Reprogramming of Human Fibroblasts to Induced Pluripotent Stem Cells with Sleeping Beauty Transposon-Based Stable Gene Delivery

2016 ◽  
pp. 419-427 ◽  
Author(s):  
Attila Sebe ◽  
Zoltán Ivics
Keyword(s):  
Stem Cells ◽  
Gene Delivery ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Fibroblasts ◽  
Sleeping Beauty ◽  
Stable Gene ◽  
Sleeping Beauty Transposon ◽  
Induced Pluripotent

Generation of induced pluripotent stem cells from human foetal fibroblasts using the Sleeping Beauty transposon gene delivery system

2013 ◽  
Vol 86 (1-2) ◽  
pp. 30-37 ◽  
Author(s):  
Richard P. Davis ◽  
Csilla Nemes ◽  
Eszter Varga ◽  
Christian Freund ◽  
Georgios Kosmidis ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Delivery ◽  
Induced Pluripotent Stem Cells ◽  
Delivery System ◽  
Pluripotent Stem Cells ◽  
Sleeping Beauty ◽  
Gene Delivery System ◽  
Sleeping Beauty Transposon ◽  
Induced Pluripotent

scholarly journals Generation of Neuronal Progenitor Cells and Neurons from Mouse Sleeping Beauty Transposon–Generated Induced Pluripotent Stem Cells

2012 ◽  
Vol 14 (5) ◽  
pp. 390-397 ◽  
Author(s):  
Nuttha Klincumhom ◽  
Melinda K. Pirity ◽  
Sara Berzsenyi ◽  
Olga Ujhelly ◽  
Suchitra Muenthaisong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Progenitor Cells ◽  
Pluripotent Stem Cells ◽  
Sleeping Beauty ◽  
Sleeping Beauty Transposon ◽  
Neuronal Progenitor Cells ◽  
Neuronal Progenitor ◽  
Induced Pluripotent

Emerging potential of transposons for gene therapy and generation of induced pluripotent stem cells

Blood ◽  
2009 ◽  
Vol 114 (8) ◽  
pp. 1461-1468 ◽  
Author(s):  
Thierry VandenDriessche ◽  
Zoltán Ivics ◽  
Zsuzsanna Izsvák ◽  
Marinee K. L. Chuah
Keyword(s):  
Stem Cells ◽  
Gene Therapy ◽  
Gene Transfer ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
B Cell Lymphoma ◽  
Sleeping Beauty ◽  
Target Cells ◽  
Stable Gene ◽  
Induced Pluripotent

AbstractEffective gene therapy requires robust delivery of the desired genes into the relevant target cells, long-term gene expression, and minimal risks of secondary effects. The development of efficient and safe nonviral vectors would greatly facilitate clinical gene therapy studies. However, nonviral gene transfer approaches typically result in only limited stable gene transfer efficiencies in most primary cells. The use of nonviral gene delivery approaches in conjunction with the latest generation transposon technology based on Sleeping Beauty (SB) or piggyBac transposons may potentially overcome some of these limitations. In particular, a large-scale genetic screen in mammalian cells yielded a novel hyperactive SB transposase, resulting in robust and stable gene marking in vivo after hematopoietic reconstitution with CD34+ hematopoietic stem/progenitor cells in mouse models. Moreover, the first-in-man clinical trial has recently been approved to use redirected T cells engineered with SB for gene therapy of B-cell lymphoma. Finally, induced pluripotent stem cells could be generated after genetic reprogramming with piggyBac transposons encoding reprogramming factors. These recent developments underscore the emerging potential of transposons in gene therapy applications and induced pluripotent stem generation for regenerative medicine.

scholarly journals Sleeping Beauty transposon-based system for cellular reprogramming and targeted gene insertion in induced pluripotent stem cells

2012 ◽  
Vol 41 (3) ◽  
pp. 1829-1847 ◽  
Author(s):  
Ivana Grabundzija ◽  
Jichang Wang ◽  
Attila Sebe ◽  
Zsuzsanna Erdei ◽  
Robert Kajdi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Sleeping Beauty ◽  
Cellular Reprogramming ◽  
Sleeping Beauty Transposon ◽  
Gene Insertion ◽  
Induced Pluripotent

Generation of mouse induced pluripotent stem cells from different genetic backgrounds using Sleeping beauty transposon mediated gene transfer

2012 ◽  
Vol 318 (19) ◽  
pp. 2482-2489 ◽  
Author(s):  
Suchitra Muenthaisong ◽  
Olga Ujhelly ◽  
Zsuzsanna Polgar ◽  
Eszter Varga ◽  
Zoltan Ivics ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Transfer ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Sleeping Beauty ◽  
Sleeping Beauty Transposon ◽  
Induced Pluripotent

An insight into non-integrative gene delivery approaches to generate transgene-free induced pluripotent stem cells

Gene ◽  
2019 ◽  
Vol 686 ◽  
pp. 146-159 ◽  
Author(s):  
Krishna Kumar Haridhasapavalan ◽  
Manash P. Borgohain ◽  
Chandrima Dey ◽  
Bitan Saha ◽  
Gloria Narayan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Delivery ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent ◽  
Insight Into

Non-viral reprogramming of fibroblasts into induced pluripotent stem cells by Sleeping Beauty and piggyBac transposons

2014 ◽  
Vol 450 (1) ◽  
pp. 581-587 ◽  
Author(s):  
Thirumala R. Talluri ◽  
Dharmendra Kumar ◽  
Silke Glage ◽  
Wiebke Garrels ◽  
Zoltan Ivics ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Sleeping Beauty ◽  
Induced Pluripotent
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