P18.15: The usefulness of ovarian volume using 3D vocal transvaginal ultrasound, antral follicle count and age as predictors of menopause in pre- and postmenopausal women

Abstract Background: Intake of resveratrol has been associated with improved ovarian morphology under in vitro and in the animal models; however, this finding has not been confirmed in trials. The aim of our study was, therefore, to use a placebo-controlled approach with the detailed assessment of the ovarian morphology by applying transvaginal ultrasound to examine the effectiveness of this therapeutic approach in this group of women. Methods: Forty-one women with polycystic ovary syndrome (PCOS) were randomly assigned (1:1) to 3 months of daily 1000 mg resveratrol or placebo. Random assignment was done by blocked randomisation. Our primary endpoints were the change in the ovarian volume, stromal area and antral follicle count per ovary (FNPO) from the baseline to 3 months. Secondary endpoints were improvement in the distribution of follicles and ovarian echogenicity. Differences between the resveratrol and control groups were evaluated by Chi-square, fisher’s exact test and repeated-measures of ANOVA. Results: The mean age of all participants was 28.61 ± 4.99 years, with the mean BMI of 28.26 ± 5.62 kg/m2. Resveratrol therapy, as compared with placebo, was associated with a significantly higher rate of improvement in the ovarian morphology (p= 0.02). Women who received resveratrol had a more dominant follicle than those getting placebo, with a significant reduction in the ovarian volume (p<0.05). However, the number of FNPO, stromal area, ovarian echogenicity and distribution of follicles were not significantly altered (P>0.05). Conclusions: Treatment with resveratrol significantly reduced the ovarian volume and PCOM, thus suggesting a disease-modifying effect in PCOS. Trial registration: IRCT, IRCT2017061917139N2. Registered 7 July 2017, http://irct.ir/trial/15836.

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Granulocyte colony stimulating factor in COS-IUI cycles

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20191200 ◽

2019 ◽

Vol 8 (4) ◽

pp. 1463

Author(s):

Pradnya Devdas Shetty ◽

Nikita Lad ◽

Pallavi Vishwekar ◽

Mamta Vijay Shivtare

Keyword(s):

Pregnancy Rate ◽

Transvaginal Ultrasound ◽

Clomiphene Citrate ◽

Antral Follicle ◽

Antral Follicle Count ◽

Intrauterine Pregnancy ◽

Minimum Thickness ◽

Assisted Reproductive Technique ◽

Thin Endometrium ◽

Β Hcg

Background: An unresolved assisted reproductive technique problem is the unresponsive, thin endometrium. Approximately 0.6%-0.8% of patients do not reach the minimum thickness. Using endometrial co culture, G-CSF>130pg/mL was associated with significantly improved pregnancy rate in ART cycles. This is a retrospective study that included all unexplained infertility cycles with controlled ovulation stimulation –IUI protocols. Aim was to note the effects of G-CSF on thin endometrium and pregnancy rate in G-CSF administered COS-IUI cycles.Methods: This study was done in the IVF department of Dr D Y Patil University, Navi Mumbai, India. Thin endometrium was defined as ET<7mm on transvaginal ultrasound. Clomiphene citrate was used for ovulation induction in strengths of 100mg or 50mg on day 2 of their cycle based on the antral follicle count. Trigger used was injection 10,000µg urinary hCG. On the same day when the trigger injection was given, 300 units G-CSF was instilled into the uterus. Post 36 hours IUI was done under aseptic precautions .After 16 days β-hCG levels were done to determine whether there is a pregnancy.Results: In present study,200 COS-IUI cycles were analysed.50 cycles showed a thin endometrium and in them G-CSF was used. The chemical pregnancy rates was 32%, the intrauterine pregnancy rate was 28%, ectopic pregnancy rate was 4%.Conclusions: Present study concluded that G-CSF increases ET significantly in COS-IUI cycles in the event of thin endometrium. In view of small cohort size further larger randomized controlled trials may be required to substantiate the above conclusions.

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Reproductive capacity in iron overloaded women with thalassemia major

Blood ◽

10.1182/blood-2011-06-360271 ◽

2011 ◽

Vol 118 (10) ◽

pp. 2878-2881 ◽

Cited By ~ 35

Author(s):

Sylvia T. Singer ◽

Elliott P. Vichinsky ◽

Ginny Gildengorin ◽

Jereon van Disseldorp ◽

Mitchell Rosen ◽

...

Keyword(s):

Antral Follicle ◽

Thalassemia Major ◽

Antral Follicle Count ◽

Reproductive Capacity ◽

Gonadotropin Secretion ◽

Ovarian Volume ◽

Cardiac Iron ◽

Labile Iron ◽

Sensitive Marker

Abstract The pathophysiology of iron-induced compromised fertility in women with thalassemia major (TM) was evaluated in 26 adult TM females. Low gonadotropin secretion resulted in reduced ovarian antral follicle count and ovarian volume, but levels of anti-müllerian hormone (AMH), a sensitive marker for ovarian reserve independent of gonadotropin effect, were mostly normal. AMH correlated with non–transferrin-bound iron (NTBI), suggesting a role of labile iron in the pathogenesis of decreased reproductive capacity, possibly occurring in parallel to cardiac iron toxicity, as cardiac iron was associated with the presence of amenorrhea and with NTBI levels. AMH emerges as an important biomarker for assessment of reproductive capacity in TM, demonstrating that fertility is preserved in the majority of those younger than 30 to 35 years. AMH can be useful in future studies aiming at improved chelation for fertility preservation, whereas NTBI and labile plasma iron may be valuable for monitoring iron effect on the reproductive system.

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138 Relationships Between Antral Follicle Count, Ovarian Volume, Pre-Antral Follicle Number, and Oocyte Quality

Reproduction Fertility and Development ◽

10.1071/rdv30n1ab138 ◽

2018 ◽

Vol 30 (1) ◽

pp. 209

Author(s):

G. L. Vasconcelos ◽

R. Maculan ◽

N. Alves ◽

A. L. A. P. L. Ribeiro ◽

A. W. B. Silva ◽

...

Keyword(s):

Ovarian Follicle ◽

Primordial Follicle ◽

Antral Follicle ◽

Oocyte Quality ◽

Antral Follicle Count ◽

Primary Follicle ◽

Ovarian Volume ◽

Primordial Follicles ◽

Antral Follicles ◽

Grade 3

The objective was to evaluate the possible relationships between AFC, ovarian volume, ovarian follicle reserve and oocyte quality in abattoir-derived ovaries (experiment 1) and in cows (experiment 2) submitted to OPU. Antral follicle counts of ≥25, 16 to 24, and ≤ 16 were used to define AFC classes as high (HAFC), intermediate (IAFC), and low (LAFC) in both experiments. In experiment 1, after antral follicles were aspirated, abattoir ovaries (n = 10 per AFC class) were processed by conventional histology and pre-antral follicles were counted within primordial, primary, secondary, and tertiary classes and classified as either healthy or degenerate under regular microscopy (Cushman et al. 1999). In experiment 2, HAFC (n = 42), IAFC (n = 34), and LAFC (n = 29) cows were submitted to OPU and oocytes classified as grades 1, 2, and 3 or degenerate (IETS, 2010). Antral follicles (≥3 mm in diameter) were counted by ultrasonography. Data were analysed by GENMOD and GLM procedures of SAS (SAS Institute Inc., Cary, NC, USA) after transformations, when required. In experiment 1, mean normal primordial follicle number was higher (P < 0.001) in HAFC (137.0 ± 1.6)a compared with IAFC (52.6 ± 1.9)b and LAFC (20.2 ± 5.3)c ovaries. However, the mean number of degenerate primordial follicles was lower (P < 0.001) in low count ovaries (2.4 ± 0.6) compared with HAFC (19.0 ± 4.7) and IAFC (16.4 ± 1.5, P < 0.001). Normal primary follicle number was higher in the HAFC compared with IAFC and LAFC ovarian classes (86.2 ± 7.0a v. 34.6 ± 5.1b and 14.4 ± 3.3c, respectively; P < 0.01). Degenerate primary follicles were higher in the HAFC compared with LAFC ovarian class (16.8 ± 6.5 v. 5.2 ± 2.64; P < 0.05). Normal secondary follicle number was also higher in the HAFC compared to LAFC ovarian classes (25.2 ± 7.67 v. 2.4 ± 0.8; P < 0.05). The number of degenerate secondary follicles differed (P < 0.01) only between the IAFC and the LAFC ovarian classes (0.6 ± 0.4 and 7.2 ± 2.4, respectively), which were similar (P > 0.5) to the HAFC class (3.8 ± 1.0). In experiment 2, grade 1, 2, and 3 oocytes, viable oocytes, and ovarian volume (mm3) were higher (P < 0.001) in HAFC compared with IAFC and LAFC cows (grade 1: 7.9 ± 0.6a, 4.9 ± 0.7b and 3.3 ± 0.7c; grade 2: 4.0 ± 0.4a, 2.8 ± 0.4b and 1.2c; grade 3: 2.1 ± 0.4a, 2.5 ± 0.4a and 1.3 ± 0.5b, respectively; viable oocytes: 16.3 ± 1.1a, 13.1 ± 1.2b, and 8.1 ± 1.3c, respectively; (volumes: 12.6 ± 0.7a, 10.1 ± 0.8b, and 8.1 ± 0.9c, respectively). In conclusion, high AFC is linked to a higher follicular reserve, oocyte quality, and ovarian volume. It is safe to apply AFC in the selection of bovine females without compromising oocyte or pre-antral follicular population qualities.

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Ovarian volume and antral follicle count for the prediction of low and hyper responders with in vitro fertilization

Reproductive Biology and Endocrinology ◽

10.1186/1477-7827-5-9 ◽

2007 ◽

Vol 5 (1) ◽

Cited By ~ 71

Author(s):

Janet Kwee ◽

Mariet E Elting ◽

Roel Schats ◽

Joseph McDonnell ◽

Cornelis B Lambalk

Keyword(s):

In Vitro Fertilization ◽

Antral Follicle ◽

Antral Follicle Count ◽

Ovarian Volume ◽

Vitro Fertilization

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Ovarian volume and antral follicle count assessed by MRI and transvaginal ultrasonography: a methodological study

Acta Radiologica ◽

10.1177/0284185113495835 ◽

2014 ◽

Vol 55 (2) ◽

pp. 248-256 ◽

Cited By ~ 15

Author(s):

Henrik Leonhardt ◽

Berit Gull ◽

Elisabet Stener-Victorin ◽

Mikael Hellström

Keyword(s):

Antral Follicle ◽

Antral Follicle Count ◽

Transvaginal Ultrasonography ◽

Methodological Study ◽

Ovarian Volume

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Intraobserver and Interobserver Reproducibility of Ovarian Volume, Antral Follicle Count, and Vascularity Indices Obtained With Transvaginal 3-Dimensional Ultrasonography, Power Doppler Angiography, and the Virtual Organ Computer-Aided Analysis Imaging Pr

Journal of Ultrasound in Medicine ◽

10.7863/jum.2005.24.9.1279 ◽

2005 ◽

Vol 24 (9) ◽

pp. 1279-1287 ◽

Cited By ~ 57

Author(s):

Luis T. Mercé ◽

Belén Gómez ◽

Virginia Engels ◽

Santiago Bau ◽

José M. Bajo

Keyword(s):

Power Doppler ◽

Antral Follicle ◽

Antral Follicle Count ◽

Interobserver Reproducibility ◽

Ovarian Volume ◽

3 Dimensional ◽

Computer Aided Analysis ◽

Computer Aided

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Left–right differences in ovarian volume and antral follicle count in 1423 women of reproductive age

Gynecological Endocrinology ◽

10.1080/09513590.2016.1259406 ◽

2016 ◽

Vol 33 (4) ◽

pp. 320-323 ◽

Cited By ~ 6

Author(s):

Anne-Sofie Korsholm ◽

Helene Westring Hvidman ◽

Janne Gasseholm Bentzen ◽

Anders Nyboe Andersen ◽

Kathrine Birch Petersen

Keyword(s):

Antral Follicle ◽

Antral Follicle Count ◽

Reproductive Age ◽

Women Of Reproductive Age ◽

Ovarian Volume

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Relationship between anti-Müllerian hormone and antral follicle count across the menstrual cycle using the Beckman Coulter Access assay in comparison with Gen II manual assay

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2016-0609 ◽

2017 ◽

Vol 55 (7) ◽

Cited By ~ 7

Author(s):

Julia Schiffner ◽

Judith Roos ◽

David Broomhead ◽

Joseph van Helden ◽

Erhard Godehardt ◽

...

Keyword(s):

Menstrual Cycle ◽

Transvaginal Ultrasound ◽

Ovarian Follicle ◽

Antral Follicle ◽

Antral Follicle Count ◽

Cycle Number ◽

Antral Follicles ◽

Menstruating Women ◽

Conversion Equation ◽

Antral Follicle Counts

AbstractBackground:The study aim was to validate Beckman Coulter’s fully automated Access Immunoassay System (BC Access assay) for anti-Müllerian hormone (AMH) and compare it with Beckman Coulter’s Modified Manual Generation II assay (BC Mod Gen II), with regard to cycle AMH fluctuations and antral follicle counts.Methods:During one complete menstrual cycle, transvaginal ultrasound was performed on regularly menstruating women (n=39; 18–40years) every 2 days until the dominant ovarian follicle reached 16mm, then daily until observed ovulation; blood samples were collected throughout the cycle. Number and size of antral follicles was determined and AMH levels measured using both assays.Results:AMH levels measured by the BC Access assay vary over ovulatory menstrual cycles, with a statistically significant pre-ovulatory decrease from –5 to +2 days around objective ovulation. Mean luteal AMH levels were significantly lower (–7.99%) than mean follicular levels but increased again towards the end of the luteal phase. Antral follicle count can be estimated from AMH (ng/mL, BC Access assay) concentrations on any follicular phase day. BC Access assay-obtained AMH values are considerably lower compared with the BC Mod Gen II assay (–19% on average); conversion equation: AMH BC Access (ng/mL)=0.85 [AMH BC Mod Gen II (ng/mL)]Conclusions:AMH levels vary throughout the cycle, independently of assay utilised. A formula can be used to convert BC Access assay-obtained AMH levels to BC Mod Gen II values. The number of antral follicles can be consistently estimated from pre-ovulatory AMH levels using either assay.

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