Enhancement by Baclofen of the Gs-Coupled Receptor-Mediated cAMP Production inXenopusOocytes Expressing Rat Brain Cortex Poly (A)+RNA: A Role of G-Protein βγ Subunits

1997 ◽  
Vol 241 (2) ◽  
pp. 476-480 ◽  
Author(s):  
Yasuhito Uezono ◽  
Yoko Ueda ◽  
Susumu Ueno ◽  
Izumi Shibuya ◽  
Nobuyuki Yanagihara ◽  
...  
Keyword(s):  
Rat Brain ◽  
G Protein ◽  
Brain Cortex ◽  
Camp Production ◽  
Rat Brain Cortex

Mechanisms and role of the rhythmogenesis in the sensorimotor processes of the rat brain cortex

2010 ◽  
Vol 77 (3) ◽  
pp. 214-214
Author(s):  
Maria V. Skornyakova ◽  
Alexander G. Sukhov ◽  
Lilia A. Belichenko ◽  
Alexander K. Logvinov ◽  
Larisa V. Lysenko ◽  
...  
Keyword(s):  
Rat Brain ◽  
Brain Cortex ◽  
Rat Brain Cortex ◽  
Sensorimotor Processes

Increased baclofen-stimulated G protein coupling and deactivation in rat brain cortex during development

2004 ◽  
Vol 151 (1-2) ◽  
pp. 67-73 ◽  
Author(s):  
Jiri Stöhr ◽  
Lenka Bourova ◽  
Lucie Hejnova ◽  
Ivanna Ihnatovych ◽  
Jiri Novotny ◽  
...  
Keyword(s):  
Rat Brain ◽  
G Protein ◽  
Brain Cortex ◽  
G Protein Coupling ◽  
Protein Coupling ◽  
Rat Brain Cortex

Kainic acid-induced seizure activity alters the mRNA expression and G-protein activation of the opioid/nociceptin receptors in the rat brain cortex

2013 ◽  
Vol 105 (1-2) ◽  
pp. 13-19 ◽  
Author(s):  
Ezgi Turunc Bayrakdar ◽  
Engin Bojnik ◽  
Guliz Armagan ◽  
Lutfiye Kanit ◽  
Sandor Benyhe ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Rat Brain ◽  
G Protein ◽  
Kainic Acid ◽  
Brain Cortex ◽  
Seizure Activity ◽  
Protein Activation ◽  
Rat Brain Cortex ◽  
G Protein Activation

scholarly journals Ontogenetic Development of GABAB-Receptor Signaling Cascade in Plasma Membranes Isolated From Rat Brain Cortex; the Number of GABAB-Receptors Is High Already Shortly After the Birth

2012 ◽  
pp. 629-635 ◽  
Author(s):  
D. KAGAN ◽  
K. DLOUHÁ ◽  
L. ROUBALOVÁ ◽  
P. SVOBODA
Keyword(s):  
Rat Brain ◽  
G Protein ◽  
Plasma Membranes ◽  
Brain Cortex ◽  
Binding Assay ◽  
Gabab Receptor ◽  
Gabab Receptors ◽  
Protein Activity ◽  
Rat Brain Cortex ◽  
Gtpγs Binding

Our data indicate the significant intrinsic efficacy of GABAB-receptors in rat brain cortex already at birth (PD1, PD2). Subsequently, baclofen- and SKF97541-stimulated G-protein activity, measured by agonist-stimulated, high-affinity [35S]GTPγS binding assay, was increased; the highest level of both baclofen and SKF97541-stimulated [35S]GTPγS binding was detected between PD10 and PD15. In older rats, baclofen- and SKF97541-stimulated [35S]GTPγS binding was continuously decreased so, that the level in adult, 90-days old animals, was not different from that in newborn animals. The potency of G-protein response to baclofen (characterized by EC50 values) was also high at birth but unchanged by further postnatal development. An individual variance among different agonists was observed in this respect as the potency of SKF97541 response was decreased between the birth and adulthood. Accordingly, the highest plasma membrane density of GABAB-R, determined by saturation binding assay with antagonist [3H]CGP54626, was measured in 1-day old animals (2.27±0.08 pmol · mg-1). The further development was reflected in a decrease of [3H]CGP54626 binding as the Bmax values of 1.38±0.05 and 0.93±0.04 pmol · mg-1 were determined in PM isolated from 13- and 90-days old rats, respectively.

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