Distribution and Ultrastructure of Plectin Arrays in Subclones of Rat Glioma C6 Cells Differing in Intermediate Filament Protein (Vimentin) Expression

1995 ◽  
Vol 115 (3) ◽  
pp. 304-317 ◽  
Author(s):  
Roland Foisner ◽  
Wolfgang Bohn ◽  
Klaus Mannweiler ◽  
Gerhard Wiche
Keyword(s):  
Intermediate Filament ◽  
Intermediate Filament Protein ◽  
C6 Cells ◽  
Vimentin Expression ◽  
Rat Glioma ◽  
Glioma C6 ◽  
Filament Protein

The presence or absence of a vimentin-type intermediate filament network affects the shape of the nucleus in human SW-13 cells

1994 ◽  
Vol 107 (6) ◽  
pp. 1593-1607 ◽  
Author(s):  
A.J. Sarria ◽  
J.G. Lieber ◽  
S.K. Nordeen ◽  
R.M. Evans
Keyword(s):  
Electron Microscopy ◽  
Intermediate Filament ◽  
Nuclear Morphology ◽  
Intermediate Filament Protein ◽  
Mosaic Pattern ◽  
Expression Plasmid ◽  
Vimentin Expression ◽  
Filament System ◽  
Filament Protein ◽  
Filament Network

Human SW-13 cells express the intermediate filament protein vimentin in a mosaic pattern (Hedberg, K. K. and Chen, L. B. (1986). Exp. Cell Res. 163, 509–517). We have isolated SW-13 clones that do (vim+) or do not (vim-) synthesize vimentin as analyzed using anti-intermediate filament immunofluorescence, electron microscopy and two-dimensional gel analysis of detergent-extracted preparations. Vimentin is the only cytoplasmic intermediate filament protein present in the vim+ cells, and the vim- cells do not contain any detectable cytoplasmic intermediate filament system. The presence or absence of intermediate filaments did not observably affect the distribution of mitochondria, endoplasmic reticulum, microtubules or actin stress fibers when these structures were visualized by fluorescence microscopy. However, electron microscopy and anti-lamin A/C immunofluorescence studies showed that nuclear morphology in vim- cells was frequently characterized by large folds or invaginations, while vim+ cells had a more regular or smooth nuclear shape. When vim- cells were transfected with a mouse vimentin expression plasmid, the synthesis of a mouse vimentin filament network restored the smooth nuclear morphology characteristic of vim+ cells. Conversely, when vim+ cells were transfected with a carboxy-terminally truncated mutant vimentin, expression of the mutant protein disrupted the organization of the endogenous vimentin filaments and resulted in nuclei with a prominently invaginated morphology. These results indicated that in SW-13 cells the vimentin filament system affects the shape of the nucleus.

Subclones of C6 rat glioma cells differing in intermediate filament protein expression

1991 ◽  
Vol 197 (2) ◽  
pp. 200-206 ◽  
Author(s):  
Kerstin Röser ◽  
Wolfgang Bohn ◽  
Günter Giese ◽  
Klaus Mannweiler
Keyword(s):  
Protein Expression ◽  
Intermediate Filament ◽  
Glioma Cells ◽  
Intermediate Filament Protein ◽  
Rat Glioma ◽  
Filament Protein

Cytoskeleton Architecture of C6 Rat Glioma Cell Subclones Differing in Intermediate Filament Protein Expression

1993 ◽  
Vol 111 (1) ◽  
pp. 48-58 ◽  
Author(s):  
Wolfgang Bohn ◽  
Kerstin Röser ◽  
Heinz Hohenberg ◽  
Klaus Mannweiler ◽  
Peter Traub
Keyword(s):  
Protein Expression ◽  
Intermediate Filament ◽  
Glioma Cell ◽  
Intermediate Filament Protein ◽  
Rat Glioma ◽  
Filament Protein ◽  
Cell Subclones

scholarly journals The Intermediate Filament Protein Vimentin Is a New Target for Epigallocatechin Gallate

2005 ◽  
Vol 280 (17) ◽  
pp. 16882-16890 ◽  
Author(s):  
Svetlana Ermakova ◽  
Bu Young Choi ◽  
Hong Seok Choi ◽  
Bong Seok Kang ◽  
Ann M. Bode ◽  
...  
Keyword(s):  
Intermediate Filament ◽  
Epigallocatechin Gallate ◽  
Intermediate Filament Protein ◽  
Filament Protein
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