Accelerating bone defects healing in calvarial defect model using 3D cultured bone marrow‐derived mesenchymal stem cells on demineralized bone particle scaffold

2020 ◽  
Vol 14 (4) ◽  
pp. 563-574
Author(s):  
Jin Woo Kim ◽  
Jong Ho Park ◽  
Thangavelu Muthukumar ◽  
Eun Yeong Shin ◽  
Myeong Eun Shin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Defects ◽  
Calvarial Defect ◽  
Defect Model ◽  
Bone Particle ◽  
Demineralized Bone

scholarly journals Senolytics improve bone forming potential of bone marrow mesenchymal stem cells from aged mice

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Yueying Zhou ◽  
Xiaonan Xin ◽  
Lichao Wang ◽  
Binsheng Wang ◽  
Li Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Potential ◽  
Calvarial Defect ◽  
Defect Model ◽  
Aged Mice ◽  
Marrow Mesenchymal Stem Cells

AbstractThe osteogenic potential of bone marrow mesenchymal stem cells (BMSCs) declines dramatically with aging. By using a calvarial defect model, we showed that a senolytic cocktail (dasatinib+quercetin; D + Q) improved osteogenic capacity of aged BMSC both in vitro and in vivo. The study presented a model to assess strategies to improve bone-forming potential on aged BMSCs. D + Q might hold promise for improving BMSC function in aged populations.

scholarly journals Sinusoidal electromagnetic fields accelerate bone regeneration by boosting the multifunctionality of bone marrow mesenchymal stem cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Weigang Li ◽  
Wenbin Liu ◽  
Wei Wang ◽  
Jiachen Wang ◽  
Tian Ma ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Electromagnetic Fields ◽  
Bone Defects ◽  
Cell Scaffolds ◽  
Marrow Mesenchymal Stem Cells ◽  
Calvarial Defects

Abstract Background The repair of critical-sized bone defects is always a challenging problem. Electromagnetic fields (EMFs), used as a physiotherapy for bone defects, have been suspected to cause potential hazards to human health due to the long-term exposure. To optimize the application of EMF while avoiding its adverse effects, a combination of EMF and tissue engineering techniques is critical. Furthermore, a deeper understanding of the mechanism of action of EMF will lead to better applications in the future. Methods In this research, bone marrow mesenchymal stem cells (BMSCs) seeded on 3D-printed scaffolds were treated with sinusoidal EMFs in vitro. Then, 5.5 mm critical-sized calvarial defects were created in rats, and the cell scaffolds were implanted into the defects. In addition, the molecular and cellular mechanisms by which EMFs regulate BMSCs were explored with various approaches to gain deeper insight into the effects of EMFs. Results The cell scaffolds treated with EMF successfully accelerated the repair of critical-sized calvarial defects. Further studies revealed that EMF could not directly induce the differentiation of BMSCs but improved the sensitivity of BMSCs to BMP signals by upregulating the quantity of specific BMP (bone morphogenetic protein) receptors. Once these receptors receive BMP signals from the surrounding milieu, a cascade of reactions is initiated to promote osteogenic differentiation via the BMP/Smad signalling pathway. Moreover, the cytokines secreted by BMSCs treated with EMF can better facilitate angiogenesis and osteoimmunomodulation which play fundamental roles in bone regeneration. Conclusion In summary, EMF can promote the osteogenic potential of BMSCs and enhance the paracrine function of BMSCs to facilitate bone regeneration. These findings highlight the profound impact of EMF on tissue engineering and provide a new strategy for the clinical treatment of bone defects.

scholarly journals Mechanism of Action of Icariin in Bone Marrow Mesenchymal Stem Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Aofei Yang ◽  
Chaochao Yu ◽  
Qilin Lu ◽  
Hao Li ◽  
Zhanghua Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Mechanism Of Action ◽  
Underlying Mechanism ◽  
Bone Defects ◽  
Clinical Use ◽  
Regenerative Therapy ◽  
Herba Epimedii ◽  
Marrow Mesenchymal Stem Cells

Osteoporosis, femoral head necrosis, and congenital bone defects are orthopedic disorders characterized by reduced bone generation and insufficient bone mass. Bone regenerative therapy primarily relies on the bone marrow mesenchymal stem cells (BMSCs) and their ability to differentiate osteogenically. Icariin (ICA) is the active ingredient of Herba epimedii, a common herb used in traditional Chinese medicine (TCM) formulations, and can effectively enhance BMSC proliferation and osteogenesis. However, the underlying mechanism of ICA action in BMSCs is not completely clear. In this review, we provide an overview of the studies on the role and mechanism of action of ICA in BMSCs, to provide greater insights into its potential clinical use in bone regeneration.

Improved Osteogenesis by HVEM-Expressing Allogenic Bone Marrow-Derived Mesenchymal Stem Cells in an Immune Activation Condition and Mouse Femoral Defect Model

2018 ◽  
Vol 24 (15-16) ◽  
pp. 1167-1178 ◽  
Author(s):  
Zhigang Rong ◽  
Fei Zhang ◽  
Zhengdong Wang ◽  
Weifeng He ◽  
Shiwu Dong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Immune Activation ◽  
Defect Model ◽  
Activation Condition ◽  
Allogenic Bone ◽  
Allogenic Bone Marrow ◽  
Femoral Defect

scholarly journals Author Correction: Repair of bone defects with prefabricated vascularized bone grafts and double-labeled bone marrow-derived mesenchymal stem cells in a rat model

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Xiao-Rui Jiang ◽  
Hui-Ying Yang ◽  
Xin-Xin Zhang ◽  
Guo-Dong Lin ◽  
Yong-Chun Meng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Rat Model ◽  
Bone Grafts ◽  
Bone Defects ◽  
Vascularized Bone

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Enhancement of Bone Regeneration Using Osteogenic-Induced Adipose- Derived Stem Cells Combined with Demineralized Bone Matrix in a Rat Critically-Sized Calvarial Defect Model

2012 ◽  
Vol 7 (3) ◽  
pp. 165-172 ◽  
Author(s):  
Hyun Peel Kim ◽  
Yi-hwa Ji ◽  
Seung Chul Rhee ◽  
Eun Sang Dhong ◽  
Seung Ha Park ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Adipose Derived Stem Cells ◽  
Calvarial Defect ◽  
Defect Model ◽  
Demineralized Bone

Comparison of autogenic and allogenic bone marrow derived mesenchymal stem cells for repair of segmental bone defects in rabbits

2013 ◽  
Vol 94 (3) ◽  
pp. 743-752 ◽  
Author(s):  
Rahul Kumar Udehiya ◽  
Amarpal ◽  
H.P. Aithal ◽  
P. Kinjavdekar ◽  
A.M. Pawde ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Defects ◽  
Allogenic Bone ◽  
Allogenic Bone Marrow ◽  
Segmental Bone ◽  
Segmental Bone Defects

Repairing Effect of a Nano-Artificial Bone Combined with HIF-1α Transfected Bone Marrow Mesenchymal Stem Cells for Rabbit Radial Bone Defects

2016 ◽  
Vol 6 (10) ◽  
pp. 767-774 ◽  
Author(s):  
Lei Yang ◽  
Weimin Zhu ◽  
Jiaming Cui ◽  
Jianghong Huang ◽  
Fupeng Liao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Defects ◽  
Artificial Bone ◽  
Marrow Mesenchymal Stem Cells ◽  
Repairing Effect ◽  
Radial Bone

Bone tissue engineering using polyetherketoneketone scaffolds combined with autologous mesenchymal stem cells in a sheep calvarial defect model

2016 ◽  
Vol 44 (8) ◽  
pp. 985-994 ◽  
Author(s):  
Carina Adamzyk ◽  
Paul Kachel ◽  
Mareike Hoss ◽  
Felix Gremse ◽  
Ali Modabber ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Calvarial Defect ◽  
Defect Model ◽  
Autologous Mesenchymal Stem Cells
Sign in / Sign up

Export Citation Format

Share Document