Voltage-Gated Sodium Channel and Its Association With Pyrethroid Resistance in Culex quinquefasciatus (Diptera: Culicidae)

2020 ◽  
Author(s):  
Masaaki Sugiura ◽  
Fumiko Kimoto ◽  
Kentaro Itokawa ◽  
Shinji Kasai
Keyword(s):  
Cytochrome P450 ◽  
Sodium Channel ◽  
Culex Quinquefasciatus ◽  
Pyrethroid Resistance ◽  
Cross Resistance ◽  
Kdr Mutation ◽  
Voltage Gated Sodium Channel ◽  
Important Species ◽  
Voltage Gated ◽  
Brazilian Strain

Abstract Highly residual pyrethroids such as permethrin have been used for controlling mosquitoes that transmit infectious diseases. However, the selective pressure from such insecticides may result in cross-resistance against other pyrethroids used for household insecticides. In this study, we investigated the susceptibility of Culex quinquefasciatus Say collected from Brazil and Myanmar to permethrin in addition to four types of household pyrethroids. Both strains exhibited high resistance against all pyrethroids tested, indicating cross-resistance. Furthermore, we detected the knockdown resistance (kdr) mutations L932F+I936V in the voltage-gated sodium channel gene (VGSC) in the Brazilian strain. Notably, the L932F+I936V haplotype has previously been observed in in silico data, but it should be detected not directly from living insects. In comparison, a common kdr mutation, L1014F, was detected from the Myanmar strain. Although L1014F was also detected from the Brazilian strain, the allele frequency was too low to affect resistance. Both strains harbored the resistance-associated haplotypes of the cytochrome P450 gene, CYP9M10. The Brazilian strain demonstrated comparable resistance against pyrethroids as that of the Myanmar strain even when a cytochrome P450 inhibitor, piperonyl butoxide was added to the bioassay. Our results suggested that the L932F+I936V mutations confer the Brazilian strain of Cx. Quiquefasciatus with resistance at a comparable level to that conferred by the well-recognized kdr mutation L1014F in the Myanmar strain. The identification of unexplored mutations may improve the diagnosis and understanding of resistance of this medically important species.

scholarly journals Loss of pyrethroid resistance in newly established laboratory colonies of Aedes aegypti

2019 ◽  
Author(s):  
Farah Z. Vera-Maloof ◽  
Karla Saavedra-Rodriguez ◽  
Rosa P. Penilla-Navarro ◽  
Americo D. Rodriguez-Ramirez ◽  
Felipe Dzul ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Sodium Channel ◽  
Pyrethroid Resistance ◽  
Natural Populations ◽  
Resistance Management ◽  
Target Site ◽  
Voltage Gated Sodium Channel ◽  
Pyrethroid Insecticides ◽  
Voltage Gated ◽  
Frequent Use

AbstractBackgroundResistance to pyrethroid insecticides in Aedes aegypti has become widespread after almost two decades of their frequent use to reduce arbovirus transmission. Despite this, use of pyrethroids continues because they are relatively inexpensive and because of their low human toxicity. Resistance management has been proposed as a means to retain the use of pyrethroids in natural populations. A key component of resistance management assumes that there is a negative fitness associated with resistance alleles so that when insecticides are removed, resistance alleles will decline in frequency. At least three studies in Ae. aegypti have demonstrated a decrease in pyrethroid resistance once the insecticide is removed.Methods/Principal FindingsThe present study aims to evaluate variation in the loss of pyrethroid resistance among newly established laboratory populations of Ae. aegypti from Mexico. Eight field collections were maintained for up to eight generations and we recorded changes in the frequencies of mutations at the V1,016I locus and at the F1,534C locus in the voltage gated sodium channel (VGSC) gene. I1,016 and C1,534 confer resistance. We also examined resistance ratios (RR) with type 1 and 2 pyrethroids.Conclusions/SignificanceWe demonstrate that, in general, the frequency of the Ae. aegypti pyrethroid resistance alleles I1,016 and C1,534 decline when they are freed from pyrethroid pressure in the laboratory. However, the pattern of decline is strain dependent. In agreement with earlier studies, RR was positively correlated with I1,016 resistant allele frequencies and showed significant protection against permethrin, and deltamethrin whereas F1534C showed protection against permethrin but not against deltamethrin.Author SummaryThe author is interested in the evolution of genes that confer resistance to insecticides, especially when this evolution affects binding of insecticides to their target site. The Voltage Gated Sodium Channel gene represents an excellent opportunity to understand how mutations at the target site(s) affect the evolution of resistance in many different pest insect species including Aedes aegypti, the primary vector of Dengue Virus, Yellow Fever, Zika and Chikungunya arboviruses.

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