scholarly journals The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence

2009 ◽  
Vol 1788 (2) ◽  
pp. 324-332 ◽  
Author(s):  
J. Sýkora ◽  
L. Bouřová ◽  
M. Hof ◽  
P. Svoboda
Keyword(s):  
Rat Brain ◽  
G Protein ◽  
Plasma Membranes ◽  
Brain Cortex ◽  
Protein Activity ◽  
Rat Brain Cortex ◽  
Laurdan Fluorescence

scholarly journals Ontogenetic Development of GABAB-Receptor Signaling Cascade in Plasma Membranes Isolated From Rat Brain Cortex; the Number of GABAB-Receptors Is High Already Shortly After the Birth

2012 ◽  
pp. 629-635 ◽  
Author(s):  
D. KAGAN ◽  
K. DLOUHÁ ◽  
L. ROUBALOVÁ ◽  
P. SVOBODA
Keyword(s):  
Rat Brain ◽  
G Protein ◽  
Plasma Membranes ◽  
Brain Cortex ◽  
Binding Assay ◽  
Gabab Receptor ◽  
Gabab Receptors ◽  
Protein Activity ◽  
Rat Brain Cortex ◽  
Gtpγs Binding

Our data indicate the significant intrinsic efficacy of GABAB-receptors in rat brain cortex already at birth (PD1, PD2). Subsequently, baclofen- and SKF97541-stimulated G-protein activity, measured by agonist-stimulated, high-affinity [35S]GTPγS binding assay, was increased; the highest level of both baclofen and SKF97541-stimulated [35S]GTPγS binding was detected between PD10 and PD15. In older rats, baclofen- and SKF97541-stimulated [35S]GTPγS binding was continuously decreased so, that the level in adult, 90-days old animals, was not different from that in newborn animals. The potency of G-protein response to baclofen (characterized by EC50 values) was also high at birth but unchanged by further postnatal development. An individual variance among different agonists was observed in this respect as the potency of SKF97541 response was decreased between the birth and adulthood. Accordingly, the highest plasma membrane density of GABAB-R, determined by saturation binding assay with antagonist [3H]CGP54626, was measured in 1-day old animals (2.27±0.08 pmol · mg-1). The further development was reflected in a decrease of [3H]CGP54626 binding as the Bmax values of 1.38±0.05 and 0.93±0.04 pmol · mg-1 were determined in PM isolated from 13- and 90-days old rats, respectively.

Increased baclofen-stimulated G protein coupling and deactivation in rat brain cortex during development

2004 ◽  
Vol 151 (1-2) ◽  
pp. 67-73 ◽  
Author(s):  
Jiri Stöhr ◽  
Lenka Bourova ◽  
Lucie Hejnova ◽  
Ivanna Ihnatovych ◽  
Jiri Novotny ◽  
...  
Keyword(s):  
Rat Brain ◽  
G Protein ◽  
Brain Cortex ◽  
G Protein Coupling ◽  
Protein Coupling ◽  
Rat Brain Cortex

Kainic acid-induced seizure activity alters the mRNA expression and G-protein activation of the opioid/nociceptin receptors in the rat brain cortex

2013 ◽  
Vol 105 (1-2) ◽  
pp. 13-19 ◽  
Author(s):  
Ezgi Turunc Bayrakdar ◽  
Engin Bojnik ◽  
Guliz Armagan ◽  
Lutfiye Kanit ◽  
Sandor Benyhe ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Rat Brain ◽  
G Protein ◽  
Kainic Acid ◽  
Brain Cortex ◽  
Seizure Activity ◽  
Protein Activation ◽  
Rat Brain Cortex ◽  
G Protein Activation

Effects of anesthetics on sodium uptake into rat brain cortex in vitro

1972 ◽  
Vol 21 (12) ◽  
pp. 1763-1773 ◽  
Author(s):  
R. Shankaran ◽  
J.H. Quastel
Keyword(s):  
Rat Brain ◽  
Brain Cortex ◽  
Sodium Uptake ◽  
Rat Brain Cortex

The expression changes of Numblike in rat brain cortex after traumatic brain injury

2012 ◽  
Vol 43 (2) ◽  
pp. 195-201 ◽  
Author(s):  
Shengyang Jiang ◽  
Xiaohong Wu ◽  
Yaohua Yan ◽  
Jian Xu ◽  
Bai Shao ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Rat Brain ◽  
Brain Cortex ◽  
Rat Brain Cortex

Aluminum accumulation and membrane fluidity alteration in synaptosomes isolated from rat brain cortex following aluminum ingestion: effect of cholesterol

2002 ◽  
Vol 44 (2) ◽  
pp. 181-193 ◽  
Author(s):  
Virgı́lia S Silva ◽  
J Miguel Cordeiro ◽  
Manuel J Matos ◽  
Catarina R Oliveira ◽  
Paula P Gonçalves
Keyword(s):  
Rat Brain ◽  
Membrane Fluidity ◽  
Brain Cortex ◽  
Rat Brain Cortex ◽  
Aluminum Accumulation

Identity of inhibitory presynaptic 5-hydroxytryptamine (5-HT) autoreceptors in the rat brain cortex with 5-HT1B binding sites

1986 ◽  
Vol 332 (1) ◽  
pp. 1-7 ◽  
Author(s):  
G. Engel ◽  
M. G�thert ◽  
D. Hoyer ◽  
E. Schlicker ◽  
K. Hillenbrand
Keyword(s):  
Rat Brain ◽  
Binding Sites ◽  
Brain Cortex ◽  
Rat Brain Cortex
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