Theoretical Analysis of Protein Concentration Determination Using Biosensor Technology under Conditions of Partial Mass Transport Limitation

1997 ◽  
Vol 249 (2) ◽  
pp. 153-164 ◽  
Author(s):  
Lars L.H. Christensen
2004 ◽  
Vol 457-460 ◽  
pp. 67-70 ◽  
Author(s):  
A.V. Kulik ◽  
M.V. Bogdanov ◽  
S.Yu. Karpov ◽  
M.S. Ramm ◽  
Yuri N. Makarov

Author(s):  
Eszter Csibra ◽  
Guy-Bart Stan

FPCount is a complete protocol for fluorescent protein calibration, consisting of: 1. FP expression/purification using Thermo's HisPur Cobalt Resin. 2. FP concentration determination in a microplate reader. 3. FP fluorescence quantification in a microplate reader. Results can be analysed with the corresponding R package, FPCountR. --- Summary 1. Expression 2. Harvesting/Washing 3. Lysis 4. Fractionation 5. Gel1: Verification of Expression/Fractions 6. Purification 7. Gel2: Verification of Purification 8. Protein concentration and buffer exchange 9. Quantification of FP concentration (part1) 10. Quantification of FP fluorescence 11. Quantification of FP concentration (part2) 12. Protein Storage 13. Calibration of Plate Reader


1992 ◽  
Vol 20 (3) ◽  
pp. 195-199 ◽  
Author(s):  
G.D. Svoboda ◽  
J. Zhou ◽  
P.S. Cheng ◽  
M. Asif ◽  
D.L. Distelrath ◽  
...  

Lab on a Chip ◽  
2015 ◽  
Vol 15 (17) ◽  
pp. 3424-3427 ◽  
Author(s):  
Shiv Acharya ◽  
Shayson Edwards ◽  
Jacob Schmidt

Nanopore detection of specific protein species using carrier DNA – Quantitative protein concentration determination using aptamers – Protein folding studied with unfoldase-coupled nanopores.


1978 ◽  
Vol 33 (5) ◽  
pp. 455-458 ◽  
Author(s):  
J. C. Wang ◽  
R. F. Wood ◽  
P. P. Pronko

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