Three-dimensional growth matrix for human embryonic stem cell-derived neuronal cells

2012 ◽  
Vol 8 (3) ◽  
pp. 186-194 ◽  
Author(s):  
Laura Ylä-Outinen ◽  
Tiina Joki ◽  
Mari Varjola ◽  
Heli Skottman ◽  
Susanna Narkilahti
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Neuronal Cells ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Dimensional Growth

Human embryonic stem cell-derived neuronal cells form spontaneously active neuronal networks in vitro

2009 ◽  
Vol 218 (1) ◽  
pp. 109-116 ◽  
Author(s):  
Teemu J. Heikkilä ◽  
Laura Ylä-Outinen ◽  
Jarno M.A. Tanskanen ◽  
Riikka S. Lappalainen ◽  
Heli Skottman ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Neuronal Networks ◽  
Neuronal Cells ◽  
Embryonic Stem

scholarly journals Hydrogel platform for in vitro three-dimensional assembly of human stem cell-derived β cells and endothelial cells

2019 ◽  
Author(s):  
Punn Augsornworawat ◽  
Leonardo Velazco-Cruz ◽  
Jiwon Song ◽  
Jeffrey R. Millman
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Three Dimensional ◽  
Embryonic Stem ◽  
Cell Types ◽  
Diabetic Patients ◽  
Β Cells

AbstractDifferentiation of stem cells into functional replacement cells and tissues is a major goal of the regenerative medicine field. However, one limitation has been organization of differentiated cells into multi-cellular, three-dimensional assemblies. The islets of Langerhans contain many endocrine and non-endocrine cell types, such as insulin-producing β cells and endothelial cells. Transplantation of exogenous islets into diabetic patients can serve as a cell replacement therapy, replacing the need for patients to inject themselves with insulin, but the number of available islets from cadaveric donors is low. We have developed a strategy of assembling human embryonic stem cell-derived β cells with endothelial cells into three-dimensional aggregates on a hydrogel. The resulting islet organoids express β cell markers and are functional, capable of undergoing glucose-stimulated insulin secretion. These results provide a platform for evaluating the effects of the islet tissue microenvironment on human embryonic stem cell-derived β cells and other islet endocrine cells to develop tissue engineered islets.

Enhanced Functions of Human Embryonic Stem Cell-derived Hepatocyte-like Cells on Three-dimensional Nanofibrillar Surfaces

2010 ◽  
Vol 6 (4) ◽  
pp. 601-610 ◽  
Author(s):  
Zahra Farzaneh ◽  
Behshad Pournasr ◽  
Marzeih Ebrahimi ◽  
Nasser Aghdami ◽  
Hossein Baharvand
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Three Dimensional ◽  
Embryonic Stem

scholarly journals Transcriptome analysis reveals determinant stages controlling human embryonic stem cell commitment to neuronal cells

2017 ◽  
Vol 292 (48) ◽  
pp. 19590-19604 ◽  
Author(s):  
Yuanyuan Li ◽  
Ran Wang ◽  
Nan Qiao ◽  
Guangdun Peng ◽  
Ke Zhang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Transcriptome Analysis ◽  
Human Embryonic Stem Cell ◽  
Neuronal Cells ◽  
Embryonic Stem ◽  
Cell Commitment
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