Cloning and Mapping of the U2af1-rs2 Gene with a High Transmission Distortion in Interspecific Backcross Progeny

Abstract Transmission distortion is identified as a difference in transmission frequency of two alleles from the normal 1:1 Mendelian segregation in diploid organisms. Transmission distortion can extend over part or all of a chromosome. The recent development of interspecific mouse backcrosses has provided a powerful method for multilocus mapping of entire chromosomes in a single cross, and consequently for identifying distortions in allelic inheritance. We used an interspecific backcross of [(C57BL/6J x Mus spretus)F1 x C57BL/6J] mice to map molecular loci to mouse chromosome 2 and had previously found that the distal region of the chromosome showed distortions in allelic inheritance. We now report the mapping of five loci (Actc-1, D2Hgu1, His-1, Hox-4.1 and Neb) to chromosome 2, which, in addition to the Abl, Ada, B2m, Bmp-2a, Hc, Emv-15, Fshb, Hck-1, Pax-1, Pck-1, Spna-2 and Vim loci previously mapped in our interspecific backcross, serve as markers to measure allelic inheritance along approximately 75% of mouse chromosome 2. Statistical analyses are used to identify and delimit chromosomal regions showing transmission distortion and to determine whether there are sex-specific differences in allelic inheritance. These studies provide evidence for sex-specific differences in allelic inheritance for chromosome 2 and suggest biological explanations for this form of transmission distortion.

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Analysis of sex-chromosome aneuploidy in interspecific backcross progeny between the laboratory mouse strain C57BL/6 and Mus spretus

Cytogenetic and Genome Research ◽

10.1159/000133299 ◽

1992 ◽

Vol 60 (1) ◽

pp. 74-78 ◽

Cited By ~ 10

Author(s):

Y. Matsuda ◽

V.M. Chapman

Keyword(s):

Mouse Strain ◽

Sex Chromosome ◽

Laboratory Mouse ◽

Backcross Progeny ◽

Interspecific Backcross ◽

Sex Chromosome Aneuploidy ◽

Laboratory Mouse Strain ◽

Chromosome Aneuploidy

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High-transmission tunable metallic photonic crystal filters

10.1049/ic:20030166 ◽

2003 ◽

Author(s):

T.D. Drysdale

Keyword(s):

Photonic Crystal ◽

High Transmission ◽

Metallic Photonic Crystal

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PROTEIN POLYMORPHISMS, SEGREGATION IN GENETIC CROSSES AND GENETIC DISTANCES AMONG FISHES OF THE GENUS XIPHOPHORUS (POECILIIDAE)

Genetics ◽

10.1093/genetics/102.3.539 ◽

1982 ◽

Vol 102 (3) ◽

pp. 539-556

Author(s):

Don C Morizot ◽

Michael J Siciliano

Keyword(s):

Goodness Of Fit ◽

Inbred Strains ◽

Interspecific Backcross ◽

Genetic Distances ◽

Rapid Expansion ◽

Starch Gel Electrophoresis ◽

Lower Vertebrate ◽

Protein Coding ◽

Group I ◽

Starch Gel

ABSTRACT The products of 49 protein-coding loci were examined by starch gel electrophoresis for populational variation in six species of Xiphophorus fishes and/or segregation in intra- and interspecific backcross and intercross hybrids. Electrophoretic variation was observed for 29 of the 35 locus products in a survey of 42 population samples. The highest frequency of polymorphic loci observed in noninbred populations was 0.143. After ten or more generations of inbreeding, all loci studied were monomorphic. Inbred strains generally exhibited the commonest electrophoretic alleles of the population from which they were derived. An assessment of genetic distances among Xiphophorus populations reflected classical systematic relationships and suggested incipient subspeciation between X. maculatus from different drainages as well as several species groups. Thirty-three loci were analyzed with respect to segregation in hybrids. The goodness of fit of segregations to Mendelian expectations at all loci analyzed (except loci in linkage group I) is interpreted as evidence for high genetic compatibility of the genomes of Xiphophorus species. It is anticipated that these data will result in a rapid expansion of the assignment of protein-coding loci to linkage groups in these lower vertebrate species.

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Nonrandom Segregation of the Mouse Univalent X Chromosome: Evidence of Spindle-Mediated Meiotic Drive

Genetics ◽

10.1093/genetics/156.2.775 ◽

2000 ◽

Vol 156 (2) ◽

pp. 775-783 ◽

Cited By ~ 1

Author(s):

Renée LeMaire-Adkins ◽

Patricia A Hunt

Keyword(s):

X Chromosome ◽

Chromosome Abnormality ◽

Three Dimensional ◽

Fundamental Principle ◽

Mendelian Inheritance ◽

Segregation Behavior ◽

Chromosome Transmission ◽

Distortion Effect ◽

Random Segregation ◽

Transmission Distortion

Abstract A fundamental principle of Mendelian inheritance is random segregation of alleles to progeny; however, examples of distorted transmission either of specific alleles or of whole chromosomes have been described in a variety of species. In humans and mice, a distortion in chromosome transmission is often associated with a chromosome abnormality. One such example is the fertile XO female mouse. A transmission distortion effect that results in an excess of XX over XO daughters among the progeny of XO females has been recognized for nearly four decades. Utilizing contemporary methodology that combines immunofluorescence, FISH, and three-dimensional confocal microscopy, we have readdressed the meiotic segregation behavior of the single X chromosome in oocytes from XO females produced on two different inbred backgrounds. Our studies demonstrate that segregation of the univalent X chromosome at the first meiotic division is nonrandom, with preferential retention of the X chromosome in the oocyte in ∼60% of cells. We propose that this deviation from Mendelian expectations is facilitated by a spindle-mediated mechanism. This mechanism, which appears to be a general feature of the female meiotic process, has implications for the frequency of nondisjunction in our species.

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Recombinant inbred strain and interspecific backcross analysis of molecular markers flanking the murine agouti coat color locus.

Genetics ◽

10.1093/genetics/122.3.669 ◽

1989 ◽

Vol 122 (3) ◽

pp. 669-679

Author(s):

L D Siracusa ◽

A M Buchberg ◽

N G Copeland ◽

N A Jenkins

Keyword(s):

Molecular Markers ◽

Inbred Strain ◽

Mouse Chromosome ◽

Recombinant Inbred ◽

Recombinant Inbred Strain ◽

Interspecific Backcross ◽

Chromosome 2 ◽

Chromosome 4 ◽

Agouti Locus ◽

Mouse Chromosome 2

Abstract Recombinant inbred strain and interspecific backcross mice were used to create a molecular genetic linkage map of the distal portion of mouse chromosome 2. The orientation and distance of the Ada, Emv-13, Emv-15, Hck-1, Il-1a, Pck-1, Psp, Src-1 and Svp-1 loci from the beta 2-microglobulin locus and the agouti locus were established. Our mapping results have provided the identification of molecular markers both proximal and distal to the agouti locus. The recombinants obtained provide valuable resources for determining the direction of chromosome walking experiments designed to clone sequences at the agouti locus. Comparisons between the mouse and human genome maps suggest that the human homolog of the agouti locus resides on human chromosome 20q. Three loci not present on mouse chromosome 2 were also identified and were provisionally named Psp-2, Hck-2 and Hck-3. The Psp-2 locus maps to mouse chromosome 14. The Hck-2 locus maps near the centromere of mouse chromosome 4 and may identify the Lyn locus. The Hck-3 locus maps near the distal end of mouse chromosome 4 and may identify the Lck locus.

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Anchoring a genetic map of an interspecific backcross two family to the genome builds of Elaeis

Journal of Genetics ◽

10.1007/s12041-020-01240-8 ◽

2021 ◽

Vol 100 (1) ◽

Author(s):

KATIALISA KAMARUDDIN ◽

MAIZURA ITHNIN ◽

NGOOT-CHIN TING ◽

ZULKIFLI YAAKUB ◽

NIK SHAZANA NIK MOHD SANUSI ◽

...

Keyword(s):

Genetic Map ◽

Interspecific Backcross

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Genotyping cognate Plasmodium falciparum in humans and mosquitoes to estimate onward transmission of asymptomatic infections

Nature Communications ◽

10.1038/s41467-021-21269-2 ◽

2021 ◽

Vol 12 (1) ◽

Cited By ~ 2

Author(s):

Kelsey M. Sumner ◽

Elizabeth Freedman ◽

Lucy Abel ◽

Andrew Obala ◽

Brian W. Pence ◽

...

Keyword(s):

Plasmodium Falciparum ◽

Odds Ratio ◽

Asymptomatic Malaria ◽

Major Contributor ◽

Western Kenya ◽

High Transmission ◽

Transmission Reduction ◽

Asymptomatic Infections ◽

Infection Types ◽

Natural Settings

AbstractMalaria control may be enhanced by targeting reservoirs of Plasmodium falciparum transmission. One putative reservoir is asymptomatic malaria infections and the scale of their contribution to transmission in natural settings is not known. We assess the contribution of asymptomatic malaria to onward transmission using a 14-month longitudinal cohort of 239 participants in a high transmission site in Western Kenya. We identify P. falciparum in asymptomatically- and symptomatically-infected participants and naturally-fed mosquitoes from their households, genotype all parasites using deep sequencing of the parasite genes pfama1 and pfcsp, and use haplotypes to infer participant-to-mosquito transmission through a probabilistic model. In 1,242 infections (1,039 in people and 203 in mosquitoes), we observe 229 (pfcsp) and 348 (pfama1) unique parasite haplotypes. Using these to link human and mosquito infections, compared with symptomatic infections, asymptomatic infections more than double the odds of transmission to a mosquito among people with both infection types (Odds Ratio: 2.56; 95% Confidence Interval (CI): 1.36–4.81) and among all participants (OR 2.66; 95% CI: 2.05–3.47). Overall, 94.6% (95% CI: 93.1–95.8%) of mosquito infections likely resulted from asymptomatic infections. In high transmission areas, asymptomatic infections are the major contributor to mosquito infections and may be targeted as a component of transmission reduction.

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