Isolation of ginsenoside Rb1 fromKalopanax pictus by eastern blotting using anti-ginsenoside Rb1 monoclonal antibody

2005 ◽  
Vol 19 (3) ◽  
pp. 255-258 ◽  
Author(s):  
Hiroyuki Tanaka ◽  
Noriko Fukuda ◽  
Shoji Yahara ◽  
Susumu Isoda ◽  
Chun-Su Yuan ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Ginsenoside Rb1 ◽  
Eastern Blotting

scholarly journals Development of Eastern Blotting Technique for Analysis of Baicalin Using Anti-Baicalin Monoclonal Antibody

Antibodies ◽  
2012 ◽  
Vol 1 (3) ◽  
pp. 284-293 ◽  
Author(s):  
Osamu Morinaga ◽  
Ryo Mukae ◽  
Takuhiro Uto ◽  
Yothawathorn Pariyawongsakul ◽  
Waraporn Putalun ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Eastern Blotting ◽  
Blotting Technique

scholarly journals Fingerprinting of Natural Product by Eastern Blotting Using Monoclonal Antibodies

2012 ◽  
Vol 2012 ◽  
pp. 1-7
Author(s):  
Hiroyuki Tanaka ◽  
Waraporn Putalun ◽  
Yukihiro Shoyama
Keyword(s):  
Monoclonal Antibodies ◽  
Natural Product ◽  
Cross Reactivity ◽  
Blue Color ◽  
Ginsenoside Rb1 ◽  
Double Staining ◽  
Eastern Blotting ◽  
The Central Nervous System ◽  
Rf Values ◽  
Solasodine Glycosides

We succeeded in developing the fingerprint of natural product by eastern blotting using monoclonal antibodies. After developing and separating them on a TLC plate, solasodine glycosides are oxidized by NaIO4 and reacted with a protein to give conjugates which are recognized with anti-solamargine monoclonal antibody (MAb). Anti-solamargine MAb having wide cross-reactivity can stain and detect all solasodine glycosides by fingerprint. Different sensitivity between solamargine and solasonine was observed. The detection limit was 1.6 ng of solasonine. The hydrolysed products of solamargine were determined by fingerprint of eastern blotting compared to their Rf values depending on the sugar number. Fingerprint by eastern blotting using anti-ginsenoside Rb1 MAb distinguished the formula containing ginseng prescribed in traditional Chinese medicine. By double-staining of ginsenosides it is possible to suggest that the staining color shows the pharmacological activity, such as the purple bands indicate ginsenosides having stimulation activity, and the blue color indicated compound like ginsenosides possessed the depression affect for the central nervous system (CNS), respectively.

A web-like network of type vi collagen in human skin is revealed by immuno-electron microscopy

1988 ◽  
Vol 46 ◽  
pp. 382-383
Author(s):  
Douglas R. Keene ◽  
Robert W. Glanville ◽  
Eva Engvall
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Human Skin ◽  
Basement Membranes ◽  
Primary Antibody ◽  
Fat Cells ◽  
Secondary Antibody ◽  
Type Vi Collagen ◽  
Dermal Matrix ◽  
Immuno Electron Microscopy

A mouse monoclonal antibody (5C6) prepared against human type VI collagen (1) has been used in this study to immunolocalize type VI collagen in human skin. The enbloc method used involves exposing whole tissue pieces to primary antibody and 5 nm gold conjugated secondary antibody before fixation, and has been described in detail elsewhere (2).Biopsies were taken from individuals ranging in age from neonate to 65 years old. By immuno-electron microscopy, type VI collagen is found to be distributed as a fine branching network closely associated with (but not attached to) banded collagen fibrils containing types I and III collagen (Fig. 1). It appears to enwrap fibers, to weave between individual fibrils within a fiber, and to span the distance separating fibers, creating a “web-like network” which entraps fibers within deep papillary and reticular dermal layers (Fig. 2). Relative to that in the dermal matrix, the concentration of type VI collagen is higher around endothelial basement membranes limiting the outer boundaries of nerves, capillaries, and fat cells (Fig. 3).

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