scholarly journals Mass spectrometric identification of the N -monosubstituted N -hydroxylamino functionality in protonated analytes via ion/molecule reactions in tandem mass spectrometry

2015 ◽  
Vol 29 (8) ◽  
pp. 730-734 ◽  
Author(s):  
Huaming Sheng ◽  
Weijuan Tang ◽  
Ravikiran Yerabolu ◽  
John Y. Kong ◽  
Peggy E. Williams ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Mass Spectrometric ◽  
Tandem Mass ◽  
Ion Molecule Reactions ◽  
Spectrometric Identification ◽  
Mass Spectrometric Identification

Vitamin D analyses in veterinary feeds by gas chromatography-tandem mass spectrometry

2021 ◽  
pp. 146906672110002
Author(s):  
Andreas Lehner ◽  
Margaret Johnson ◽  
Alan Zimmerman ◽  
Justin Zyskowski ◽  
John Buchweitz
Keyword(s):  
Mass Spectrometry ◽  
Vitamin D ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Vitamin D3 ◽  
Mass Spectrometric ◽  
Chromatographic Behavior ◽  
Tandem Mass ◽  
Chromatography Tandem Mass Spectrometry

This report examines the feasibility of determination of Vitamin D3, D2 and their 25-hydroxy metabolites utilizing Gas Chromatography Tandem Mass Spectrometry (GC/MS/MS) as a potential alternative to popular Liquid Chromatography Tandem Mass Spectrometric (LC/MS/MS) methodologies. The GC/MS/MS approach was found to operate reasonably well despite long-standing concerns that gas-liquid chromatography of vitamin D compounds invoke thermal rearrangements owing to the relatively high inlet and capillary column temperatures used. The workup procedure involved incubation of feed samples with concentrated potassium hydroxide for overnight fat saponification, extraction of D Vitamins in n-hexane and reaction with N,O-bis(trimethylsilyl)trifluoroacetamide at 70 °C for 30 mins. In addition to parent compounds, small amounts of pyro-, isopyro-, and iso-vitamin D and isotachysterol3 variants were obtained from each Vitamin D-related compound upon extraction and GC/MS/MS analysis. Mass spectral and chromatographic behavior of these compounds are herein described and interpreted. Multiple Reaction Monitoring settings on GC/MS/MS included m/z 456→351 for Vitamin D3 and m/z 486→363 for Vitamin D2. Trimethylsilylation enabled single predominant peaks for Vitamins D3 and D2, and sample workup in the presence of deuterated Vitamin D analogs enabled accurate and precise sensitivity to 1 ppb (ng/g) in feeds. The method could be extended with reasonable accuracy to 25-hydroxy (25OH) compounds, but accuracies would be significantly improved by inclusion of respective 25OH-specific deuterated internal standards. The method was applied to 27 submissions of suspect dog foods of which 22% were discovered elevated and 44% were discovered to contain toxic levels of Vitamin D3. The described method was thus discovered to provide a suitable mass spectrometric approach for Vitamin D, proving itself here specifically of value in detection of ergocalciferol and cholecalciferol in animal feeds. The specificity and sensitivity of the tandem quadrupole approach can enable suitable applicability to serum determination if desired.

Tandem mass spectrometric identification of dextrose markers in dried-blood spots from infants receiving total parenteral nutrition

2010 ◽  
Vol 411 (21-22) ◽  
pp. 1806-1816 ◽  
Author(s):  
Donald H. Chace ◽  
Víctor R. De Jesús ◽  
Timothy H. Lim ◽  
W. Harry Hannon ◽  
Alan R. Spitzer
Keyword(s):  
Parenteral Nutrition ◽  
Total Parenteral Nutrition ◽  
Dried Blood Spots ◽  
Mass Spectrometric ◽  
Tandem Mass ◽  
Blood Spots ◽  
Spectrometric Identification ◽  
Mass Spectrometric Identification ◽  
Tandem Mass Spectrometric

Ion–molecule reactions of trimethylborate allow the mass spectrometric identification and counting of functional groups in protonated bifunctional oxygen-containing compounds and polyols

2007 ◽  
Vol 265 (2-3) ◽  
pp. 359-371 ◽  
Author(s):  
Jayalakshmi Somuramasami ◽  
Penggao Duan ◽  
Michael A. Watkins ◽  
Brian E. Winger ◽  
Hilkka I. Kenttamaa
Keyword(s):  
Functional Groups ◽  
Mass Spectrometric ◽  
Ion Molecule Reactions ◽  
Spectrometric Identification ◽  
Mass Spectrometric Identification
Sign in / Sign up

Export Citation Format

Share Document