A Simple Method for Determining Kinetic Constants of Slow, Tight-Binding Inhibition

1993 ◽  
Vol 213 (2) ◽  
pp. 370-377 ◽  
Author(s):  
Z.X. Wang
Keyword(s):  
Tight Binding ◽  
Kinetic Constants ◽  
Simple Method ◽  
Binding Inhibition

scholarly journals Accurate quantitative determination of affinity and binding kinetics for tight binding inhibition of xanthine oxidase

2021 ◽  
Vol 139 ◽  
pp. 111664
Author(s):  
Haiyang Yang ◽  
Xueyan Li ◽  
Gang Li ◽  
Huating Huang ◽  
Wenning Yang ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Xanthine Oxidase ◽  
Tight Binding ◽  
Binding Kinetics ◽  
Binding Inhibition

scholarly journals Theoretical treatment of tight-binding inhibition of an enzyme. Ribonuclease inhibitor as special case

1988 ◽  
Vol 253 (2) ◽  
pp. 517-522 ◽  
Author(s):  
J M Fominaya ◽  
J M García-Segura ◽  
M Ferreras ◽  
J G Gavilanes
Keyword(s):  
Tight Binding ◽  
Theoretical Treatment ◽  
Inhibitor Protein ◽  
Ribonuclease Inhibitor ◽  
Rat Testis ◽  
Binding Inhibition ◽  
Different Types ◽  
Functional Consequences ◽  
Inhibitor System ◽  
Special Case

A general treatment of very tight-binding inhibition is described. It was applied to purified endogenous RNAase inhibitor from rat testis. This treatment discriminates among the different types of inhibition and allows for calculation of the inhibition parameters. When very tight-binding inhibitions are studied at similar molar concentrations of both enzyme and inhibitor, a further approach is required. This is also described and applied to the RNAase inhibitor. A Ki value of 3.2 x 10(-12) M was found for this inhibitor protein. On the basis of this result, it was considered inappropriate to classify this type of inhibitor in terms of competitive or non-competitive, as has been done for such inhibitors so far. Functional consequences of this analysis are discussed for the RNAase-RNAase inhibitor system.

Tight-binding inhibition of jack bean α-mannosidase by glycoimidazole clusters

2019 ◽  
Vol 17 (23) ◽  
pp. 5801-5817 ◽  
Author(s):  
Maëva M. Pichon ◽  
Fabien Stauffert ◽  
Anne Bodlenner ◽  
Philippe Compain
Keyword(s):  
Tight Binding ◽  
Jack Bean ◽  
Processing Enzyme ◽  
Binding Inhibition

Examples of multimeric inhibitors displaying tight binding inhibition of a carbohydrate-processing enzyme are presented.

scholarly journals A re-evaluation of the kinetic equations for hyperbolic tight-binding inhibition

1988 ◽  
Vol 254 (1) ◽  
pp. 311-312 ◽  
Author(s):  
S E Szedlacsek ◽  
V Ostafe ◽  
M Serban ◽  
M O Vlad
Keyword(s):  
Tight Binding ◽  
Kinetic Equations ◽  
Binding Inhibition
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