The determination of kinetic constants governing the slow,tight-binding inhibition of enzyme-catalysed reactions

1986 ◽  
Vol 874 (1) ◽  
pp. 44-53 ◽  
Author(s):  
Michael J. Sculley ◽  
John F. Morrison
Keyword(s):  
Tight Binding ◽  
Kinetic Constants ◽  
Binding Inhibition

scholarly journals Accurate quantitative determination of affinity and binding kinetics for tight binding inhibition of xanthine oxidase

2021 ◽  
Vol 139 ◽  
pp. 111664
Author(s):  
Haiyang Yang ◽  
Xueyan Li ◽  
Gang Li ◽  
Huating Huang ◽  
Wenning Yang ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Xanthine Oxidase ◽  
Tight Binding ◽  
Binding Kinetics ◽  
Binding Inhibition

High-Throughput Screening of Enzyme Inhibitors: Automatic Determination of Tight-Binding Inhibition Constants

2000 ◽  
Vol 281 (1) ◽  
pp. 62-67 ◽  
Author(s):  
Petr Kuzmič ◽  
Steve Sideris ◽  
Lynne M. Cregar ◽  
Kyle C. Elrod ◽  
Kenneth D. Rice ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Enzyme Inhibitors ◽  
Tight Binding ◽  
Automatic Determination ◽  
Binding Inhibition ◽  
Inhibition Constants

KINETIC CONSTANTS DETERMINATION OF PETROLEUM REFINERY EFFLUENT TREATMENT IN A UASB REACTOR USING RSM

2017 ◽  
Vol 16 (1) ◽  
pp. 121-130 ◽  
Author(s):  
Seyyed Mohammad Mousavi ◽  
Seyed Omid Rastegar ◽  
Seyed Abbas Shojaosadati ◽  
Soheila Sheibani
Keyword(s):  
Petroleum Refinery ◽  
Kinetic Constants ◽  
Effluent Treatment ◽  
Uasb Reactor ◽  
Refinery Effluent ◽  
Petroleum Refinery Effluent

scholarly journals Simulation of extracellular nucleotide hydrolysis and determination of kinetic constants for the ectonucleotidases.

1986 ◽  
Vol 261 (33) ◽  
pp. 15505-15507
Author(s):  
L L Slakey ◽  
K Cosimini ◽  
J P Earls ◽  
C Thomas ◽  
E L Gordon
Keyword(s):  
Kinetic Constants ◽  
Extracellular Nucleotide ◽  
Nucleotide Hydrolysis

scholarly journals Theoretical treatment of tight-binding inhibition of an enzyme. Ribonuclease inhibitor as special case

1988 ◽  
Vol 253 (2) ◽  
pp. 517-522 ◽  
Author(s):  
J M Fominaya ◽  
J M García-Segura ◽  
M Ferreras ◽  
J G Gavilanes
Keyword(s):  
Tight Binding ◽  
Theoretical Treatment ◽  
Inhibitor Protein ◽  
Ribonuclease Inhibitor ◽  
Rat Testis ◽  
Binding Inhibition ◽  
Different Types ◽  
Functional Consequences ◽  
Inhibitor System ◽  
Special Case

A general treatment of very tight-binding inhibition is described. It was applied to purified endogenous RNAase inhibitor from rat testis. This treatment discriminates among the different types of inhibition and allows for calculation of the inhibition parameters. When very tight-binding inhibitions are studied at similar molar concentrations of both enzyme and inhibitor, a further approach is required. This is also described and applied to the RNAase inhibitor. A Ki value of 3.2 x 10(-12) M was found for this inhibitor protein. On the basis of this result, it was considered inappropriate to classify this type of inhibitor in terms of competitive or non-competitive, as has been done for such inhibitors so far. Functional consequences of this analysis are discussed for the RNAase-RNAase inhibitor system.

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