Plasmodium yoelii: Efficient in Vitro Invasion and Complete Development of Sporozoites in Mouse Hepatic Cell Lines

2000 ◽  
Vol 96 (4) ◽  
pp. 257-259 ◽  
Author(s):  
Maria M Mota ◽  
Ana Rodriguez
Keyword(s):  
Cell Lines ◽  
Plasmodium Yoelii ◽  
Hepatic Cell ◽  
Complete Development ◽  
In Vitro Invasion ◽  
Hepatic Cell Lines

Hepcidin Inhibition by Modified Heparins without Anticoagulant Activity

Blood ◽  
2012 ◽  
Vol 120 (21) ◽  
pp. 483-483
Author(s):  
Maura Poli ◽  
Domenico Girelli ◽  
Annamaria Naggi ◽  
Natascia Campostrini ◽  
Dario Finazzi ◽  
...  
Keyword(s):  
Cell Lines ◽  
Conflicts Of Interest ◽  
Anticoagulant Activity ◽  
Hepatic Cell ◽  
Maximum Effect ◽  
Hepcidin Expression ◽  
Serum Hepcidin ◽  
Hepatic Cell Lines

Abstract Abstract 483 Background. There is an increasing interest in the development of pharmacological agents able to modulate hepcidin, the peptide hormone that critically regulates iron metabolism. In particular, hepcidin antagonist may have a therapeutic role in the anemia of chronic diseases, where hepcidin levels are often increased by pro-inflammatory cytokines. We previously demonstrated that heparin is a potent inhibitor of hepcidin expression in hepatic cell lines, probably by interfering with BMP/HJV/SMAD signalling, and that it was also effective in reducing hepcidin expression in mice (Poli M, Blood 2011; 117:997–1004). Since the therapeutic use of heparin for hepcidin modulation is hampered by its strong anticoagulant activity, we were interested in evaluating modified heparins without such activity. Methods. Heparins modified to inactivate the antithrombin binding site, with different molecular weight and degree of sulfation, were supplied to hepatic cell lines and mice to evaluate their potential modulation of hepcidin expression. We analysed their interference with the BMP/SMAD signalling, as well as serum hepcidin levels in mice by mass spectrometry. Results. Over 20 modified heparins were initially screened by evaluating their dose-dependent suppression of hepcidin expression before and after BMP induction. All of them showed a certain degree of anti-hepcidin activity, with two glycol-split molecules being as potent as classical unfractionated heparin. These two molecules suppressed BMP/SMAD signalling in HepG2 cells at pharmacological concentrations with maximum inhibition after 6 hours. In mice, treatments with 20 or 60 mg/Kg did not affect coagulation but strongly reduced liver pSMAD, hepcidin mRNA and serum hepcidin. Again, the maximum effect on liver hepcidin expression was observed 6 hours after the injection. This effect was observed also in conditions of high hepcidin caused by experimental inflammation or iron overload. Conclusions. Some non-anticoagulant heparins have strong anti-hepcidin activity both in vitro and in vivo, and may represent promising hepcidin antagonist with potential therapeutic applications. Disclosures: No relevant conflicts of interest to declare.

Impact of counterions on the toxicity of metallic nanoparticles on hepatic cell lines in vitro

2017 ◽  
Vol 280 ◽  
pp. S216
Author(s):  
Albert Braeuning ◽  
Holger Sieg ◽  
Linda Böhmert ◽  
Dajana Lichtenstein ◽  
Alfonso Lampen
Keyword(s):  
Cell Lines ◽  
Metallic Nanoparticles ◽  
Hepatic Cell ◽  
Hepatic Cell Lines

Evaluation of the Cytotoxic Effects of MEIC Chemicals 31–50 on Primary Culture of Rat Hepatocytes and Hepatic and Non-hepatic Cell Lines

1997 ◽  
Vol 25 (4) ◽  
pp. 423-436
Author(s):  
Xavier Ponsoda ◽  
Cristina Núñez ◽  
José Vicente Castell ◽  
Maria José Gómez-Lechón
Keyword(s):  
Primary Culture ◽  
Cell Lines ◽  
Rat Hepatocytes ◽  
In Vitro Cytotoxicity ◽  
Hepatic Cell ◽  
Cellular Systems ◽  
Human Toxicity ◽  
Response Curves ◽  
Hepatic Cell Lines

The cytotoxicities of 20 chemicals (numbers 31–50) from the Multicenter Evaluation of In Vitro Cytotoxicity (MEIC) programme were assessed with a primary culture of rat hepatocytes and with two hepatic cell lines (Hep G2 and FaO) and one non-hepatic cell line (3T3). The cytotoxicities of the chemicals were evaluated by using the MTT test after the cells had been exposed to the chemicals for 24 hours. For a better evaluation of results, dose–response curves were mathematically linearised and cytotoxicity was expressed as IC50 values and IC10 values (the concentration causing 50% and 10% loss of cell viability, respectively). We found that all the compounds showed similar acute basal cytotoxicity in all four cellular systems (regardless of whether the cells were, or were not, metabolically competent or were or were not of human origin). When these results were used to predicit human toxicity in terms of a mathematical parameter (prediction error [PE]), we found that all four systems gave similar predictions of human toxicity. The best cytotoxicity parameter included in the PE calculation was the IC50/10, because of an underestimation of human toxicity by in vitro systems. However, when PEs were calculated for rodent toxicity, better results were obtained. Data from the literature obtained by using other experimental models for predicting human toxicity were analysed according to the same criteria. We conclude that cellular systems are better predictive tools for human toxicity than are prokaryotic cells or whole-organism models.

Development of an in vitro screening method of acute cytotoxicity of the pyrrolizidine alkaloid lasiocarpine in human and rodent hepatic cell lines by increasing susceptibility

2018 ◽  
Vol 217 ◽  
pp. 134-139
Author(s):  
Kristina Forsch ◽  
Verena Schöning ◽  
Lucia Disch ◽  
Beate Siewert ◽  
Matthias Unger ◽  
...  
Keyword(s):  
Cell Lines ◽  
Pyrrolizidine Alkaloid ◽  
Screening Method ◽  
Hepatic Cell ◽  
In Vitro Screening ◽  
Acute Cytotoxicity ◽  
Hepatic Cell Lines

In vitro screening of acute hepatic cytotoxicity of pyrrolizidine alkaloids in human and rodent hepatic cell lines

2017 ◽  
Author(s):  
K Forsch ◽  
B Siewert ◽  
L Disch ◽  
M Unger ◽  
J Drewe
Keyword(s):  
Cell Lines ◽  
Pyrrolizidine Alkaloids ◽  
Hepatic Cell ◽  
In Vitro Screening ◽  
Hepatic Cell Lines

scholarly journals Potential Antifibrotic and Angiostatic Impact of Idebenone, Carnosine and Vitamin E in Nano-Sized Titanium Dioxide-Induced Liver Injury

2015 ◽  
Vol 35 (6) ◽  
pp. 2402-2411 ◽  
Author(s):  
Samy A. Abdelazim ◽  
Hebatallah A. Darwish ◽  
Sanaa A. Ali ◽  
Maha Z. Rizk ◽  
Mai O. Kadry
Keyword(s):  
Titanium Dioxide ◽  
Vitamin E ◽  
Growth Factor ◽  
Cell Lines ◽  
Transforming Growth Factor ◽  
Hepatic Cell ◽  
Hepatic Tissue ◽  
Combination Regimen ◽  
Hepatic Cell Lines

Background/Aim: The present study investigated the in vitro and in vivo effects of individual and combined doses of idebenone, carnosine and vitamin E on ameliorating some of the biochemical indices of nano-sized titanium dioxide (n-TiO2) in mice liver. Methods: The in vitro cytotoxic effect of nano-sized anatase TiO2 (21 nm) on hepatic cell lines (HepG 2) was investigated. Additionally, n-TiO2 was orally administered (150 mg/kg/day) for 2 weeks, followed by a daily intragastric gavage of the aforementioned antioxidants for 1 month. Results: n-TiO2 induced significant cytotoxicity in hepatic cell lines and elevated the levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatic total antioxidant capacity (TAC) and nitrite/nitrate (NOx) levels. Meanwhile, glutathione-S-transferase (GST) activity was significantly reduced. Moreover, RT-PCR and western blot analysis showed that n-TiO2 significantly altered the mRNA and protein expressions of transforming growth factor-beta (TGF-β1) and Smad-2, as well as vascular endothelium growth factor (VEGF). Histopathological examination of hepatic tissue reinforced these results.Conclusion: Idebenone, carnosine and vitamin E ameliorated the deviated parameters with the combination regimen demonstrating the most pronounced effect. Oxidative stress, liver fibrosis and angiogenesis may be implicated in n-TiO2-induced liver toxicity.

scholarly journals Immortalized Human Hepatic Cell Lines for In Vitro Testing and Research Purposes

2014 ◽  
pp. 53-76 ◽  
Author(s):  
Eva Ramboer ◽  
Tamara Vanhaecke ◽  
Vera Rogiers ◽  
Mathieu Vinken
Keyword(s):  
Cell Lines ◽  
Hepatic Cell ◽  
In Vitro Testing ◽  
Hepatic Cell Lines

In vitro screening of acute hepatic cytotoxicity of pyrrolizidine alkaloids in human and rodent hepatic cell lines

2017 ◽  
Vol 280 ◽  
pp. S197
Author(s):  
Kristina Forsch ◽  
Beate Siewert ◽  
Lucia Disch ◽  
Matthias Unger ◽  
Jürgen Drewe
Keyword(s):  
Cell Lines ◽  
Pyrrolizidine Alkaloids ◽  
Hepatic Cell ◽  
In Vitro Screening ◽  
Hepatic Cell Lines

Cytotoxicity of anisidine in hepatic cell lines

2015 ◽  
Vol 53 (01) ◽  
Author(s):  
S Caliskan ◽  
F Wewering ◽  
B Gerding ◽  
A Luch ◽  
S Zellmer
Keyword(s):  
Cell Lines ◽  
Hepatic Cell ◽  
Hepatic Cell Lines
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