High-throughput analysis of peptides and proteins by laserspray ionization mass spectrometry

2010 ◽  
Vol 25 (1) ◽  
pp. 247-250 ◽  
Author(s):  
Alicia L. Richards ◽  
Darrell D. Marshall ◽  
Ellen D. Inutan ◽  
Charles N. McEwen ◽  
Sarah Trimpin
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Laserspray Ionization

Versatile Platform Employing Desorption Electrospray Ionization Mass Spectrometry for High-Throughput Analysis

2008 ◽  
Vol 80 (15) ◽  
pp. 6131-6136 ◽  
Author(s):  
Xiaoxiao Ma ◽  
Mengxia Zhao ◽  
Ziqing Lin ◽  
Sichun Zhang ◽  
Chengdui Yang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
High Throughput ◽  
Electrospray Ionization Mass Spectrometry ◽  
Desorption Electrospray Ionization ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
High Throughput Analysis ◽  
Throughput Analysis

High-Throughput Label-Free Biochemical Assays Using Infrared Matrix-Assisted Desorption Electrospray Ionization Mass Spectrometry

2021 ◽  
Vol 93 (17) ◽  
pp. 6792-6800
Author(s):  
Fan Pu ◽  
Andrew J. Radosevich ◽  
James W. Sawicki ◽  
David Chang-Yen ◽  
Nari N. Talaty ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
High Throughput ◽  
Electrospray Ionization Mass Spectrometry ◽  
Desorption Electrospray Ionization ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Label Free ◽  
Biochemical Assays

scholarly journals Development of a Novel Label-Free and High-Throughput Arginase-1 Assay Using Self-Assembled Monolayer Desorption Ionization Mass Spectrometry

2021 ◽  
pp. 247255522110006
Author(s):  
Michael D. Scholle ◽  
Zachary A. Gurard-Levin
Keyword(s):  
Mass Spectrometry ◽  
Drug Discovery ◽  
High Throughput ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Label Free ◽  
Desorption Ionization ◽  
Arginase 1 ◽  
Self Assembled ◽  
High Throughput Assay

Arginase-1, an enzyme that catalyzes the reaction of L-arginine to L-ornithine, is implicated in the tumor immune response and represents an interesting therapeutic target in immuno-oncology. Initiating arginase drug discovery efforts remains a challenge due to a lack of suitable high-throughput assay methodologies. This report describes the combination of self-assembled monolayers and matrix-assisted laser desorption ionization mass spectrometry to enable the first label-free and high-throughput assay for arginase activity. The assay was optimized for kinetically balanced conditions and miniaturized, while achieving a robust assay (Z-factor > 0.8) and a significant assay window [signal-to-background ratio > 20] relative to fluorescent approaches. To validate the assay, the inhibition of the reference compound nor-NOHA (Nω-hydroxy-nor-L-arginine) was evaluated, and the IC50 measured to be in line with reported results (IC50 = 180 nM). The assay was then used to complete a screen of 175,000 compounds, demonstrating the high-throughput capacity of the approach. The label-free format also eliminates opportunities for false-positive results due to interference from library compounds and optical readouts. The assay methodology described here enables new opportunities for drug discovery for arginase and, due to the assay flexibility, can be more broadly applicable for measuring other amino acid–metabolizing enzymes.

High-throughput quantification of sodium saccharin in foods by ambient flame ionization mass spectrometry

Talanta ◽  
2018 ◽  
Vol 182 ◽  
pp. 241-246 ◽  
Author(s):  
Zhongquan Li ◽  
Fang Zhang ◽  
Junbo Zhao ◽  
Xiaopan Liu ◽  
Xiuping Chen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Flame Ionization ◽  
Sodium Saccharin
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