The Tuberous Sclerosis 2 Gene Product Can Localize to Nuclei in a Phosphorylation-Dependent Manner

2001 ◽  
Vol 4 (6) ◽  
pp. 374-380 ◽  
Author(s):  
Dingyuan Lou ◽  
Nicole Griffith ◽  
Daniel J. Noonan
Keyword(s):  
Tuberous Sclerosis ◽  
Gene Product ◽  
Dependent Manner

scholarly journals Hamartin, the tuberous sclerosis complex 1 gene product, interacts with polo-like kinase 1 in a phosphorylation-dependent manner

2005 ◽  
Vol 15 (2) ◽  
pp. 287-297 ◽  
Author(s):  
Aristotelis Astrinidis ◽  
William Senapedis ◽  
Elizabeth P. Henske
Keyword(s):  
Tuberous Sclerosis ◽  
Gene Product ◽  
Tuberous Sclerosis Complex ◽  
Dependent Manner

Loss of tuberin, the tuberous-sclerosis-complex-2 gene product is associated with angiogenesis

2001 ◽  
Vol 28 (9) ◽  
pp. 470-475 ◽  
Author(s):  
Phuong-Anh Nguyen-Vu ◽  
Ingrid Fackler ◽  
Adelheid Rust ◽  
Jeffrey E. DeClue ◽  
Christian A. Sander ◽  
...  
Keyword(s):  
Tuberous Sclerosis ◽  
Gene Product ◽  
Tuberous Sclerosis Complex

scholarly journals The retinoblastoma gene product RB stimulates Sp1-mediated transcription by liberating Sp1 from a negative regulator.

1994 ◽  
Vol 14 (7) ◽  
pp. 4380-4389 ◽  
Author(s):  
L I Chen ◽  
T Nishinaka ◽  
K Kwan ◽  
I Kitabayashi ◽  
K Yokoyama ◽  
...  
Keyword(s):  
Dna Binding ◽  
Gene Product ◽  
Binding Activity ◽  
Negative Regulator ◽  
Control Element ◽  
Dependent Manner ◽  
Mobility Shift ◽  
Dna Binding Activity ◽  
Cell Extracts ◽  
Mobility Shift Assay

Studies have demonstrated that the retinoblastoma susceptibility gene product, RB, can either positively or negatively regulate expression of several genes through cis-acting elements in a cell-type-dependent manner. The nucleotide sequence of the retinoblastoma control element (RCE) motif, GCCACC or CCACCC, and the Sp1 consensus binding sequence, CCGCCC, can confer equal responsiveness to RB. Here, we report that RB activates transcription of the c-jun gene through the Sp1-binding site within the c-jun promoter. Preincubation of crude nuclear extracts with monoclonal antibodies to RB results in reduction of Sp1 complexes in a mobility shift assay, while addition of recombinant RB in mobility shift assay mixtures with CCL64 cell extracts leads to an enhancement of DNA-binding activity of SP1. These results suggest that RB is directly or indirectly involved in Sp1-DNA binding activity. A mechanism by which RB regulates transactivation is indicated by our detection of a heat-labile and protease-sensitive Sp1 negative regulator(s) (Sp1-I) that specifically inhibits Sp1 binding to a c-jun Sp1 site. This inhibition is reversed by addition of recombinant RB proteins, suggesting that RB stimulates Sp1-mediated transactivation by liberating Sp1 from Sp1-I. Additional evidence for Sp1-I involvement in Sp1-mediated transactivation was demonstrated by cotransfection of RB, GAL4-Sp1, and a GAL4-responsive template into CV-1 cells. Finally, we have identified Sp1-I, a approximately 20-kDa protein(s) that inhibits the Sp1 complexes from binding to DNA and that is also an RB-associated protein. These findings provide evidence for a functional link between two distinct classes of oncoproteins, RB and c-Jun, that are involved in the control of cell growth, and also define a novel mechanism for the regulation of c-jun expression.

scholarly journals The tuberous sclerosis-1 (TSC1) gene product hamartin suppresses cell growth and augments the expression of the TSC2 product tuberin by inhibiting its ubiquitination

Oncogene ◽  
2000 ◽  
Vol 19 (54) ◽  
pp. 6306-6316 ◽  
Author(s):  
Giovanna Benvenuto ◽  
Shaowei Li ◽  
Samantha J Brown ◽  
Richard Braverman ◽  
William C Vass ◽  
...  
Keyword(s):  
Cell Growth ◽  
Tuberous Sclerosis ◽  
Gene Product ◽  
Tsc1 Gene

scholarly journals Tuberous Sclerosis Complex 2 Gene Product Interacts with Human SMAD Proteins

2004 ◽  
Vol 279 (24) ◽  
pp. 25605-25613 ◽  
Author(s):  
Maria C. Birchenall-Roberts ◽  
Tao Fu ◽  
Ok-sun Bang ◽  
Michael Dambach ◽  
James H. Resau ◽  
...  
Keyword(s):  
Tuberous Sclerosis ◽  
Gene Product ◽  
Tuberous Sclerosis Complex ◽  
Smad Proteins

scholarly journals Loss of the B irt– H ogg– D ubé gene product folliculin induces longevity in a hypoxia‐inducible factor–dependent manner

Aging Cell ◽  
2013 ◽  
Vol 12 (4) ◽  
pp. 593-603 ◽  
Author(s):  
Hakam Gharbi ◽  
Francesca Fabretti ◽  
Puneet Bharill ◽  
Markus M. Rinschen ◽  
Sibylle Brinkkötter ◽  
...  
Keyword(s):  
Gene Product ◽  
Hypoxia Inducible Factor ◽  
Dependent Manner

scholarly journals Tuberous sclerosis causing mutants of the TSC2 gene product affect proliferation and p27 expression

Oncogene ◽  
2001 ◽  
Vol 20 (35) ◽  
pp. 4904-4909 ◽  
Author(s):  
Thomas Soucek ◽  
Margit Rosner ◽  
Angelina Miloloza ◽  
Marion Kubista ◽  
Jeremy P Cheadle ◽  
...  
Keyword(s):  
Tuberous Sclerosis ◽  
Gene Product ◽  
Tsc2 Gene

scholarly journals Modulation of Androgen Receptor Transactivation by the SWI3-Related Gene Product (SRG3) in Multiple Ways

2005 ◽  
Vol 25 (12) ◽  
pp. 4841-4852 ◽  
Author(s):  
Cheol Yi Hong ◽  
Ji Ho Suh ◽  
Kabsun Kim ◽  
Eun-Yeung Gong ◽  
Sung Ho Jeon ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Gene Product ◽  
Dependent Manner ◽  
Related Gene ◽  
Developmentally Regulated ◽  
Prostate Development ◽  
Mouse Prostate ◽  
Alternative Function ◽  
Regulatory Loop

ABSTRACT The SWI3-related gene product (SRG3), a component of the mouse SWI/SNF complex, has been suggested to have an alternative function. Here, we demonstrate that in the prostate transactivation of the androgen receptor (AR) is modulated by SRG3 in multiple ways. The expression of SRG3, which is developmentally regulated in the prostate, is induced by androgen through AR. SRG3 in turn enhances the transactivation of AR, providing a positive feedback regulatory loop. The SRG3 coactivation of AR transactivation is achieved through the recruitment of coactivator SRC-1, the protein level of which is upregulated by SRG3, providing another pathway of positive regulation. Interestingly, SRG3 coactivation of AR transactivation is fully functional in BRG1/BRM-deficient C33A cells and the AR/SRG3/SRC-1 complex formed in vivo contains neither BRG1 nor BRM protein, suggesting the possibility of an SRG3 function independent of the SWI/SNF complex. Importantly, the AR/SRG3/SRC-1 complex occupies androgen response elements on the endogenous SRG3 and PSA promoter in an androgen-dependent manner in mouse prostate and LNCaP cells, respectively, inducing gene expression. These results suggest that the multiple positive regulatory mechanisms of AR transactivation by SRG3 may be important for the rapid proliferation of prostate cells during prostate development and regeneration.

Identification and characterization of the Egr-1 gene product, a DNA-binding zinc finger protein induced by differentiation and growth signals

1990 ◽  
Vol 10 (5) ◽  
pp. 1931-1939
Author(s):  
X M Cao ◽  
R A Koski ◽  
A Gashler ◽  
M McKiernan ◽  
C F Morris ◽  
...  
Keyword(s):  
Gene Product ◽  
Zinc Finger Protein ◽  
Zinc Fingers ◽  
Early Response ◽  
Cell Fractionation ◽  
Dependent Manner ◽  
Finger Protein ◽  
Identification And Characterization

Egr-1 is an immediate-early response gene induced by diverse signals that initiate growth and differentiation. Its cDNA sequence predicts a protein with zinc fingers. We have generated an antiserum to the Egr-1 gene product and identified it as an 80-kilodalton short-lived protein in serum-stimulated mouse fibroblasts. The rat Egr-1 product has also been identified in nerve growth factor-induced PC12 cells. In addition, we show by cell fractionation and immunocytochemistry that the Egr-1 protein is located in the nucleus. We also demonstrate that it is phosphorylated. In vitro-generated Egr-1 protein binds with high affinity to the sequence CGCCCCCGC in a zinc-dependent manner.

scholarly journals Identification and characterization of the Egr-1 gene product, a DNA-binding zinc finger protein induced by differentiation and growth signals.

1990 ◽  
Vol 10 (5) ◽  
pp. 1931-1939 ◽  
Author(s):  
X M Cao ◽  
R A Koski ◽  
A Gashler ◽  
M McKiernan ◽  
C F Morris ◽  
...  
Keyword(s):  
Gene Product ◽  
Zinc Finger Protein ◽  
Zinc Fingers ◽  
Early Response ◽  
Cell Fractionation ◽  
Dependent Manner ◽  
Finger Protein ◽  
Identification And Characterization

Egr-1 is an immediate-early response gene induced by diverse signals that initiate growth and differentiation. Its cDNA sequence predicts a protein with zinc fingers. We have generated an antiserum to the Egr-1 gene product and identified it as an 80-kilodalton short-lived protein in serum-stimulated mouse fibroblasts. The rat Egr-1 product has also been identified in nerve growth factor-induced PC12 cells. In addition, we show by cell fractionation and immunocytochemistry that the Egr-1 protein is located in the nucleus. We also demonstrate that it is phosphorylated. In vitro-generated Egr-1 protein binds with high affinity to the sequence CGCCCCCGC in a zinc-dependent manner.

Sign in / Sign up

Export Citation Format

Share Document