scholarly journals Adenoviral Gene Delivery Can Reprogram Human Fibroblasts to Induced Pluripotent Stem Cells

Stem Cells ◽  
2009 ◽  
Vol 27 (11) ◽  
pp. 2667-2674 ◽  
Author(s):  
Wenbo Zhou ◽  
Curt R. Freed
Keyword(s):  
Stem Cells ◽  
Gene Delivery ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Fibroblasts ◽  
Adenoviral Gene Delivery ◽  
Induced Pluripotent

An insight into non-integrative gene delivery approaches to generate transgene-free induced pluripotent stem cells

Gene ◽  
2019 ◽  
Vol 686 ◽  
pp. 146-159 ◽  
Author(s):  
Krishna Kumar Haridhasapavalan ◽  
Manash P. Borgohain ◽  
Chandrima Dey ◽  
Bitan Saha ◽  
Gloria Narayan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Delivery ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent ◽  
Insight Into

scholarly journals Young at Heart: Pioneering Approaches to Model Nonischaemic Cardiomyopathy with Induced Pluripotent Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-15 ◽  
Author(s):  
Aoife Gowran ◽  
Marco Rasponi ◽  
Roberta Visone ◽  
Patrizia Nigro ◽  
Gianluca L. Perrucci ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Induced Pluripotent Stem Cells ◽  
Drug Screening ◽  
Pluripotent Stem Cells ◽  
Pluripotent Stem Cell ◽  
Human Fibroblasts ◽  
Induced Pluripotent Stem Cell ◽  
Induced Pluripotent

A mere 9 years have passed since the revolutionary report describing the derivation of induced pluripotent stem cells from human fibroblasts and the first in-patient translational use of cells obtained from these stem cells has already been achieved. From the perspectives of clinicians and researchers alike, the promise of induced pluripotent stem cells is alluring if somewhat beguiling. It is now evident that this technology is nascent and many areas for refinement have been identified and need to be considered before induced pluripotent stem cells can be routinely used to stratify, treat and cure patients, and to faithfully model diseases for drug screening purposes. This review specifically addresses the pioneering approaches to improve induced pluripotent stem cell based models of nonischaemic cardiomyopathy.

scholarly journals Enhanced Generation of Induced Pluripotent Stem Cells from a Subpopulation of Human Fibroblasts

PLoS ONE ◽  
2009 ◽  
Vol 4 (9) ◽  
pp. e7118 ◽  
Author(s):  
James A. Byrne ◽  
Ha Nam Nguyen ◽  
Renee A. Reijo Pera
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Fibroblasts ◽  
Induced Pluripotent

scholarly journals Derivation of human induced pluripotent stem cells through continued exposure of OCT4-induced plastic human fibroblasts to reprogramming media

2015 ◽  
Vol 15 (1) ◽  
pp. 240-242 ◽  
Author(s):  
Kyle R. Salci ◽  
Jung Bok Lee ◽  
Ryan R. Mitchell ◽  
Luca Orlando ◽  
Aline Fiebig-Comyn ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Fibroblasts ◽  
Induced Pluripotent

scholarly journals Reprogramming of Human Fibroblasts to Induced Pluripotent Stem Cells Under Xeno-Free Conditions

Stem Cells ◽  
2009 ◽  
pp. N/A-N/A ◽  
Author(s):  
Ignasi Rodríguez-Pizà ◽  
Yvonne Richaud-Patin ◽  
Rita Vassena ◽  
Federico González ◽  
María José Barrero ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Fibroblasts ◽  
Induced Pluripotent

scholarly journals Growth Factor-free Chondrogenic Differentiation from Induced Pluripotent Stem Cells using Minicircle Vectors

2018 ◽  
Author(s):  
Yeri Alice Rim ◽  
Yoojun Nam ◽  
Ji Hyeon Ju
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Growth Factor ◽  
Gene Delivery ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Viral Gene ◽  
Induced Pluripotent ◽  
Viral Gene Delivery

The human degenerative cartilage has low regenerative potential. Chondrocyte transplantation offers a promising strategy for cartilage treatment and regeneration. Currently chondrogenesis using human pluripotent stem cells are accomplished using human recombinant growth factors. Here, we differentiated human induced pluripotent stem cells (hiPSCs) into chondrocytes and cartilage pellet using minicircle vectors. Minicircles are used as a non-viral gene delivery system for gene therapy in various diseases. Non-viral gene delivery can produce growth factors without integrating into the host genome. Minicircle vectors containing bone morphogenetic protein 2 (BMP2) and transforming growth factor, beta 3 (TGFβ3) were successfully generated and delivered to hiPSC-derived outgrowth (OG) cells. Cell pellets generated using minicircle-transfected OG cells successfully differentiated into chondrogenic lineage. Chondrogenic pellets transfected with growth factor-encoding minicircles effectively recovered osteochondral defect in rat models. Taken together, this work shows the potential application of minicircles in cartilage regeneration using hiPSCs.

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