The Effects of Temperature and Oleic Acid on Murine Memory and Virgin T Cell Activation: Interleukin-2 Secretion and Interleukin-2 Receptor Expression

1993 ◽  
Vol 152 (1) ◽  
pp. 35-48 ◽  
Author(s):  
R.Mark Wooten ◽  
L.William Clem ◽  
Jan E. Bly
Keyword(s):  
Oleic Acid ◽  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Interleukin 2 ◽  
Receptor Expression ◽  
Interleukin 2 Receptor ◽  
Effects Of Temperature

scholarly journals Human T cell activation. II. A new activation pathway used by a major T cell population via a disulfide-bonded dimer of a 44 kilodalton polypeptide (9.3 antigen).

1985 ◽  
Vol 161 (6) ◽  
pp. 1513-1524 ◽  
Author(s):  
T Hara ◽  
S M Fu ◽  
J A Hansen
Keyword(s):  
Cell Proliferation ◽  
T Cells ◽  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Interleukin 2 ◽  
Cell Subset ◽  
Receptor Expression ◽  
T Cell Proliferation ◽  
Activation Pathway

In previous studies (17-21), monoclonal antibody (mAb) 9.3 has been shown to react with a major population of human T cells, which include T4+ helper/inducer T cells and T8+ cytotoxic T cells. In this investigation, mAb 9.3 was shown to precipitate a disulfide-bonded dimer of a 44 kD polypeptide. Comodulation experiments showed that this molecule is not linked to T3/Ti or T11 antigens. mAb 9.3 was capable of inducing T cell proliferation in the presence of 12-o-tetradecanoyl phorbol-13-acetate (TPA). This effect was monocyte-independent. T cell activation with mAb 9.3 and TPA was associated with increases in interleukin 2(IL-2) receptor expression and IL-2 secretion. mAb 9.3 did not activate T cells, even with the addition of IL-1 or IL-2. Modulation of the T3 complex did not abolish mAb 9.3-induced T cell proliferation in the presence of TPA. These results suggest that the 9.3 antigen may serve as a receptor for an activation pathway restricted to a T cell subset.

Deregulated T cell activation and autoimmunity in mice lacking interleukin-2 receptor beta

Science ◽  
1995 ◽  
Vol 268 (5216) ◽  
pp. 1472-1476 ◽  
Author(s):  
H Suzuki ◽  
T. Kundig ◽  
C Furlonger ◽  
A Wakeham ◽  
E Timms ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Interleukin 2 ◽  
Interleukin 2 Receptor ◽  
Receptor Beta

scholarly journals The Interleukin 2 Receptor α Chain/CD25 Promoter Is a Target for Nuclear Factor of Activated T Cells

1998 ◽  
Vol 188 (7) ◽  
pp. 1369-1373 ◽  
Author(s):  
Kai Schuh ◽  
Thomas Twardzik ◽  
Burkhard Kneitz ◽  
Jörg Heyer ◽  
Anneliese Schimpl ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Activation ◽  
Cell Activation ◽  
Interleukin 2 ◽  
Receptor Expression ◽  
Transcriptional Level ◽  
Nuclear Factor ◽  
Close Link ◽  
Α Chain

The expression of the murine interleukin (IL)-2 receptor α chain/CD25 is strongly induced at the transcriptional level after T cell activation. We show here that nuclear factor of activated T cell (NF-AT) factors are involved in the control of CD25 promoter induction in T cells. NF-ATp and NF-ATc bind to two sites around positions −585 and −650 located upstream of the proximal CD25 promoter. Immediately 3′ from these NF-AT motifs, nonconsensus sites are located for the binding of AP-1–like factors. Mutations of sites that suppress NF-AT binding impair the induction and strong NF-ATp–mediated transactivation of the CD25 promoter in T cells. In T lymphocytes from NF-ATp–deficient mice, the expression of CD25 is severely impaired, leading to a delayed IL-2 receptor expression after T cell receptor (TCR)/CD3 stimulation. Our data indicate an important role for NF-AT in the faithful expression of high affinity IL-2 receptors and a close link between the TCR-mediated induction of IL-2 and IL-2 receptor α chain promoters, both of which are regulated by NF-AT factors.

Sign in / Sign up

Export Citation Format

Share Document