p53 Gene Family p51(p63)-Encoded, Secondary Transactivator p51B(TAp63α) Occurs without Forming an Immunoprecipitable Complex with MDM2, but Responds to Genotoxic Stress by Accumulation

p53 gene family members in humans and other organisms encode a large number of protein isoforms whose functions are largely undefined. Using Drosophila as a model, we find that a p53B isoform is expressed predominantly in the germline where it colocalizes with p53A into subnuclear bodies. It is only p53A, however, that mediates the apoptotic response to ionizing radiation in the germline and soma. In contrast, p53A and p53B are both required for the normal repair of meiotic DNA breaks, an activity that is more crucial when meiotic recombination is defective. We find that in oocytes with persistent DNA breaks p53A is also required to activate a meiotic pachytene checkpoint. Our findings indicate that Drosophila p53 isoforms have DNA lesion and cell type-specific functions, with parallels to the functions of mammalian p53 family members in the genotoxic stress response and oocyte quality control.

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The Emerging p53 Gene Family

JNCI Journal of the National Cancer Institute ◽

10.1093/jnci/91.7.594 ◽

1999 ◽

Vol 91 (7) ◽

pp. 594-598 ◽

Cited By ~ 95

Author(s):

W. G. Kaelin

Keyword(s):

Gene Family ◽

P53 Gene

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p53 Gene Family

Encyclopedic Reference of Cancer ◽

10.1007/3-540-30683-8_1255 ◽

2006 ◽

pp. 668-669

Author(s):

William Kaelin

Keyword(s):

Gene Family ◽

P53 Gene

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The p53 Gene Family: Control of Cell Proliferation and Developmental Programs

Biochemistry (Moscow) ◽

10.1007/s10541-005-0210-4 ◽

2005 ◽

Vol 70 (9) ◽

pp. 955-971 ◽

Cited By ~ 4

Author(s):

A. A. Morgunkova

Keyword(s):

Cell Proliferation ◽

Gene Family ◽

P53 Gene ◽

Developmental Programs ◽

Family Control

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The Transcription Express Characteristics of Several Genes in the Process of Bombyx mori Ovarian Carcinoma

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.796.39 ◽

2013 ◽

Vol 796 ◽

pp. 39-42

Author(s):

Xie Yi ◽

Li Bing

Keyword(s):

Ovarian Carcinoma ◽

Gene Family ◽

Bombyx Mori ◽

Cell Lines ◽

P53 Gene ◽

Ovary Cell ◽

Ovary Cells ◽

Bmn Cell ◽

Cyclin Gene ◽

Materials Used

Bombyx mori cell line (BmN) comes fromBombyx moriovary cell subculture. In order to study the change of several genes transcription in the process ofBombyx moriovary cells primary culture and subculture, we usedBombyx moriovary organizations and BmN cell lines as research materials, used Real Time fluorescent quantitative RT-PCR to detect cyclin gene family (CyclinA, CyclinB, CyclinB3, CyclinE, CyclinL1), p53 and Telomerase genes transcription level in the ovary and BmN cell lines, and took Actin3 gene as reference to dispose the results. The results showed that in theBombyx moriBmN cell lines the expression of CyclinA, CyclinB, CyclinB3, CyclinE, CyclinL1 and Telomerase genes were higher than those in the ovary. The expression of CyclinB in the BmN was more then 3.8 which was 76 times higher than that in the ovary; The expression of p53 gene in the BmN cell was lower than that in the ovary; The expression of Telomerase gene in the BmN cell was higher than that in the ovary. The results accumulated a reliable data for further study on the the role of cyclin gene family, p53 gene, and Telomerase gene in the process ofBombyx moriovarian carcinoma.

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