Development of an Eastern Blotting Technique for the Visual Detection of Aristolochic Acids in Aristolochia and Asarum Species by Using a Monoclonal Antibody Against Aristolochic Acids I and II

2013 ◽  
Vol 24 (6) ◽  
pp. 645-653 ◽  
Author(s):  
Xiao-Wei Li ◽  
Osamu Morinaga ◽  
Min Tian ◽  
Takuhiro Uto ◽  
Jie Yu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Visual Detection ◽  
Aristolochic Acids ◽  
Eastern Blotting ◽  
Blotting Technique

scholarly journals Development of Eastern Blotting Technique for Analysis of Baicalin Using Anti-Baicalin Monoclonal Antibody

Antibodies ◽  
2012 ◽  
Vol 1 (3) ◽  
pp. 284-293 ◽  
Author(s):  
Osamu Morinaga ◽  
Ryo Mukae ◽  
Takuhiro Uto ◽  
Yothawathorn Pariyawongsakul ◽  
Waraporn Putalun ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Eastern Blotting ◽  
Blotting Technique

scholarly journals Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Hiroyuki Tanaka ◽  
Osamu Morinaga ◽  
Takuhiro Uto ◽  
Shunsuke Fuji ◽  
Frederick Asare Aboagye ◽  
...  
Keyword(s):  
No Production ◽  
Laser Desorption Ionization ◽  
Double Staining ◽  
Immunoaffinity Column ◽  
New Techniques ◽  
Pvdf Membrane ◽  
Bioactive Natural Products ◽  
Eastern Blotting ◽  
Complete Identification ◽  
Blotting Technique

Matrix-assisted laser desorption/ionization (MALDI) tof mass spectrometry was used for the confirmation of hapten number in synthesized antigen. As application of MAb, the MAbs against ginsenosides and glycyrrhizin have been prepared resulting in the development of two new techniques that we named the eastern blotting method and the knockout extract preparation. In eastern blotting technique, glycosides like ginsenosides and glycyrrhizin separated by silica gel TLC were blotted to PVDF membrane that was treated with a NaIO4solution followed by BSA resulted in glycoside-BSA conjugate on a PVDF membrane. The blotted spots were stained by MAb. Double staining of eastern blotting for ginsenosides using antiginsenoside Rb1and Rg1MAbs promoted complete identification of ginsenosides inPanaxspecies. The immunoaffinity concentration of glycyrrhizin was determined by immunoaffinity column conjugated with antiglycyrrhizin MAb resulting in the glycyrrhizin-knockout extract, which was determined by the synergic effect with glycyrrhizin on NO production using the cell line.

scholarly journals Hybridomas for production of monoclonal antibodies to human erythropoietin

Blood ◽  
1984 ◽  
Vol 64 (2) ◽  
pp. 357-364 ◽  
Author(s):  
S Yanagawa ◽  
S Yokoyama ◽  
K Hirade ◽  
R Sasaki ◽  
H Chiba ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Growth Rate ◽  
Monoclonal Antibodies ◽  
Mechanism Of Action ◽  
Rapid Growth ◽  
Human Erythropoietin ◽  
Rapid Growth Rate ◽  
Blotting Technique ◽  
Purification Methods ◽  
High Production

Abstract Human urinary erythropoietin has been highly purified by a combination of conventional purification methods and immunoadsorbent columns packed with hybridoma-produced antibodies against contaminants that seemed difficult to separate from erythropoietin by the usual means. By using the partially purified erythropoietin as an antigen, three hybridoma clones have been obtained that secrete monoclonal antibodies against erythropoietin. One of the clones has been quite stable, with a rapid growth rate and high production of antibody. Western blotting technique with monoclonal antibodies revealed occurrence of two species of erythropoietin. The monoclonal antibody will be useful as a probe for the purification of erythropoietin and for further studies of the hormone and its mechanism of action.

Evaluation of a new eastern blotting technique for the analysis of ginsenoside Re in American ginseng berry pulp extracts

Fitoterapia ◽  
2010 ◽  
Vol 81 (4) ◽  
pp. 284-288 ◽  
Author(s):  
Osamu Morinaga ◽  
Takuhiro Uto ◽  
Chun-Su Yuan ◽  
Hiroyuki Tanaka ◽  
Yukihiro Shoyama
Keyword(s):  
American Ginseng ◽  
Ginsenoside Re ◽  
Eastern Blotting ◽  
Blotting Technique ◽  
Analysis Of Ginsenoside

scholarly journals Hybridomas for production of monoclonal antibodies to human erythropoietin

Blood ◽  
1984 ◽  
Vol 64 (2) ◽  
pp. 357-364 ◽  
Author(s):  
S Yanagawa ◽  
S Yokoyama ◽  
K Hirade ◽  
R Sasaki ◽  
H Chiba ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Growth Rate ◽  
Monoclonal Antibodies ◽  
Mechanism Of Action ◽  
Rapid Growth ◽  
Human Erythropoietin ◽  
Rapid Growth Rate ◽  
Blotting Technique ◽  
Purification Methods ◽  
High Production

Human urinary erythropoietin has been highly purified by a combination of conventional purification methods and immunoadsorbent columns packed with hybridoma-produced antibodies against contaminants that seemed difficult to separate from erythropoietin by the usual means. By using the partially purified erythropoietin as an antigen, three hybridoma clones have been obtained that secrete monoclonal antibodies against erythropoietin. One of the clones has been quite stable, with a rapid growth rate and high production of antibody. Western blotting technique with monoclonal antibodies revealed occurrence of two species of erythropoietin. The monoclonal antibody will be useful as a probe for the purification of erythropoietin and for further studies of the hormone and its mechanism of action.

scholarly journals Human Ia beta chains and the invariant chain share a common antigenic determinant.

1983 ◽  
Vol 158 (4) ◽  
pp. 1344-1349 ◽  
Author(s):  
O J Finn ◽  
C J Stackhouse ◽  
R S Metzgar
Keyword(s):  
Monoclonal Antibody ◽  
Antigenic Determinant ◽  
Light Chains ◽  
Invariant Chain ◽  
Two Dimensional ◽  
Ia Antigens ◽  
Sds Page ◽  
Blotting Technique ◽  
Specific Proteins ◽  
Beta 2

We have presented evidence that a mouse monoclonal antibody 1,227, which was previously shown to recognize a determinant on the light chains beta 1 and beta 2 of human Ia antigens, also recognizes a determinant on the invariant chain (1) associated with these molecules. Ia-specific proteins were resolved by two-dimensional (2D) PAGE and electrophoretically transferred onto nitrocellulose filters. The presence of a determinant shared between beta 1, beta 2 and I was established using "Western blotting" technique. In addition, we demonstrated that 1,227 can immunoprecipitate isolated beta 1, beta 2, and the invariant chain proteins following their separate elution from SDS-PAGE.

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